CP4E3_DROME
ID CP4E3_DROME Reviewed; 526 AA.
AC Q9VL92; Q24128;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Cytochrome P450 4e3;
DE EC=1.14.-.-;
DE AltName: Full=CYPIVE3;
GN Name=Cyp4e3; ORFNames=CG4105;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE OF 312-433.
RC STRAIN=Haag-79;
RX PubMed=8676871; DOI=10.1007/bf02172519;
RA Dunkov B.C., Rodriguez-Arnaiz R., Pittendrigh B., ffrench-Constant R.H.,
RA Feyereisen R.;
RT "Cytochrome P450 gene clusters in Drosophila melanogaster.";
RL Mol. Gen. Genet. 251:290-297(1996).
CC -!- FUNCTION: May be involved in the metabolism of insect hormones and in
CC the breakdown of synthetic insecticides. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AE014134; AAF52804.1; -; Genomic_DNA.
DR EMBL; U34330; AAA80664.1; -; mRNA.
DR RefSeq; NP_523527.1; NM_078803.2.
DR AlphaFoldDB; Q9VL92; -.
DR SMR; Q9VL92; -.
DR STRING; 7227.FBpp0079515; -.
DR PaxDb; Q9VL92; -.
DR DNASU; 34291; -.
DR EnsemblMetazoa; FBtr0079925; FBpp0079515; FBgn0015035.
DR GeneID; 34291; -.
DR KEGG; dme:Dmel_CG4105; -.
DR CTD; 34291; -.
DR FlyBase; FBgn0015035; Cyp4e3.
DR VEuPathDB; VectorBase:FBgn0015035; -.
DR eggNOG; KOG0157; Eukaryota.
DR GeneTree; ENSGT00940000165700; -.
DR HOGENOM; CLU_001570_5_1_1; -.
DR InParanoid; Q9VL92; -.
DR OMA; GRHKYDP; -.
DR OrthoDB; 1247045at2759; -.
DR PhylomeDB; Q9VL92; -.
DR Reactome; R-DME-193144; Estrogen biosynthesis.
DR Reactome; R-DME-211976; Endogenous sterols.
DR BioGRID-ORCS; 34291; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 34291; -.
DR PRO; PR:Q9VL92; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0015035; Expressed in adult Malpighian tubule (Drosophila) and 17 other tissues.
DR ExpressionAtlas; Q9VL92; baseline and differential.
DR Genevisible; Q9VL92; DM.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:FlyBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..526
FT /note="Cytochrome P450 4e3"
FT /id="PRO_0000051842"
FT BINDING 307
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /note="covalent"
FT /evidence="ECO:0000250"
FT BINDING 443
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 526 AA; 60883 MW; 993BCEEAFCDFF6CC CRC64;
MWLAVLALLV LPLITLVYFE RKASQRRQLL KEFNGPTPVP ILGNANRIGK NPAEILSTFF
DWWYDYGKDN FLFWIGYSSH IVMTNPKQLE YILNSQQLIQ KSTIYDLLHP WLGHGLLTSF
GSKWHKHRKM ITPSFHFNIL QDFHEVMNEN SAKFMTQLKK ASAGDTIIDF QEHANYLTLD
VICDTAMGVP INAMEQRDSS IVQAFRDMCY NINMRAFHPF KRSNRVFSLT PEFSAYQKTL
KTLQDFTYDI IEKRVYALQN GGSKEDHDPS LPRKKMAFLD TLLSSTIDGR PLTRQEIYEE
VSTFMFEGHD TTTSGVSFSV YLLSRHPDVQ RKLYREQCEV MGHDMNRSVS FQEIAKMKYL
DLFIKEAQRV YPSVPFIGRY CDKDYDINGS IVPKGTTLNL ALILLGYNDR IFKDPHHFRP
ERFEEEKPAP FEYLPFSAGP RNCIGQKFAL LELKTVISKV VRSFEVLPAV DELVSTDGRL
NTYLGLAPDE KLKREAGRHK YDPILSAVLT LKSDNGLHLR LRERRS
