ID   CP67_UROFA              Reviewed;         541 AA.
AC   O00061;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Cytochrome P450 67;
DE            EC=1.14.-.-;
DE   AltName: Full=CYPLXVII;
DE   AltName: Full=Planta-induced rust protein 16;
DE   Flags: Fragment;
GN   Name=CYP67; Synonyms=PIG16;
OS   Uromyces fabae (Rust fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC   Pucciniomycetes; Pucciniales; Pucciniaceae; Uromyces.
OX   NCBI_TaxID=55588;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=I2; TISSUE=Haustorium;
RX   PubMed=9150592; DOI=10.1094/mpmi.1997.10.4.427;
RA   Hahn M., Mendgen K.;
RT   "Characterization of in planta-induced rust genes isolated from a
RT   haustorium-specific cDNA library.";
RL   Mol. Plant Microbe Interact. 10:427-437(1997).
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- DEVELOPMENTAL STAGE: Haustoria and rust-infected leaves. Also observed
CC       in non-germinated spores. After germination, expression stopped.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; U81793; AAB39881.1; -; mRNA.
DR   AlphaFoldDB; O00061; -.
DR   SMR; O00061; -.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   SUPFAM; SSF48264; SSF48264; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Metal-binding; Monooxygenase; Oxidoreductase.
FT   CHAIN           <1..541
FT                   /note="Cytochrome P450 67"
FT                   /id="PRO_0000052055"
FT   BINDING         479
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   541 AA;  61231 MW;  BC59B9C90CE3C4F5 CRC64;
     FIVLISATAH FIFRRREPTA FQYACFQAGL ALLLSVLLRE PFGAVVLILL ALNFLLGVQI
     ALYRLFWHDL RVFPGPRLAA ITQGWILREA YLGRSRFSMK EVGETYGDWV RIGPNELCTT
     SIEALSTIMG PKGWPKGPSY DSGITKGDSG GDSVLTIKNL PEHATRRRIW NKAFTPNAIN
     GYLPSIEIRL EEMLSVIDTE IKKGESVDLC LQLGCFVYDT MCDMAFGALA GSAFSKTQED
     KYRILTHMGR VVRQVGIVRN MPWLTPIVKA WPSSHRREQN EFKEFTKSMF LRRKNQGLGK
     QLDVFHYLLG EDTETGTRLT EAELAADSTL LVITGADTTR TVLLAFFLYL LKHPNYMEQL
     QAELLAAPDL SPPSLSRLEY LNACLQETMR LQPPSPANLQ RICPPGGAVI CGRQIPEGTK
     VRFSNYAIHR DERYFSRAEE FRPQRWLQKA KDDLGNQGEE KERLDQRAFF GFLIGPGACV
     AKNLAWMEMR LVVATILTNF DLSFAPGFDP VAFESSWTDA YLLLIEEPFE VMFTPKSQRM
     R