ID   CP422_SCHJA             Reviewed;         133 AA.
AC   Q5DFZ7; Q1A4H0; Q4JL30; Q6PYV0;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   29-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Egg protein CP422;
DE   AltName: Full=Protease inhibitor bi-domain;
DE   Flags: Precursor;
GN   Name=CP422;
OS   Schistosoma japonicum (Blood fluke).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes; Trematoda;
OC   Digenea; Strigeidida; Schistosomatoidea; Schistosomatidae; Schistosoma.
OX   NCBI_TaxID=6182;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=16617374; DOI=10.1371/journal.ppat.0020029;
RA   Liu F., Lu J., Hu W., Wang S.-Y., Cui S.-J., Chi M., Yan Q., Wang X.-R.,
RA   Song H.-D., Xu X.-N., Wang J.-J., Zhang X.-L., Zhang X., Wang Z.-Q.,
RA   Xue C.-L., Brindley P.J., McManus D.P., Yang P.-Y., Feng Z., Chen Z.,
RA   Han Z.-G.;
RT   "New perspectives on host-parasite interplay by comparative transcriptomic
RT   and proteomic analyses of Schistosoma japonicum.";
RL   PLoS Pathog. 2:268-281(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA   Yu C., Zhang F., Mihoko K., Masahiro I., Zhu Y., Hirayama K.;
RT   "Using retrovirus SST system to isolate the cDNA harboring functional
RT   signal sequence from egg of Schistosoma japonicum (Chinese Mainland
RT   Strain).";
RL   Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC   STRAIN=Chinese;
RA   Yuan C.-X., Lu K., Lin J.-J.;
RT   "Cloning and function prediction of Schistosoma japonicum differentially
RT   expressed protein.";
RL   Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
RA   Zhu S., Gao B.;
RT   "Genomic DNA encoding an inhibitor cystine knot (ICK) peptide, a putative
RT   protease inhibitor bi-domain.";
RL   Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q5DFZ7-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q5DFZ7-2; Sequence=VSP_029562;
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
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DR   EMBL; AY813527; AAW25259.1; -; mRNA.
DR   EMBL; AY570752; AAS68257.1; -; mRNA.
DR   EMBL; DQ076155; AAY97893.1; -; mRNA.
DR   EMBL; DQ335568; ABC61058.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5DFZ7; -.
DR   PRIDE; Q5DFZ7; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Disulfide bond; Knottin; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..133
FT                   /note="Egg protein CP422"
FT                   /id="PRO_0000311405"
FT   DISULFID        107..121
FT                   /evidence="ECO:0000305"
FT   DISULFID        114..125
FT                   /evidence="ECO:0000305"
FT   DISULFID        120..130
FT                   /evidence="ECO:0000305"
FT   VAR_SEQ         21..61
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2, ECO:0000303|Ref.3"
FT                   /id="VSP_029562"
FT   CONFLICT        2
FT                   /note="H -> R (in Ref. 4; ABC61058)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        63
FT                   /note="E -> D (in Ref. 3; AAY97893)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        106
FT                   /note="G -> S (in Ref. 4; ABC61058)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   133 AA;  14858 MW;  3A1997AB87729F3E CRC64;
     MHECMIVFFI FAVVSIYYAD AESCLYLGQH CNALATKKCC PPFTCKFVNQ REGICVREDP
     GAESCLYLGQ HCNALATKKC CPPFTCKFVN QREGICVRED PGVGGGCLPD KQQCRRNKDC
     CSKSCYRGNC RSK