ID   CP4CU_BLAGE             Reviewed;         501 AA.
AC   Q964T1;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Cytochrome P450 4c21;
DE            EC=1.14.14.1;
DE   AltName: Full=CYPIVC21;
GN   Name=CYP4C21;
OS   Blattella germanica (German cockroach) (Blatta germanica).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Polyneoptera; Dictyoptera; Blattodea; Blaberoidea; Ectobiidae;
OC   Blattellinae; Blattella.
OX   NCBI_TaxID=6973;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RX   PubMed=11470532; DOI=10.1016/s0378-1119(01)00529-7;
RA   Wen Z., Horak C.E., Scott J.G.;
RT   "CYP9E2, CYP4C21 and related pseudogenes from German cockroaches, Blattella
RT   germanica: implications for molecular evolution, expression studies and
RT   nomenclature of P450s.";
RL   Gene 272:257-266(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an organic molecule + O2 + reduced [NADPH--hemoprotein
CC         reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein
CC         reductase]; Xref=Rhea:RHEA:17149, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC         COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:30879, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:142491; EC=1.14.14.1;
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC       Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC       {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC   -!- INDUCTION: Expressed at all life stages. {ECO:0000269|PubMed:11470532}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF275641; AAK69411.1; -; mRNA.
DR   AlphaFoldDB; Q964T1; -.
DR   SMR; Q964T1; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070330; F:aromatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW   Monooxygenase; Oxidoreductase.
FT   CHAIN           1..501
FT                   /note="Cytochrome P450 4c21"
FT                   /id="PRO_0000051824"
FT   BINDING         309
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /note="covalent"
FT                   /evidence="ECO:0000250"
FT   BINDING         447
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   501 AA;  58297 MW;  825F90FAA12AB22B CRC64;
     MDITVFLSLT VVVFLAVIIF YGRNEERKRM LQKIPGAPSL PLIGTALPIL YRRKEDRMTW
     IEEILEKYKP LILWYFGNRP FVNISSPELI EVVLRNTQLI DKAFLYDLFH SWLGTGLLTS
     SGAKWHQHRK IITPTFHFSI LEGFITIFAE KSEILVRKLQ KEVGRGPFFI RQYVSNCALD
     IICETAMGTS VNAQDEGFSE YVTAINKMTD VLSDRMANPL LYPEFIFKLT PYYWTHKKCL
     KVLNGFVNKI IQERKEERKK SKVTQTSEDA DIGKKKRVPF LDTLLDASED DNKLTDTDIL
     EEVHTFMFEG HDTVSAAMTW LLFELGHHPE IQEEAYKEVQ DIFQGSDRVP TMADLNNMNY
     LERVIKESLR LHPSVIYFVR EAHQDFELGG YTIPAGTNID FSVPFIHRNP EIFPNPRCFN
     PDNFLPDRVV NRHPYAYIPF SAGPRNCIGQ RFALLEEKVV LSYLLRHYRF RTVNKREDSK
     FKLEMINTPV KPIQLIIEAR N