CP4P1_DROME
ID CP4P1_DROME Reviewed; 513 AA.
AC Q9V558; Q24125;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Cytochrome P450 4p1;
DE EC=1.14.-.-;
DE AltName: Full=CYPIVP1;
GN Name=Cyp4p1; ORFNames=CG10842;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE OF 325-450.
RC STRAIN=Oregon-R;
RX PubMed=8676871; DOI=10.1007/bf02172519;
RA Dunkov B.C., Rodriguez-Arnaiz R., Pittendrigh B., ffrench-Constant R.H.,
RA Feyereisen R.;
RT "Cytochrome P450 gene clusters in Drosophila melanogaster.";
RL Mol. Gen. Genet. 251:290-297(1996).
CC -!- FUNCTION: May be involved in the metabolism of insect hormones and in
CC the breakdown of synthetic insecticides. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE013599; AAF58962.1; -; Genomic_DNA.
DR EMBL; AY071584; AAL49206.1; -; mRNA.
DR EMBL; U34327; AAA80661.1; -; mRNA.
DR PIR; S70624; S70624.
DR RefSeq; NP_524828.1; NM_080089.3.
DR AlphaFoldDB; Q9V558; -.
DR SMR; Q9V558; -.
DR BioGRID; 69739; 1.
DR DIP; DIP-18909N; -.
DR IntAct; Q9V558; 3.
DR STRING; 7227.FBpp0087673; -.
DR PaxDb; Q9V558; -.
DR PRIDE; Q9V558; -.
DR EnsemblMetazoa; FBtr0088592; FBpp0087673; FBgn0015037.
DR GeneID; 45524; -.
DR KEGG; dme:Dmel_CG10842; -.
DR CTD; 45524; -.
DR FlyBase; FBgn0015037; Cyp4p1.
DR VEuPathDB; VectorBase:FBgn0015037; -.
DR eggNOG; KOG0157; Eukaryota.
DR GeneTree; ENSGT00940000167779; -.
DR HOGENOM; CLU_001570_5_1_1; -.
DR InParanoid; Q9V558; -.
DR OMA; HYYSWIN; -.
DR OrthoDB; 1247045at2759; -.
DR PhylomeDB; Q9V558; -.
DR Reactome; R-DME-193144; Estrogen biosynthesis.
DR Reactome; R-DME-211976; Endogenous sterols.
DR SignaLink; Q9V558; -.
DR BioGRID-ORCS; 45524; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 45524; -.
DR PRO; PR:Q9V558; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0015037; Expressed in adult Malpighian tubule (Drosophila) and 24 other tissues.
DR Genevisible; Q9V558; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..513
FT /note="Cytochrome P450 4p1"
FT /id="PRO_0000051845"
FT BINDING 320
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /note="covalent"
FT /evidence="ECO:0000250"
FT BINDING 459
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 513 AA; 59433 MW; F3D7500719BAF2C4 CRC64;
MIILWLILAL SALLYWLHRA NKDYHILSFF TKRIRLKDGT PVEIIAPIAK GKTIFGNTLD
LYGRDHAGVF NYSRERAKEM GTSYIEYVFG KAIYNIIDAD SAENVLNHPN LITKGLVYNF
LHPFLRTGLL TSTGKKWHAR RKMLTPTFHF NILNQFQEIF KTESQKFLLQ FEGQDEVTIT
LHDVIPRFTL NSICETAMGV KLDEMAEKGD RYRENFSQIE ECFIRRLSNP LLWGDKLFEM
FAAKDFASAL DVVHRFSSEI IAKRRDLLKD ELDKSSSTAD DDGFVSKKRF AMLDTLIYAE
KDGLIDHIGI CEEVDTLMFE GYDTTSIGLI FGLMNMSLNP DKQELCYQEI QEHIDDDLSN
LDVGQLNKLK YLEYFMKETT RLFPSVPIMG REAVQETELA NGLILPKGAQ ITIHVFDIHR
NAKYWDSPEE FRPERFLPEN VQDRHTYAYV PFSAGQRNCI GKKYAMQEMK TLMVVLLKQF
KVLKAIDPQK IVFHTGITLR TQDKIRVKLV RRT
