ID   CP6B1_PAPPO             Reviewed;         498 AA.
AC   Q04552; Q04553; Q27878;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Cytochrome P450 6B1;
DE            EC=1.14.14.1;
DE   AltName: Full=CYP6B1v1/CYP6B1v2/CYP6B1v3;
DE   AltName: Full=CYPVIB1;
GN   Name=CYP6B1;
OS   Papilio polyxenes (Black swallowtail butterfly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Papilionoidea;
OC   Papilionidae; Papilioninae; Papilio.
OX   NCBI_TaxID=7146;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 1-25 AND 401-406.
RC   TISSUE=Midgut;
RX   PubMed=1279697; DOI=10.1073/pnas.89.22.10920;
RA   Cohen M.B., Schuler M.A., Berenbaum M.R.;
RT   "A host-inducible cytochrome P-450 from a host-specific caterpillar:
RT   molecular cloning and evolution.";
RL   Proc. Natl. Acad. Sci. U.S.A. 89:10920-10924(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (CYP6B1V3).
RC   TISSUE=Midgut;
RX   PubMed=8065937; DOI=10.1093/nar/22.15.3210;
RA   Prapaipong H.H., Berenbaum M.M., Schuler M.M.;
RT   "Transcriptional regulation of the Papilio polyxenes CYP6B1 gene.";
RL   Nucleic Acids Res. 22:3210-3217(1994).
CC   -!- FUNCTION: Enables the insect to feed on furanocoumarin-producing plants
CC       and evolved as an adaptation for detoxification of xanthotoxin and
CC       other furanocoumarins.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an organic molecule + O2 + reduced [NADPH--hemoprotein
CC         reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein
CC         reductase]; Xref=Rhea:RHEA:17149, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC         COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:30879, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:142491; EC=1.14.14.1;
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral
CC       membrane protein. Microsome membrane; Peripheral membrane protein.
CC   -!- TISSUE SPECIFICITY: Midgut microsome.
CC   -!- INDUCTION: By xanthotoxin, a secondary metabolite abundant in the host
CC       plants of this specialized herbivore.
CC   -!- POLYMORPHISM: The sequence shown is that of 6B1-1, 6B1-2 seems to
CC       differ in 9 positions and is probably an allele.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; M80828; AAA29789.1; -; mRNA.
DR   EMBL; M83117; AAA29790.1; -; mRNA.
DR   EMBL; Z29624; CAA82732.1; -; Genomic_DNA.
DR   EMBL; U05037; AAA16154.1; -; Unassigned_DNA.
DR   PIR; S48058; S48058.
DR   AlphaFoldDB; Q04552; -.
DR   SMR; Q04552; -.
DR   BRENDA; 1.14.14.1; 7735.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070330; F:aromatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Endoplasmic reticulum; Heme; Iron; Membrane;
KW   Metal-binding; Microsome; Monooxygenase; Oxidoreductase.
FT   CHAIN           1..498
FT                   /note="Cytochrome P450 6B1"
FT                   /id="PRO_0000051893"
FT   BINDING         443
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
FT   VARIANT         24
FT                   /note="N -> D (in 6B1-2 and 6B1-3)"
FT   VARIANT         155..156
FT                   /note="NS -> KC (in 6B1-2 and 6B1-3)"
FT   VARIANT         243
FT                   /note="K -> R (in 6B1-2)"
FT   VARIANT         285
FT                   /note="A -> S (in 6B1-2)"
FT   VARIANT         293
FT                   /note="I -> V (in 6B1-2)"
FT   VARIANT         458
FT                   /note="M -> V (in 6B1-2)"
FT   VARIANT         475
FT                   /note="P -> E (in 6B1-2)"
FT   VARIANT         495
FT                   /note="L -> I (in 6B1-2)"
SQ   SEQUENCE   498 AA;  57484 MW;  043A849CA0990153 CRC64;
     MLYLLALVTV LAGLLHYYFT RTFNYWKKRN VAGPKPVPFF GNLKDSVLRR KPQVMVYKSI
     YDEFPNEKVV GIYRMTTPSV LLRDLDIIKH VLIKDFESFA DRGVEFSLDG LGANIFHADG
     DRWRSLRNRF TPLFTSGKLK SMLPLMSQVG DRFINSIDEV SQTQPEQSIH NLVQKFTMTN
     IAACVFGLNL DEGMLKTLED LDKHIFTVNY SAELDMMYPG ILKKLNGSLF PKVVSKFFDN
     LTKNVLEMRK GTPSYQKDMI DLIQELREKK TLELSRKHEN EDVKALELTD GVISAQMFIF
     YMAGYETSAT TMTYLFYELA KNPDIQDKLI AEIDEVLSRH DGNITYECLS EMTYLSKVFD
     ETLRKYPVAD FTQRNAKTDY VFPGTDITIK KGQTIIVSTW GIQNDPKYYP NPEKFDPERF
     NPENVKDRHP CAYLPFSAGP RNCLGMRFAK WQSEVCIMKV LSKYRVEPSM KSSGPFKFDP
     MRLFALPKGG IYVNLVRR