CP307_DROME
ID CP307_DROME Reviewed; 543 AA.
AC Q9VRM7; Q8SZ84;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 15-AUG-2003, sequence version 3.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Cytochrome P450 307a1;
DE EC=1.14.-.-;
DE AltName: Full=CYPCCCVIIA1;
DE AltName: Full=Protein spook;
GN Name=spo; Synonyms=Cyp307a1; ORFNames=CG10594;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Petryk A., Jarcho M., O'Connor M.B.;
RT "Cloning and characterization of Halloween group mutants.";
RL Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION.
RX PubMed=10328928; DOI=10.1006/dbio.1999.9235;
RA Hummel T., Schimmelpfeng K., Klambt C.;
RT "Commissure formation in the embryonic CNS of Drosophila.";
RL Dev. Biol. 209:381-398(1999).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-219, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Required for correct development of the embryonic midline
CC glial cells which are necessary for the formation of distinct segmental
CC commissures. {ECO:0000269|PubMed:10328928}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Member of the Halloween gene group.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL48675.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF484415; AAQ05973.1; -; mRNA.
DR EMBL; AE014296; AAF50766.3; -; Genomic_DNA.
DR EMBL; AY071053; AAL48675.1; ALT_FRAME; mRNA.
DR RefSeq; NP_001286943.1; NM_001300014.1.
DR RefSeq; NP_647975.2; NM_139718.3.
DR AlphaFoldDB; Q9VRM7; -.
DR SMR; Q9VRM7; -.
DR BioGRID; 64094; 5.
DR DIP; DIP-17643N; -.
DR IntAct; Q9VRM7; 4.
DR STRING; 7227.FBpp0076836; -.
DR iPTMnet; Q9VRM7; -.
DR PaxDb; Q9VRM7; -.
DR PRIDE; Q9VRM7; -.
DR EnsemblMetazoa; FBtr0077130; FBpp0076836; FBgn0003486.
DR EnsemblMetazoa; FBtr0346640; FBpp0312220; FBgn0003486.
DR GeneID; 38631; -.
DR KEGG; dme:Dmel_CG10594; -.
DR UCSC; CG10594-RA; d. melanogaster.
DR CTD; 38631; -.
DR FlyBase; FBgn0003486; spo.
DR VEuPathDB; VectorBase:FBgn0003486; -.
DR eggNOG; KOG0156; Eukaryota.
DR GeneTree; ENSGT00940000167305; -.
DR HOGENOM; CLU_001570_22_0_1; -.
DR InParanoid; Q9VRM7; -.
DR OMA; LRACANM; -.
DR OrthoDB; 702827at2759; -.
DR PhylomeDB; Q9VRM7; -.
DR BioGRID-ORCS; 38631; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 38631; -.
DR PRO; PR:Q9VRM7; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0003486; Expressed in yolk (Drosophila) and 8 other tissues.
DR ExpressionAtlas; Q9VRM7; baseline and differential.
DR Genevisible; Q9VRM7; DM.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:FlyBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; ISM:FlyBase.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; ISM:FlyBase.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0007417; P:central nervous system development; IMP:FlyBase.
DR GO; GO:0042335; P:cuticle development; IMP:FlyBase.
DR GO; GO:0007391; P:dorsal closure; IMP:FlyBase.
DR GO; GO:0006697; P:ecdysone biosynthetic process; IMP:FlyBase.
DR GO; GO:0001700; P:embryonic development via the syncytial blastoderm; IMP:FlyBase.
DR GO; GO:0048566; P:embryonic digestive tract development; IMP:FlyBase.
DR GO; GO:0008258; P:head involution; IMP:FlyBase.
DR GO; GO:0007494; P:midgut development; IMP:FlyBase.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Endoplasmic reticulum; Heme; Iron; Membrane;
KW Metal-binding; Microsome; Monooxygenase; Oxidoreductase; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..543
FT /note="Cytochrome P450 307a1"
FT /id="PRO_0000052316"
FT REGION 440..460
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 485
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT MOD_RES 219
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 543 AA; 62448 MW; F61DE137A0F5C219 CRC64;
MLAALIYTIL AILLSVLATS YICIIYGVKR RVLQPVKTKN STEINHNAYQ KYTQAPGPRP
WPIIGNLHLL DRYRDSPFAG FTALAQQYGD IYSLTFGHTR CLVVNNLELI REVLNQNGKV
MSGRPDFIRY HKLFGGERSN SLALCDWSQL QQKRRNLARR HCSPREFSCF YMKMSQIGCE
EMEHWNRELG NQLVPGEPIN IKPLILKACA NMFSQYMCSL RFDYDDVDFQ QIVQYFDEIF
WEINQGHPLD FLPWLYPFYQ RHLNKIINWS STIRGFIMER IIRHRELSVD LDEPDRDFTD
ALLKSLLEDK DVSRNTIIFM LEDFIGGHSA VGNLVMLVLA YIAKNVDIGR RIQEEIDAII
EEENRSINLL DMNAMPYTMA TIFEVLRYSS SPIVPHVATE DTVISGYGVT KGTIVFINNY
VLNTSEKFWV NPKEFNPLRF LEPSKEQSPK NSKGSDSGIE SDNEKLQLKR NIPHFLPFSI
GKRTCIGQNL VRGFGFLVVV NVMQRYNISS HNPSTIKISP ESLALPADCF PLVLTPREKI
GPL
