ID   CP6A1_MUSDO             Reviewed;         509 AA.
AC   P13527;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Cytochrome P450 6A1;
DE            EC=1.14.14.1;
DE   AltName: Full=CYPVIA1;
GN   Name=CYP6A1;
OS   Musca domestica (House fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Muscoidea;
OC   Muscidae; Musca.
OX   NCBI_TaxID=7370;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2922393; DOI=10.1073/pnas.86.5.1465;
RA   Feyereisen R., Koener J.F., Farnsworth D.E., Nebert D.W.;
RT   "Isolation and sequence of cDNA encoding a cytochrome P-450 from an
RT   insecticide-resistant strain of the house fly, Musca domestica.";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:1465-1469(1989).
CC   -!- FUNCTION: Involved in the metabolism of insect hormones and in the
CC       breakdown of synthetic insecticides.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an organic molecule + O2 + reduced [NADPH--hemoprotein
CC         reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein
CC         reductase]; Xref=Rhea:RHEA:17149, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC         COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:30879, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:142491; EC=1.14.14.1;
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral
CC       membrane protein. Microsome membrane; Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; L27242; AAA72423.1; -; Genomic_DNA.
DR   EMBL; M25367; AAA29293.1; -; mRNA.
DR   PIR; A32157; A32157.
DR   RefSeq; NP_001274159.1; NM_001287230.1.
DR   AlphaFoldDB; P13527; -.
DR   SMR; P13527; -.
DR   PRIDE; P13527; -.
DR   GeneID; 101889365; -.
DR   KEGG; mde:101889365; -.
DR   VEuPathDB; VectorBase:MDOA014776; -.
DR   eggNOG; KOG0158; Eukaryota.
DR   Proteomes; UP000095301; Whole Genome Shotgun Assembly.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070330; F:aromatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW   Monooxygenase; Oxidoreductase.
FT   CHAIN           1..509
FT                   /note="Cytochrome P450 6A1"
FT                   /id="PRO_0000051865"
FT   BINDING         449
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        70..73
FT                   /note="DPLP -> GPFA (in Ref. 1; AAA29293)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        372..373
FT                   /note="Missing (in Ref. 1; AAA29293)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   509 AA;  58731 MW;  229EF807F1DFF8F5 CRC64;
     MDFGSFLLYA LGVLASLALY FVRWNFGYWK RRGIPHEEPH LVMGNVKGLR SKYHIGEIIA
     DYYRKFKGSD PLPGIFLGHK PAAVVLDKEL RKRVLIKDFS NFANRGLYYN EKDDPLTGHL
     VMVEGEKWRS LRTKLSPTFT AGKMKYMYNT VLEVGQRLLE VMYEKLEVSS ELDMRDILAR
     FNTDVIGSVA FGIECNSLRN PHDRFLAMGR KSIEVPRHNA LIMAFIDSFP ELSRKLGMRV
     LPEDVHQFFM SSIKETVDYR EKNNIRRNDF LDLVLDLKNN PESISKLGGL TFNELAAQVF
     VFFLGGFETS SSTMGFALYE LAQNQQLQDR LREEVNEVFD QFKEDNISYD ALMNIPYLDQ
     VLNETLRKYP VGVGSALTRQ TLNDYVVPHN PKYVLPKGTL VFIPVLGIHY DPELYPNPEE
     FDPERFSPEM VKQRDSVDWL GFGDGPRNCI GMRFGKMQSR LGLALVIRHF RFTVCSRTDI
     PMQINPESLA WTPKNNLYLN VQAIRKKIK