CP4D2_DROME
ID CP4D2_DROME Reviewed; 501 AA.
AC Q27589; O18651; O18674; O46053; Q27588; Q9W514;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Cytochrome P450 4d2;
DE EC=1.14.-.-;
DE AltName: Full=CYPIVD2;
GN Name=Cyp4d2; ORFNames=CG3466;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=8024706; DOI=10.1089/dna.1994.13.663;
RA Frolov M.V., Alatortsev V.E.;
RT "Cluster of cytochrome P450 genes on the X chromosome of Drosophila
RT melanogaster.";
RL DNA Cell Biol. 13:663-668(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 4-501.
RC STRAIN=CAM-1, CAM-12, CAM-14, CAM-19, CAM-2, CAM-3, CAM-38, CAM-41, CAM-42,
RC CAM-44, CAM-48, CAM-8, and CAM-9;
RA Phillips K.S., Begun D.J., Aquadro C.F.;
RT "Evidence for non-neutral evolution around the cytochrome p450 gene cluster
RT on the Drosophila melanogaster X chromosome.";
RL Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the metabolism of insect hormones and in the
CC breakdown of synthetic insecticides. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; X75955; CAA53568.1; -; Genomic_DNA.
DR EMBL; Z23005; CAA80549.1; -; Genomic_DNA.
DR EMBL; AE014298; AAF45741.1; -; Genomic_DNA.
DR EMBL; AL009194; CAA15698.1; -; Genomic_DNA.
DR EMBL; AY118763; AAM50623.1; -; mRNA.
DR EMBL; AF017006; AAB71169.1; -; Genomic_DNA.
DR EMBL; AF017007; AAB71170.1; -; Genomic_DNA.
DR EMBL; AF017008; AAB71171.1; -; Genomic_DNA.
DR EMBL; AF017009; AAB71172.1; -; Genomic_DNA.
DR EMBL; AF017010; AAB71173.1; -; Genomic_DNA.
DR EMBL; AF017011; AAB71174.1; -; Genomic_DNA.
DR EMBL; AF017012; AAB71175.1; -; Genomic_DNA.
DR EMBL; AF017013; AAB71176.1; -; Genomic_DNA.
DR EMBL; AF017014; AAB71177.1; -; Genomic_DNA.
DR EMBL; AF017015; AAB71178.1; -; Genomic_DNA.
DR EMBL; AF017016; AAB71179.1; -; Genomic_DNA.
DR EMBL; AF017017; AAB71180.1; -; Genomic_DNA.
DR EMBL; AF017018; AAB71181.1; -; Genomic_DNA.
DR PIR; S41192; S41192.
DR RefSeq; NP_001284806.1; NM_001297877.1.
DR RefSeq; NP_525043.1; NM_080304.3.
DR AlphaFoldDB; Q27589; -.
DR SMR; Q27589; -.
DR IntAct; Q27589; 1.
DR STRING; 7227.FBpp0070371; -.
DR PaxDb; Q27589; -.
DR DNASU; 31192; -.
DR GeneID; 31192; -.
DR KEGG; dme:Dmel_CG3466; -.
DR CTD; 31192; -.
DR FlyBase; FBgn0011576; Cyp4d2.
DR VEuPathDB; VectorBase:FBgn0011576; -.
DR eggNOG; KOG0157; Eukaryota.
DR HOGENOM; CLU_001570_5_1_1; -.
DR InParanoid; Q27589; -.
DR PhylomeDB; Q27589; -.
DR Reactome; R-DME-193144; Estrogen biosynthesis.
DR Reactome; R-DME-211976; Endogenous sterols.
DR Reactome; R-DME-2453902; The canonical retinoid cycle in rods (twilight vision).
DR SignaLink; Q27589; -.
DR BioGRID-ORCS; 31192; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 31192; -.
DR PRO; PR:Q27589; -.
DR Proteomes; UP000000803; Chromosome X.
DR ExpressionAtlas; Q27589; differential.
DR Genevisible; Q27589; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..501
FT /note="Cytochrome P450 4d2"
FT /id="PRO_0000051834"
FT BINDING 311
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /note="covalent"
FT /evidence="ECO:0000250"
FT BINDING 449
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT VARIANT 163
FT /note="K -> M (in strain: CAM-44, CAM-48 and Berkeley)"
FT CONFLICT 2..25
FT /note="Missing (in Ref. 2; CAA15698)"
FT /evidence="ECO:0000305"
FT CONFLICT 30
FT /note="I -> A (in Ref. 1; CAA80549)"
FT /evidence="ECO:0000305"
FT CONFLICT 160
FT /note="A -> R (in Ref. 1; CAA53568/CAA80549)"
FT /evidence="ECO:0000305"
FT CONFLICT 487..500
FT /note="LRSANGVHLGLKPR -> CGRPTAFIL (in Ref. 1; CAA53568/
FT CAA80549)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 501 AA; 57914 MW; 8F8D98DBF39FD1A2 CRC64;
MLGVVGVLLL VAFATLLLWD FLWRRRGNGI LPGPRPLPFL GNLLMYRGLD PEQIMDFVKK
NQRKYGRLYR VWILHQLAVF STDPRDIEFV LSSQQHITKN NLYKLLNCWL GDGLLMSTGR
KWHGRRKIIT PTFHFKILEQ FVEIFDQQSA VMVEQLQSRA DGKTPINIFP VICLTALDII
AETAMGTKIN AQKNPNLPYV QAVNDVTNIL IKRFIHAWQR VDWIFRLTQP TEAKRQDKAI
KVMHDFTENI IRERRETLVN NSKETTPEEE VNFLGQKRRM ALLDVLLQST IDGAPLSDED
IREEVDTFMF EGHDTTTSAI SFCLYEISRH PEVQQRLQQE IRDVLGEDRK SPVTLRDLGE
LKFMENVIKE SLRLHPPVPM IGRWFAEDVE IRGKHIPAGT NFTMGIFVLL RDPEYFESPD
EFRPERFDAD VPQIHPYAYI PFSAGPRNCI GQKFAMLEMK STVSKLLRHF ELLPLGPEPR
HSMNIVLRSA NGVHLGLKPR A
