ID   CP2K4_ONCMY             Reviewed;         504 AA.
AC   O93297;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Cytochrome P450 2K4;
DE            EC=1.14.14.1;
DE   AltName: Full=CYPIIK4;
GN   Name=cyp2k4;
OS   Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC   Salmonidae; Salmoninae; Oncorhynchus.
OX   NCBI_TaxID=8022;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Kidney;
RA   Yang Y.-H., Andersson T.B., Ryu B.-W., Wang J.-L., Buhler D.R.;
RT   "CYP2K4: a new cytochrome P450 isoform from male trunk kidney of post-
RT   spawning rainbow trout.";
RL   Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an organic molecule + O2 + reduced [NADPH--hemoprotein
CC         reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein
CC         reductase]; Xref=Rhea:RHEA:17149, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC         COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:30879, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:142491; EC=1.14.14.1;
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}. Microsome membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF043296; AAC26492.1; -; mRNA.
DR   RefSeq; NP_001118227.1; NM_001124755.1.
DR   AlphaFoldDB; O93297; -.
DR   SMR; O93297; -.
DR   GeneID; 100142633; -.
DR   KEGG; omy:100142633; -.
DR   CTD; 100142633; -.
DR   OrthoDB; 702827at2759; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070330; F:aromatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW   Monooxygenase; Oxidoreductase.
FT   CHAIN           1..504
FT                   /note="Cytochrome P450 2K4"
FT                   /id="PRO_0000051775"
FT   BINDING         447
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   504 AA;  56734 MW;  AC4292C18617C4B1 CRC64;
     MSLIEGLLQT SSTVTLLGTV LFLLVPYLRS SGSSSEEQGK EPPGPRPLPL LGNMLQLDLK
     KPYCTLCELS KKYGSIFTVH FGSKKVVVLA GYKTVKQALV NQAEDFGERD ITPAFYDFNQ
     GHGILFTNGD SWKEMRRFAL TNLRDFGMGK KGSEEKILEE IPYLIEVLEK HEGKAFDTTQ
     SVHHAVSNII SAIVYGSRFE YTDPLFTGMV DRVNENVHLI GSASIQMYNM FPWLGPWINN
     LTRLKKNVAD LKMEVIELVR GLKETLNPHM CRGFVDSFLV RKQTLEESGK MDSFYHDDNL
     LFSIGNLFGA GTDTTGTTLR WGLLLMAKYP HIQDQVQEEI SRVIGSRQTL VEDRKNLPYT
     DAVIHETQRL ANISPMAVPH TTSRDVTFQG YFIKKGTSVI PLLMSVLQDD NEWESPNTFN
     PSHFLDEQGG FVKRDAFMAF SAGRRVCLGE GLARMELFLF FTSLLQRFRF SPPPGVTEDD
     LDLTPLLGFT LHPSPHQLCA VSRV