CP31B_ARATH
ID CP31B_ARATH Reviewed; 289 AA.
AC Q9FGS0;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 140.
DE RecName: Full=RNA-binding protein CP31B, chloroplastic {ECO:0000305};
DE Flags: Precursor;
GN Name=CP31B {ECO:0000303|PubMed:19297624};
GN OrderedLocusNames=At5g50250 {ECO:0000312|Araport:AT5G50250};
GN ORFNames=K6A12.11 {ECO:0000312|EMBL:BAB09396.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND
RP ADP-RIBOSYLATION.
RX PubMed=17450127; DOI=10.1038/nature05737;
RA Fu Z.Q., Guo M., Jeong B.R., Tian F., Elthon T.E., Cerny R.L., Staiger D.,
RA Alfano J.R.;
RT "A type III effector ADP-ribosylates RNA-binding proteins and quells plant
RT immunity.";
RL Nature 447:284-288(2007).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19297624; DOI=10.1073/pnas.0808529106;
RA Tillich M., Hardel S.L., Kupsch C., Armbruster U., Delannoy E.,
RA Gualberto J.M., Lehwark P., Leister D., Small I.D., Schmitz-Linneweber C.;
RT "Chloroplast ribonucleoprotein CP31A is required for editing and stability
RT of specific chloroplast mRNAs.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:6002-6007(2009).
CC -!- FUNCTION: Required for specific RNA editing events in chloroplasts and
CC stabilizes specific chloroplast mRNAs (PubMed:19297624).
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:17450127}.
CC -!- PTM: ADP-ribosylated by the Pseudomonas syringae type III effector
CC HopU1. ADP-ribosylation reduces the ability of the protein to bind RNA.
CC {ECO:0000269|PubMed:17450127}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant plants exhibit multiple specific editing defects
CC in chloroplast transcripts. {ECO:0000269|PubMed:19297624}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB024031; BAB09396.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95918.1; -; Genomic_DNA.
DR EMBL; AY039868; AAK63972.1; -; mRNA.
DR EMBL; AY077660; AAL76138.1; -; mRNA.
DR RefSeq; NP_199836.1; NM_124404.4.
DR AlphaFoldDB; Q9FGS0; -.
DR SMR; Q9FGS0; -.
DR STRING; 3702.AT5G50250.1; -.
DR PaxDb; Q9FGS0; -.
DR PRIDE; Q9FGS0; -.
DR ProteomicsDB; 220440; -.
DR EnsemblPlants; AT5G50250.1; AT5G50250.1; AT5G50250.
DR GeneID; 835090; -.
DR Gramene; AT5G50250.1; AT5G50250.1; AT5G50250.
DR KEGG; ath:AT5G50250; -.
DR Araport; AT5G50250; -.
DR TAIR; locus:2157702; AT5G50250.
DR eggNOG; KOG0118; Eukaryota.
DR HOGENOM; CLU_012062_15_1_1; -.
DR InParanoid; Q9FGS0; -.
DR OMA; MANASEW; -.
DR OrthoDB; 1202220at2759; -.
DR PhylomeDB; Q9FGS0; -.
DR PRO; PR:Q9FGS0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FGS0; baseline and differential.
DR Genevisible; Q9FGS0; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0008266; F:poly(U) RNA binding; IDA:TAIR.
DR GO; GO:1901259; P:chloroplast rRNA processing; IBA:GO_Central.
DR GO; GO:0045087; P:innate immune response; IDA:TAIR.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0009451; P:RNA modification; IMP:TAIR.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 2.
PE 1: Evidence at protein level;
KW Chloroplast; mRNA processing; Plastid; Reference proteome; Repeat;
KW Ribonucleoprotein; RNA editing; RNA-binding; Transit peptide.
FT TRANSIT 1..71
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 72..289
FT /note="RNA-binding protein CP31B, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431491"
FT DOMAIN 113..191
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 207..285
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
SQ SEQUENCE 289 AA; 31869 MW; 1470FB31564257EF CRC64;
MTSSVLTPSL KLLAMTNSSS STLFCIPSIF NISSSESHRF NFSLSSRPVN LTLSLKSKTL
RNSSPVVTFV SQTSNWAEEE EGEDGSIGGT SVTVDESFES EDGVGFPEPP EEAKLFVGNL
PYDVDSQALA MLFEQAGTVE ISEVIYNRDT DQSRGFGFVT MSTVEEAEKA VEKFNSFEVN
GRRLTVNRAA PRGSRPERQP RVYDAAFRIY VGNLPWDVDS GRLERLFSEH GKVVDARVVS
DRETGRSRGF GFVQMSNENE VNVAIAALDG QNLEGRAIKV NVAEERTRR
