ID   2SS1_BRAOA              Reviewed;          17 AA.
AC   P84782;
DT   07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 21.
DE   RecName: Full=Napin-like polypeptide;
DE   Contains:
DE     RecName: Full=Napin-like polypeptide small chain;
DE   Contains:
DE     RecName: Full=Napin-like polypeptide large chain;
DE   Flags: Fragments;
OS   Brassica oleracea var. alboglabra (Chinese kale) (Brassica alboglabra).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3714;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, AND FUNCTION.
RC   STRAIN=cv. Swatow {ECO:0000269|PubMed:15485558};
RC   TISSUE=Seed {ECO:0000269|PubMed:15485558};
RX   PubMed=15485558; DOI=10.1111/j.1399-3011.2004.00186.x;
RA   Ngai P.H., Ng T.B.;
RT   "A napin-like polypeptide with translation-inhibitory, trypsin-inhibitory,
RT   antiproliferative and antibacterial activities from kale seeds.";
RL   J. Pept. Res. 64:202-208(2004).
CC   -!- FUNCTION: Inhibits cell-free protein synthesis. Inhibits trypsin and
CC       chymotrypsin. Does not inhibit ribonuclease of protease activity. Has
CC       antibacterial activity against Gram-negative bacterium P.fluorescens,
CC       and the Gram-positive bacteria B.subtilis, B.cereus, B.megaterium and
CC       M.phlei. No antibacterial activity against the Gram-positive bacterium
CC       S.aureus or the Gram-negative bacteria E.coli, E.aerogenes,
CC       P.aeruginosa and P.vulgaris. Inhibits the proliferation of leukemia
CC       cells in vitro. {ECO:0000269|PubMed:15485558}.
CC   -!- SUBUNIT: The mature protein consists of a small and a large chain
CC       linked by disulfide bonds. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P84782; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0008285; P:negative regulation of cell population proliferation; IDA:UniProtKB.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Disulfide bond; Protein synthesis inhibitor.
FT   CHAIN           1..>9
FT                   /note="Napin-like polypeptide small chain"
FT                   /evidence="ECO:0000269|PubMed:15485558"
FT                   /id="PRO_0000225612"
FT   CHAIN           10..>17
FT                   /note="Napin-like polypeptide large chain"
FT                   /evidence="ECO:0000269|PubMed:15485558"
FT                   /id="PRO_0000225613"
FT   NON_CONS        9..10
FT                   /evidence="ECO:0000303|PubMed:15485558"
FT   NON_TER         17
FT                   /evidence="ECO:0000303|PubMed:15485558"
SQ   SEQUENCE   17 AA;  2052 MW;  1990F6731BF332C1 CRC64;
     PAQPFRIKKR QGPFERP