CP4B1_MOUSE
ID CP4B1_MOUSE Reviewed; 511 AA.
AC Q64462;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Cytochrome P450 4B1;
DE EC=1.14.14.1;
DE AltName: Full=CYPIVB1;
GN Name=Cyp4b1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=BALB/cJ; TISSUE=Kidney;
RX PubMed=7639529; DOI=10.1006/abbi.1995.1393;
RA Imaoka S., Hiroi T., Tamura Y., Yamazaki H., Shimada T., Komori M.,
RA Degawa M., Funae Y.;
RT "Mutagenic activation of 3-methoxy-4-aminoazobenzene by mouse renal
RT cytochrome P450 CYP4B1: cloning and characterization of mouse CYP4B1.";
RL Arch. Biochem. Biophys. 321:255-262(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, Kidney, and Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Responsible for mutagenic activation of 3-methoxy-4-
CC aminoazobenzene (3-MeO-AAB); a potent procarcinogen. Also active on 2-
CC aminofluorene and 2-aminoanthracene.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an organic molecule + O2 + reduced [NADPH--hemoprotein
CC reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein
CC reductase]; Xref=Rhea:RHEA:17149, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:30879, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC ChEBI:CHEBI:142491; EC=1.14.14.1;
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000250|UniProtKB:P51869};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral
CC membrane protein. Microsome membrane; Peripheral membrane protein.
CC -!- TISSUE SPECIFICITY: Present abundantly in renal microsomes of male mice
CC but not in those of female mice. Also present in pulmonary microsomes
CC of male and female mice. Not found in liver.
CC -!- INDUCTION: P450 can be induced to high levels in liver and other
CC tissues by various foreign compounds, including drugs, pesticides, and
CC carcinogens.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; D50834; BAA09446.1; -; mRNA.
DR EMBL; BC008996; AAH08996.1; -; mRNA.
DR CCDS; CCDS18493.1; -.
DR PIR; S66472; S66472.
DR RefSeq; NP_031849.1; NM_007823.2.
DR AlphaFoldDB; Q64462; -.
DR SMR; Q64462; -.
DR BioGRID; 199036; 24.
DR STRING; 10090.ENSMUSP00000099768; -.
DR iPTMnet; Q64462; -.
DR PhosphoSitePlus; Q64462; -.
DR jPOST; Q64462; -.
DR MaxQB; Q64462; -.
DR PaxDb; Q64462; -.
DR PRIDE; Q64462; -.
DR ProteomicsDB; 284153; -.
DR Antibodypedia; 981; 166 antibodies from 29 providers.
DR DNASU; 13120; -.
DR Ensembl; ENSMUST00000102707; ENSMUSP00000099768; ENSMUSG00000028713.
DR GeneID; 13120; -.
DR KEGG; mmu:13120; -.
DR UCSC; uc008ufc.1; mouse.
DR CTD; 1580; -.
DR MGI; MGI:103225; Cyp4b1.
DR VEuPathDB; HostDB:ENSMUSG00000028713; -.
DR eggNOG; KOG0157; Eukaryota.
DR GeneTree; ENSGT00940000161441; -.
DR InParanoid; Q64462; -.
DR OMA; LFGHAQE; -.
DR OrthoDB; 1247045at2759; -.
DR PhylomeDB; Q64462; -.
DR TreeFam; TF105088; -.
DR Reactome; R-MMU-211935; Fatty acids.
DR Reactome; R-MMU-211958; Miscellaneous substrates.
DR Reactome; R-MMU-211979; Eicosanoids.
DR Reactome; R-MMU-2142691; Synthesis of Leukotrienes (LT) and Eoxins (EX).
DR BioGRID-ORCS; 13120; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Cyp4b1; mouse.
DR PRO; PR:Q64462; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q64462; protein.
DR Bgee; ENSMUSG00000028713; Expressed in right kidney and 125 other tissues.
DR ExpressionAtlas; Q64462; baseline and differential.
DR Genevisible; Q64462; MM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0070330; F:aromatase activity; IEA:UniProtKB-EC.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:1901363; F:heterocyclic compound binding; ISO:MGI.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0018879; P:biphenyl metabolic process; ISO:MGI.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Endoplasmic reticulum; Heme; Iron; Membrane;
KW Metal-binding; Microsome; Monooxygenase; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..511
FT /note="Cytochrome P450 4B1"
FT /id="PRO_0000051820"
FT BINDING 315
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /note="covalent"
FT /evidence="ECO:0000250|UniProtKB:P51869"
FT BINDING 453
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250|UniProtKB:P51869"
SQ SEQUENCE 511 AA; 58900 MW; 35463ACDFFE6328B CRC64;
MALSFLSPSL SRLGLWASVV ILMVTVLKLL SLLFRRQKLA RALDSFPGPP KHWLFGHALE
IQKTGGLDKV VTWTEQFPYA HPLWLGQFIV FLNIYEPDYA KAVYSRGDPK AAYVYDFFLQ
WIGKGLLVLE GPKWFQHRKL LTPGFHYDVL KPYVAIFAES TRVMLDKWEK KASENKSFDI
FCDVGHMALD TLMKCTFGKG DSGLSHSDNS YYLAVSDLTL LMQQRIDSFQ YHNDFIYWLT
PHGRRFLRAC QIAHDHTDHV IRQRKAALQD EKEQKKLQER RHLDFLDILL GARDESGIKL
SDADLRAEVD TFMFEGHDTT TSGISWFLYC MALYPMHQQR CREEVREILG DRDSFQWDDL
AQMTYLTMCM KECFRLYPPV PQVYRQLSKP VTFVDGRSLP AGSLISLHIY ALHRNSAVWP
DPEVFDPLRF SPENMTGRHP FAFMPFSAGP RNCIGQQFAM NEMKVVTALC LLRFEFSPDP
SKIPIKVPQL ILRSKNGIHL YLKPLGPGSG K
