ID   CP56_YEAST              Reviewed;         489 AA.
AC   P21595; D6VT34;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   03-AUG-2022, entry version 170.
DE   RecName: Full=Cytochrome P450-DIT2;
DE            EC=1.14.14.-;
DE   AltName: Full=Cytochrome P450 56;
GN   Name=DIT2; Synonyms=CYP56; OrderedLocusNames=YDR402C; ORFNames=D9509.20;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204510 / AB320;
RX   PubMed=2249774; DOI=10.1101/gad.4.10.1775;
RA   Briza P., Breitenbach M., Ellinger A., Segall J.;
RT   "Isolation of two developmentally regulated genes involved in spore wall
RT   maturation in Saccharomyces cerevisiae.";
RL   Genes Dev. 4:1775-1789(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA   Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA   Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA   Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA   Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA   Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA   Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA   Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA   Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA   Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA   Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA   Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA   Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA   Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA   Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA   Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA   Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA   Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA   Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA   Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=8183942; DOI=10.1073/pnas.91.10.4524;
RA   Briza P., Eckerstorfer M., Breitenbach M.;
RT   "The sporulation-specific enzymes encoded by the DIT1 and DIT2 genes
RT   catalyze a two-step reaction leading to a soluble LL-dityrosine-containing
RT   precursor of the yeast spore wall.";
RL   Proc. Natl. Acad. Sci. U.S.A. 91:4524-4528(1994).
CC   -!- FUNCTION: Involved in spore wall maturation. Thought to catalyze the
CC       oxidation of tyrosine residues in the formation of LL-dityrosine a
CC       precursor of the spore wall.
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- DEVELOPMENTAL STAGE: Sporulation.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; X55713; CAA39246.1; -; Genomic_DNA.
DR   EMBL; U32274; AAB64842.1; -; Genomic_DNA.
DR   EMBL; BK006938; DAA12244.1; -; Genomic_DNA.
DR   PIR; B36395; B36395.
DR   RefSeq; NP_010690.1; NM_001180710.1.
DR   AlphaFoldDB; P21595; -.
DR   SMR; P21595; -.
DR   BioGRID; 32462; 170.
DR   STRING; 4932.YDR402C; -.
DR   PaxDb; P21595; -.
DR   PRIDE; P21595; -.
DR   EnsemblFungi; YDR402C_mRNA; YDR402C; YDR402C.
DR   GeneID; 852011; -.
DR   KEGG; sce:YDR402C; -.
DR   SGD; S000002810; DIT2.
DR   VEuPathDB; FungiDB:YDR402C; -.
DR   eggNOG; KOG0157; Eukaryota.
DR   GeneTree; ENSGT00940000167749; -.
DR   HOGENOM; CLU_031576_0_0_1; -.
DR   InParanoid; P21595; -.
DR   OMA; FFGSRWN; -.
DR   BioCyc; YEAST:G3O-29946-MON; -.
DR   PRO; PR:P21595; -.
DR   Proteomes; UP000002311; Chromosome IV.
DR   RNAct; P21595; protein.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003959; F:NADPH dehydrogenase activity; IDA:SGD.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0030476; P:ascospore wall assembly; IDA:SGD.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   1: Evidence at protein level;
KW   Heme; Iron; Metal-binding; Monooxygenase; Oxidoreductase;
KW   Reference proteome; Sporulation.
FT   CHAIN           1..489
FT                   /note="Cytochrome P450-DIT2"
FT                   /id="PRO_0000052042"
FT   BINDING         435
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        445
FT                   /note="M -> I (in Ref. 1; CAA39246)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   489 AA;  56071 MW;  9A301276DDBEB18F CRC64;
     MELLKLLCLI LFLTLSYVAF AIIVPPLNFP KNIPTIPFYV VFLPVIFPID QTELYDLYIR
     ESMEKYGAVK FFFGSRWNIL VSRSEYLAQI FKDEDTFAKS GNQKKIPYSA LAAYTGDNVI
     SAYGAVWRNY RNAVTNGLQH FDDAPIFKNA KILCTLIKNR LLEGQTSIPM GPLSQRMALD
     NISQVALGFD FGALTHEKNA FHEHLIRIKK QIFHPFFLTF PFLDVLPIPS RKKAFKDVVS
     FRELLVKRVQ DELVNNYKFE QTTFAASDLI RAHNNEIIDY KQLTDNIVII LVAGHENPQL
     LFNSSLYLLA KYSNEWQEKL RKEVNGITDP KGLADLPLLN AFLFEVVRMY PPLSTIINRC
     TTKTCKLGAE IVIPKGVYVG YNNFGTSHDP KTWGTTADDF KPERWGSDIE TIRKNWRMAK
     NRCAVTGFHG GRRACLGEKL ALTEMRISLA EMLKQFRWSL DPEWEEKLTP AGPLCPLNLK
     LKFNENIME