CP392_DROME
ID CP392_DROME Reviewed; 538 AA.
AC P82713; Q8T422; Q9VQD3;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 19-SEP-2003, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Probable cytochrome P450 309a2;
DE EC=1.14.-.-;
DE AltName: Full=CYPCCCIXA2;
GN Name=Cyp309a2; ORFNames=CG18559;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Testis;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: May be involved in the metabolism of insect hormones and in
CC the breakdown of synthetic insecticides. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AE014134; AAF51242.3; -; Genomic_DNA.
DR EMBL; AY089391; AAL90129.1; -; mRNA.
DR RefSeq; NP_608689.2; NM_134845.2.
DR AlphaFoldDB; P82713; -.
DR SMR; P82713; -.
DR BioGRID; 59666; 1.
DR STRING; 7227.FBpp0077428; -.
DR PaxDb; P82713; -.
DR EnsemblMetazoa; FBtr0077748; FBpp0077428; FBgn0041337.
DR GeneID; 33439; -.
DR KEGG; dme:Dmel_CG18559; -.
DR UCSC; CG18559-RA; d. melanogaster.
DR CTD; 33439; -.
DR FlyBase; FBgn0041337; Cyp309a2.
DR VEuPathDB; VectorBase:FBgn0041337; -.
DR eggNOG; KOG0158; Eukaryota.
DR GeneTree; ENSGT00940000167276; -.
DR HOGENOM; CLU_001570_5_2_1; -.
DR InParanoid; P82713; -.
DR OMA; VLPIYLY; -.
DR OrthoDB; 1247045at2759; -.
DR PhylomeDB; P82713; -.
DR BioGRID-ORCS; 33439; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 33439; -.
DR PRO; PR:P82713; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0041337; Expressed in testis and 9 other tissues.
DR Genevisible; P82713; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..538
FT /note="Probable cytochrome P450 309a2"
FT /id="PRO_0000052319"
FT BINDING 483
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT CONFLICT 130
FT /note="S -> G (in Ref. 3; AAL90129)"
FT /evidence="ECO:0000305"
FT CONFLICT 240
FT /note="M -> L (in Ref. 3; AAL90129)"
FT /evidence="ECO:0000305"
FT CONFLICT 248
FT /note="E -> Q (in Ref. 3; AAL90129)"
FT /evidence="ECO:0000305"
FT CONFLICT 373
FT /note="C -> S (in Ref. 3; AAL90129)"
FT /evidence="ECO:0000305"
FT CONFLICT 419
FT /note="R -> K (in Ref. 3; AAL90129)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 538 AA; 62375 MW; B9F459671C8C6DB7 CRC64;
MMPERFSFDF RPIDQYWTRA KGACSNTKRG NMYILASLAL ILLHLLVLPI YLYLTWHHKY
WRKRGLVTAR PLTLLGTYPG LLTRKSNLVF DVQKIYDKYK GKHRAVGVFV TRQPQILVLD
PELAHEVLVS NFRCYKDSLQ SSYLRHAKWD KYARLNPFWA SGQSWRRLRT DAQAGISGSR
LRQAYNIWEQ GGQMLTEYMT QQVAEKNNIL ETRDLCFRYT AHVMADFIWG IDAGTLTRPM
EQPNKVQEMA SKWTSYAFYM LTLFMATIVA PCSRLLLRFR FYPKETDEFF SNLTKESIEL
RLKAGDSTRT DYLSHLLQLR DQKQATHDDL VGHALTVMLD GYDTSGTALL HALYYLAENP
AVQQKLRVEI LSCMASEKSL DFEKLSSLQY LEQVIYESLR LSSLIPQYTK VCTLPTVIRL
SESKSLDVEV GMTIMIPNYQ FHHDKQYFPE PEAFKPERFD NGAYQELMRK GIFLPFSDGP
RICMGVPLAM LTLKSALVHI LSNFQVVRGR DRLIPKGDSG FGVVLQGDVN LEYRRFFR
