CP312_DROME
ID CP312_DROME Reviewed; 510 AA.
AC Q9VVN6; Q1ECB7;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Probable cytochrome P450 312a1;
DE EC=1.14.-.-;
DE AltName: Full=CYPCCCXIIA1;
GN Name=Cyp312a1; ORFNames=CG5137;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May be involved in the metabolism of insect hormones and in
CC the breakdown of synthetic insecticides. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AE014296; AAF49275.1; -; Genomic_DNA.
DR EMBL; BT025817; ABF85717.1; -; mRNA.
DR RefSeq; NP_001262008.1; NM_001275079.1.
DR RefSeq; NP_649030.1; NM_140773.3.
DR AlphaFoldDB; Q9VVN6; -.
DR SMR; Q9VVN6; -.
DR BioGRID; 65290; 2.
DR DIP; DIP-19123N; -.
DR IntAct; Q9VVN6; 1.
DR STRING; 7227.FBpp0305085; -.
DR PaxDb; Q9VVN6; -.
DR DNASU; 40005; -.
DR EnsemblMetazoa; FBtr0075135; FBpp0074901; FBgn0036778.
DR EnsemblMetazoa; FBtr0332863; FBpp0305085; FBgn0036778.
DR GeneID; 40005; -.
DR KEGG; dme:Dmel_CG5137; -.
DR UCSC; CG5137-RA; d. melanogaster.
DR CTD; 40005; -.
DR FlyBase; FBgn0036778; Cyp312a1.
DR VEuPathDB; VectorBase:FBgn0036778; -.
DR eggNOG; KOG0157; Eukaryota.
DR GeneTree; ENSGT00940000165700; -.
DR HOGENOM; CLU_001570_5_1_1; -.
DR InParanoid; Q9VVN6; -.
DR OMA; FENWLCE; -.
DR OrthoDB; 825914at2759; -.
DR PhylomeDB; Q9VVN6; -.
DR Reactome; R-DME-193144; Estrogen biosynthesis.
DR Reactome; R-DME-211976; Endogenous sterols.
DR SignaLink; Q9VVN6; -.
DR BioGRID-ORCS; 40005; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40005; -.
DR PRO; PR:Q9VVN6; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036778; Expressed in spermathecum and 12 other tissues.
DR ExpressionAtlas; Q9VVN6; baseline and differential.
DR Genevisible; Q9VVN6; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00465; EP450IV.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..510
FT /note="Probable cytochrome P450 312a1"
FT /id="PRO_0000052322"
FT BINDING 455
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 510 AA; 58805 MW; 6973570BF911E4E0 CRC64;
MFWLGFGLLL LALSLYLLYV FERQSRIDRL THKWPAPPAL PFIGHLHILA KLVGPHPLRR
ATEMINEHLH DHRAKLWMGT KLYLVDCNPK DIQALCSAQQ LLQKTNDYRV FENWLCEGLF
TSGFEKWSHR RKIVMPAFNY TMIKQFVAVF EKQSRILLTN VAKFAESGDQ IDFLQLISCF
TLDTICETAL GVSVGSQSSA KSEYLDAVKS ILVIIDKRLK NIFYRNSFIF KRTSHYKREQ
ELIKTLHGFT EGIIQKRIDE INQDAENRNY QSSDAELDGV KRTLCFLDTL LLSKGPDGKP
LTVKDIREEV DTIIFGGFDL TATTLNFFMY NMTLHPEHQQ RCREEVWSVC GKDKSEPISI
EQVRQLEFLE ACIKETLRMY PSGPLTARKA TANCTINDFF IPKGSDVIIS PIYMGRCKDF
FPDPMVFKPD RWAIGAEPKI EATTFIPFMA GARSCMGQRY AMVMLKMVLA HLLRNFLFEP
LGERQVKLKL NFVITLHTVE PYLCRAKNLD
