CP6A9_DROME
ID CP6A9_DROME Reviewed; 504 AA.
AC Q27594; Q8MRY8; Q9V772;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 3.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Cytochrome P450 6a9;
DE EC=1.14.-.-;
DE AltName: Full=CYPVIA9;
GN Name=Cyp6a9; ORFNames=CG10246;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=91-R;
RX PubMed=8973362; DOI=10.1016/s0378-1119(96)00446-5;
RA Maitra S., Dombrowski S.M., Waters L.C., Ganguly R.;
RT "Three second chromosome-linked clustered Cyp6 genes show differential
RT constitutive and barbital-induced expression in DDT-resistant and
RT susceptible strains of Drosophila melanogaster.";
RL Gene 180:165-171(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Involved in the metabolism of insect hormones and in the
CC breakdown of synthetic insecticides. {ECO:0000269|PubMed:8973362}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB46609.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; L46860; AAB46609.1; ALT_INIT; mRNA.
DR EMBL; AE013599; AAF58188.2; -; Genomic_DNA.
DR EMBL; AY119196; AAM51056.1; -; mRNA.
DR PIR; JC5321; JC5321.
DR RefSeq; NP_523748.2; NM_079024.3.
DR AlphaFoldDB; Q27594; -.
DR SMR; Q27594; -.
DR BioGRID; 62405; 6.
DR IntAct; Q27594; 5.
DR STRING; 7227.FBpp0289405; -.
DR PaxDb; Q27594; -.
DR PRIDE; Q27594; -.
DR EnsemblMetazoa; FBtr0300128; FBpp0289405; FBgn0013771.
DR GeneID; 36663; -.
DR KEGG; dme:Dmel_CG10246; -.
DR CTD; 36663; -.
DR FlyBase; FBgn0013771; Cyp6a9.
DR VEuPathDB; VectorBase:FBgn0013771; -.
DR eggNOG; KOG0158; Eukaryota.
DR GeneTree; ENSGT00940000165972; -.
DR HOGENOM; CLU_001570_5_2_1; -.
DR InParanoid; Q27594; -.
DR OrthoDB; 467733at2759; -.
DR PhylomeDB; Q27594; -.
DR SignaLink; Q27594; -.
DR BioGRID-ORCS; 36663; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 36663; -.
DR PRO; PR:Q27594; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0013771; Expressed in adult Malpighian tubule (Drosophila) and 22 other tissues.
DR Genevisible; Q27594; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..504
FT /note="Cytochrome P450 6a9"
FT /id="PRO_0000051868"
FT BINDING 449
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT CONFLICT 137
FT /note="Y -> S (in Ref. 1; AAB46609 and 4; AAM51056)"
FT /evidence="ECO:0000305"
FT CONFLICT 376
FT /note="V -> D (in Ref. 1; AAB46609)"
FT /evidence="ECO:0000305"
FT CONFLICT 424..434
FT /note="DNFSPERVKER -> IFFARTSEGS (in Ref. 1; AAB46609)"
FT /evidence="ECO:0000305"
FT CONFLICT 448
FT /note="N -> L (in Ref. 1; AAB46609)"
FT /evidence="ECO:0000305"
FT CONFLICT 461
FT /note="S -> I (in Ref. 4; AAM51056)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 504 AA; 58005 MW; 3B0C875DAC3D4290 CRC64;
MGVYSVLLAI VVVLVGYLLL KWRRALHYWQ NLDIPCEEPH ILMGSLTGVQ TSRSFSAIWM
DYYNKFRGTG PFAGFYWFQR PGILVLDISL AKLILIKEFN KFTDRGFYHN TEDDPLSGQL
FLLDGQKWKS MRSKLSYTFT SGKMKYMFPT VVKVGHEFIE VFGQAMEKSP IVEVRDILAR
FTTDVIGTCA FGIECSSLKD PEAEFRVMGR RAIFEQRHGP IGIAFINSFQ NLARRLHMKI
TLEEAEHFFL RIVRETVAFR EKNNIRRNDF MDQLIDLKNS PLTKSESGES VNLTIEEMAA
QAFVFFGAGF ETSSTTMGFA LYELAQHQDI QDRVRKECQE VIGKYNGEIT YESMKDMVYL
DQVISETLRL YTVLPVLNRE CLEDYEVPGH PKYVIKKGMP VLIPCGAMHR DEKLYANPNT
FNPDNFSPER VKERDSVEWL PFGDGPRNCI GMRFGQMQAR SGLALLINRF KFSVCEQTTI
PIVYSKKTFL ISSETGIFLK VERV
