CP318_DROME
ID CP318_DROME Reviewed; 536 AA.
AC Q9VYQ5; Q8MZ48; Q9VYQ6;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2004, sequence version 4.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Probable cytochrome P450 318a1;
DE EC=1.14.-.-;
DE AltName: Full=CYPCCCXVIIIA1;
GN Name=Cyp318a1; ORFNames=CG1786;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Ovary;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: May be involved in the metabolism of insect hormones and in
CC the breakdown of synthetic insecticides. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AE014298; AAF48136.3; -; Genomic_DNA.
DR EMBL; AY113358; AAM29363.1; -; mRNA.
DR RefSeq; NP_727590.1; NM_167318.1.
DR AlphaFoldDB; Q9VYQ5; -.
DR SMR; Q9VYQ5; -.
DR IntAct; Q9VYQ5; 2.
DR STRING; 7227.FBpp0073460; -.
DR PaxDb; Q9VYQ5; -.
DR PRIDE; Q9VYQ5; -.
DR DNASU; 32172; -.
DR EnsemblMetazoa; FBtr0073623; FBpp0073460; FBgn0030369.
DR GeneID; 32172; -.
DR KEGG; dme:Dmel_CG1786; -.
DR UCSC; CG1786-RA; d. melanogaster.
DR CTD; 32172; -.
DR FlyBase; FBgn0030369; Cyp318a1.
DR VEuPathDB; VectorBase:FBgn0030369; -.
DR eggNOG; KOG0157; Eukaryota.
DR GeneTree; ENSGT00940000167150; -.
DR HOGENOM; CLU_001570_5_1_1; -.
DR InParanoid; Q9VYQ5; -.
DR OMA; KPWLQIR; -.
DR OrthoDB; 1247045at2759; -.
DR PhylomeDB; Q9VYQ5; -.
DR BioGRID-ORCS; 32172; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 32172; -.
DR PRO; PR:Q9VYQ5; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0030369; Expressed in mouthpart and 11 other tissues.
DR Genevisible; Q9VYQ5; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00465; EP450IV.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..536
FT /note="Probable cytochrome P450 318a1"
FT /id="PRO_0000052331"
FT REGION 439..460
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 477
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 536 AA; 61782 MW; 5FF17883A9E24BA9 CRC64;
MHLNIALWAC GALLAVLLAW QQRKCWRLIW QLNGWRGVIQ QPVLWLLLCI NLHPNSILEK
VSQYRVHFQR PLAVLVGTRV LLYIDDPAGM ECVLNAPECL DKTFLQDGFF VRRGLLHARG
QKWKLRRKQL NPAFSHNIVA SFFDVFNSVG NQMVEQFQTQ TNLHGQAVKF TAAEDLLSRA
VLEVSCLTIM GTPTNFTQLD DAHIAHSYKR LLEISAVRVV KPWLQIRLLH RLLAPELYEE
SKKCAKLLED FVGGIVRTKH RNWRLRDAVG GEKSGEDASN GWQRRIFIEQ IFQLAANGEM
TLEEIMDEAQ SMVLVSFETV SNSIMLALLC LATNKGDCQR RLLAEIRALV PDVGQVGLEQ
LQQLRYLDAF VSESLRLLAT VPMNLRHVSR DFRLAGRQHE TIVPQNSIVV LDTFNMQRDE
RWWGANARQF DPQRFLDQEE EQLSKGHNDS GSGEKRRQRD RRHSYSFLPF SNGLRSCIGR
RYGLFIMKVF LVKLITNFDF QSDFELEKLQ FVENISLKFK NADDILLTIQ PKKEST
