CP6A8_DROME
ID CP6A8_DROME Reviewed; 506 AA.
AC Q27593; Q9V775;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Cytochrome P450 6a8;
DE EC=1.14.-.-;
DE AltName: Full=CYPVIA8;
GN Name=Cyp6a8; ORFNames=CG10248;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE, AND FUNCTION.
RC STRAIN=91-R;
RX PubMed=8973362; DOI=10.1016/s0378-1119(96)00446-5;
RA Maitra S., Dombrowski S.M., Waters L.C., Ganguly R.;
RT "Three second chromosome-linked clustered Cyp6 genes show differential
RT constitutive and barbital-induced expression in DDT-resistant and
RT susceptible strains of Drosophila melanogaster.";
RL Gene 180:165-171(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Involved in the metabolism of insect hormones and in the
CC breakdown of synthetic insecticides. {ECO:0000269|PubMed:8973362}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; L46859; AAB05550.1; -; mRNA.
DR EMBL; AE013599; AAF58185.2; -; Genomic_DNA.
DR PIR; JC5320; JC5320.
DR RefSeq; NP_523749.2; NM_079025.5.
DR AlphaFoldDB; Q27593; -.
DR SMR; Q27593; -.
DR BioGRID; 62408; 1.
DR STRING; 7227.FBpp0086585; -.
DR PaxDb; Q27593; -.
DR DNASU; 36666; -.
DR EnsemblMetazoa; FBtr0087455; FBpp0086585; FBgn0013772.
DR GeneID; 36666; -.
DR KEGG; dme:Dmel_CG10248; -.
DR CTD; 36666; -.
DR FlyBase; FBgn0013772; Cyp6a8.
DR VEuPathDB; VectorBase:FBgn0013772; -.
DR eggNOG; KOG0158; Eukaryota.
DR HOGENOM; CLU_001570_5_2_1; -.
DR InParanoid; Q27593; -.
DR OMA; MQIIWRL; -.
DR OrthoDB; 467733at2759; -.
DR PhylomeDB; Q27593; -.
DR BioGRID-ORCS; 36666; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 36666; -.
DR PRO; PR:Q27593; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0013772; Expressed in crop (Drosophila) and 19 other tissues.
DR ExpressionAtlas; Q27593; baseline and differential.
DR Genevisible; Q27593; DM.
DR GO; GO:0012505; C:endomembrane system; HDA:FlyBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0018685; F:alkane 1-monooxygenase activity; IDA:FlyBase.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0017143; P:insecticide metabolic process; IDA:FlyBase.
DR GO; GO:0048252; P:lauric acid metabolic process; IDA:FlyBase.
DR GO; GO:0031000; P:response to caffeine; IDA:FlyBase.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..506
FT /note="Cytochrome P450 6a8"
FT /id="PRO_0000051867"
FT BINDING 451
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT CONFLICT 4
FT /note="T -> A (in Ref. 1; AAB05550)"
FT /evidence="ECO:0000305"
FT CONFLICT 193
FT /note="G -> A (in Ref. 1; AAB05550)"
FT /evidence="ECO:0000305"
FT CONFLICT 332..343
FT /note="Missing (in Ref. 1; AAB05550)"
FT /evidence="ECO:0000305"
FT CONFLICT 465
FT /note="L -> P (in Ref. 1; AAB05550)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 506 AA; 58117 MW; A0B2A49DA3B99173 CRC64;
MALTYILFQV AVALLAILTY YIHRKLTYFK RRGIPFVAPH LIRGNMEELQ KTKNIHEIFQ
DHYNKFRESK APFVGFFFFQ SPAAFVIDLE LAKQILIKDF SNFSNKGIFY NEKDDPISAH
LFNLDGAQWR LLRNKLSSTF TSGKMKLMYP TVVSVANEFM TVMHEKVPKN SVLEIRDLVA
RFTVDVIGTC AFGIQCNSLR DEKAEFLYFG KRSLVDKRHG TLLNGFMRSY PKLARKLGMV
RTAPHIQEFY SRIVTETVAV REKEHIKRND FMDMLIELKN QKEMTLENGD VVRGLTMEEV
LAQAFVFFIA GFETSSSTMG FALYELAKNP DIQDKVRAEV EEVIEQHDQN FTYECTKDLK
YLNQVLDETL RLYTIVPNLD RMAAKRYVVP GHPNFVIEAG QSVIIPSSAI HHDPSIYPEP
FEFRPERFSP EESAGRPSVA WLPFGDGPRN CIGLRFGQMQ ARIGLALLIR NFKFSTCSKT
PNPLVYDPKS FVLGVKDGIY LKVETV
