ID   2SS1_BRARC              Reviewed;          20 AA.
AC   P84529;
DT   24-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 24.
DE   RecName: Full=Antibacterial napin;
DE   Contains:
DE     RecName: Full=Napin small chain;
DE   Contains:
DE     RecName: Full=Napin large chain;
DE   Flags: Fragments;
OS   Brassica rapa subsp. chinensis (Pak-choi) (Brassica chinensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=93385;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, AND FUNCTION.
RC   TISSUE=Seed {ECO:0000269|PubMed:15062997};
RX   PubMed=15062997; DOI=10.1016/j.peptides.2003.12.012;
RA   Ngai P.H.K., Ng T.B.;
RT   "A napin-like polypeptide from dwarf Chinese white cabbage seeds with
RT   translation-inhibitory, trypsin-inhibitory, and antibacterial activities.";
RL   Peptides 25:171-176(2004).
CC   -!- FUNCTION: The small, basic, water-soluble napins are one of the two
CC       major kinds of storage proteins synthesized in the seed during its
CC       maturation. Inhibits cell-free protein synthesis. Has antibacterial
CC       activity against Gram-negative bacterium P.fluorescens, and the Gram-
CC       positive bacteria B.subtilis, B.cereus, B.megaterium and M.phei. No
CC       antibacterial activity against the Gram-positive bacterium S.aureus or
CC       the Gram-negative bacteria E.coli, E.aerogenes and P.aeruginosa.
CC       {ECO:0000269|PubMed:15062997}.
CC   -!- SUBUNIT: The mature protein consists of a small and a large chain
CC       linked by disulfide bonds. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P84529; -.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Protein synthesis inhibitor; Seed storage protein; Storage protein.
FT   CHAIN           1..>10
FT                   /note="Napin small chain"
FT                   /evidence="ECO:0000269|PubMed:15062997"
FT                   /id="PRO_0000032133"
FT   CHAIN           11..>20
FT                   /note="Napin large chain"
FT                   /evidence="ECO:0000269|PubMed:15062997"
FT                   /id="PRO_0000032134"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_CONS        10..11
FT                   /evidence="ECO:0000303|PubMed:15062997"
FT   NON_TER         20
FT                   /evidence="ECO:0000303|PubMed:15062997"
SQ   SEQUENCE   20 AA;  2297 MW;  F376FB58E8F05EA4 CRC64;
     PAQPFRFPKH PQGPQTRPPI