ID   CP6B4_PAPGL             Reviewed;         500 AA.
AC   Q27902; Q95035;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Cytochrome P450 6B4;
DE            EC=1.14.14.1;
DE   AltName: Full=CYP6B4v1/CYP6B4v2;
DE   AltName: Full=CYPVIB4;
GN   Name=CYP6B4;
OS   Papilio glaucus (Eastern tiger swallowtail butterfly) (Pterourus glaucus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Papilionoidea;
OC   Papilionidae; Papilioninae; Papilio; Pterourus.
OX   NCBI_TaxID=45779;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX   PubMed=9219364; DOI=10.1016/s0965-1748(97)00009-x;
RA   Hung C.F., Berenbaum M.R., Schuler M.A.;
RT   "Isolation and characterization of CYP6B4, a furanocoumarin-inducible
RT   cytochrome P450 from a polyphagous caterpillar (Lepidoptera:
RT   Papilionidae).";
RL   Insect Biochem. Mol. Biol. 27:377-385(1997).
CC   -!- FUNCTION: Enables the insect to feed on furanocoumarin-producing plants
CC       and evolved as an adaptation for detoxification of xanthotoxin and
CC       other furanocoumarins. This isozyme metabolizes isopimpinellin,
CC       imperatorin, and bergapten at high rates, xanthotoxin and psoralen at
CC       intermediate rates and angelicin, sphondin, and trioxsalen only at very
CC       low rates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an organic molecule + O2 + reduced [NADPH--hemoprotein
CC         reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein
CC         reductase]; Xref=Rhea:RHEA:17149, Rhea:RHEA-COMP:11964, Rhea:RHEA-
CC         COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:30879, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:142491; EC=1.14.14.1;
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral
CC       membrane protein. Microsome membrane; Peripheral membrane protein.
CC   -!- INDUCTION: By furnocoumarin.
CC   -!- POLYMORPHISM: The sequence shown is that of 6B4-1, 6B4-2 seems to
CC       differ in 7 positions and is probably an allele.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; U47059; AAB05892.1; -; mRNA.
DR   EMBL; U65489; AAB06742.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q27902; -.
DR   SMR; Q27902; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070330; F:aromatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW   Monooxygenase; Oxidoreductase.
FT   CHAIN           1..500
FT                   /note="Cytochrome P450 6B4"
FT                   /id="PRO_0000051896"
FT   BINDING         443
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
FT   VARIANT         17
FT                   /note="F -> L (in 6B4-2)"
FT   VARIANT         160
FT                   /note="F -> I (in 6B4-2)"
FT   VARIANT         229
FT                   /note="I -> L (in 6B4-2)"
FT   VARIANT         286
FT                   /note="L -> F (in 6B4-2)"
FT   VARIANT         477
FT                   /note="N -> S (in 6B4-2)"
FT   VARIANT         481
FT                   /note="K -> T (in 6B4-2)"
FT   VARIANT         496
FT                   /note="I -> L (in 6B4-2)"
SQ   SEQUENCE   500 AA;  58046 MW;  F6042D7E196029E2 CRC64;
     MLTIFIVTAT LFAILYFYFT RNFNYWKDRN VVGPEPTVFF GNIMESVIRR KHLIMIYKDI
     YEAFPKEKVV GIYRMTTPCL LLRDLDVIKH VMIKDFDLFN DRGVEFSEEG LGLNIFHADG
     DRWRVLRQCF TPLFTSGKLK NMLNLMSDRG DKFIKMVEKF CDKEPEQQII PLVRKFTMAS
     ITTCAFGMEL DEEMIETLDK LDSLIFTTSY GNEIDMMYPG ILKKLNSSIF SKMIAPFFDN
     LTKTIIEQRG GKPTNRKDLM DLILELRQKK AIEPMKKTHD EQVTTLELTD SVIAAQTFIF
     YAAGYETSAS TMSFLLFELA ENPDIQEKVI AEVDETLKRH NGEITYDTLS EMTYLTQVFH
     ETLRKYPVAD ILLRNAKADY AVPGTNVTLK KGQTVVVSGF GIHYDPKYYP DPEKFDPERF
     SPENVRNRHP CAYIPFGAGQ RKCLGMRFGQ WQVQVCIIKL LSKFRFEPST KTMSEFNYDP
     KRLLVYPKSG IFLNIIPRNY