ID   CP2K1_ONCMY             Reviewed;         504 AA.
AC   Q92090; O93304;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Cytochrome P450 2K1;
DE            EC=1.14.14.1;
DE   AltName: Full=CYPIIK1;
DE   AltName: Full=Cytochrome P450 LMC2;
GN   Name=cyp2k1;
OS   Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC   Salmonidae; Salmoninae; Oncorhynchus.
OX   NCBI_TaxID=8022;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   PubMed=8031145; DOI=10.1006/abbi.1994.1278;
RA   Buhler D.R., Yang Y.-H., Dreher T.W., Miranda C.L., Wang J.-L.;
RT   "Cloning and sequencing of the major rainbow trout constitutive cytochrome
RT   P450 (CYP2K1): identification of a new cytochrome P450 gene subfamily and
RT   its expression in mature rainbow trout liver and trunk kidney.";
RL   Arch. Biochem. Biophys. 312:45-51(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RA   Yang Y.-H., Buhler D.R.;
RT   "Cloning and sequencing of new cytochromes P450 from liver of rainbow
RT   trout.";
RL   Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an organic molecule + O2 + reduced [NADPH--hemoprotein
CC         reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein
CC         reductase]; Xref=Rhea:RHEA:17149, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC         COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:30879, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:142491; EC=1.14.14.1;
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}. Microsome membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- INDUCTION: Constitutively expressed.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; L11528; AAA52078.1; -; mRNA.
DR   EMBL; AF045052; AAC28309.1; -; mRNA.
DR   PIR; S45644; S45644.
DR   AlphaFoldDB; Q92090; -.
DR   SMR; Q92090; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070330; F:aromatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0046222; P:aflatoxin metabolic process; IMP:AgBase.
DR   GO; GO:0048252; P:lauric acid metabolic process; IDA:AgBase.
DR   GO; GO:0002933; P:lipid hydroxylation; IDA:AgBase.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW   Monooxygenase; Oxidoreductase.
FT   CHAIN           1..504
FT                   /note="Cytochrome P450 2K1"
FT                   /id="PRO_0000051773"
FT   BINDING         447
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
FT   VARIANT         6..7
FT                   /note="DI -> GL (in allele CYP2K1v2)"
FT   VARIANT         37
FT                   /note="E -> G (in allele CYP2K1v2)"
FT   VARIANT         79
FT                   /note="V -> F (in allele CYP2K1v2)"
FT   VARIANT         256
FT                   /note="I -> T (in allele CYP2K1v2)"
FT   VARIANT         261
FT                   /note="G -> S (in allele CYP2K1v2)"
FT   VARIANT         372
FT                   /note="N -> D (in allele CYP2K1v2)"
SQ   SEQUENCE   504 AA;  56792 MW;  3EFAE8D78D5EB21F CRC64;
     MSLIEDILQT SSTVTLLGTV LFLLVLYLRS SGSSSEEQGK EPPGPRPLPL LGNMLQLDLK
     KPYCTLCELS KKYGSIFTVH FGPKKVVVLA GYKTVKQALV NQAEDFGDRD ITPVFYDFNQ
     GHGILFANGD SWKEMRRFAL TNLRDFGMGK KGSEEKILEE IPYLIEVFEK HEGKAFDTTQ
     SVLYAVSNII SAIVYGSRFE YTDPLFTGMA DRAKESIHLT GSASIQMYNM FPWLGPWINN
     LTRLKKNIAD MKMEVIELVR GLKETLNPHM CRGFVDSFLV RKQTLEESGH MDSFYHDDNL
     VFSVGNLFSA GTDTTGTTLR WGLLLMTKYP HIQDQVQEEI SRVIGSRQTL VEDRKNLPYT
     DAVIHETQRL ANIVPMSVPH TTSRDVTFQG YFIKKGTSVI PLLTSVLQDD SEWESPNTFN
     PSHFLDEQGG FVKRDAFMAF SAGRRVCLGE GLARMELFLF FTSLLQRFRF SPPPGVTEDD
     LDLTPLLGFT LNPSPHQLCA VSRV