CP6D1_MUSDO
ID CP6D1_MUSDO Reviewed; 516 AA.
AC Q27698; Q27697; Q27699; Q27700; Q9GU29; Q9XYB5;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Cytochrome P450 6d1;
DE EC=1.14.-.-;
DE AltName: Full=CYPVID1;
DE AltName: Full=Cytochrome P450 Ipr;
DE AltName: Full=Pyrethroid resistance cytochrome P450;
GN Name=CYP6D1;
OS Musca domestica (House fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Muscoidea;
OC Muscidae; Musca.
OX NCBI_TaxID=7370;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS LEU-2 DEL; LEU-55; ASP-150;
RP ILE-153; THR-165; GLU-218; ILE-220; 225-THR--MET-227; PRO-266 AND ILE-469.
RC STRAIN=aabys, CS, ISK, and LPR;
RX PubMed=8589839; DOI=10.1111/j.1365-2583.1995.tb00018.x;
RA Tomita T., Liu N., Smith F.F., Sridhar P., Scott J.G.;
RT "Molecular mechanisms involved in increased expression of a cytochrome P450
RT responsible for pyrethroid resistance in the housefly, Musca domestica.";
RL Insect Mol. Biol. 4:135-140(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS LEU-2 DEL; LEU-55; ASP-150;
RP ILE-153; THR-165; ASN-182; GLU-218; ILE-220; 225-THR--MET-227; THR-262;
RP PRO-266; ASN-447 AND ILE-469.
RC STRAIN=aabys, CS, ISK, LPR, and OCR;
RX PubMed=9931509; DOI=10.1016/s0378-1119(98)00545-9;
RA Scott J.G., Liu N., Wen Z., Smith F.F., Kasai S., Horak C.E.;
RT "House-fly cytochrome P450 CYP6D1: 5' flanking sequences and comparison of
RT alleles.";
RL Gene 226:347-353(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-31; 100-120; 284-289 AND
RP 411-437, AND VARIANTS ASP-150; ILE-153; THR-165; GLU-218; ILE-220;
RP 225-THR--MET-227 AND ILE-469.
RC STRAIN=LPR;
RX PubMed=7711755; DOI=10.1016/0965-1748(94)00066-q;
RA Tomita T., Scott J.G.;
RT "cDNA and deduced protein sequence of CYP6D1: the putative gene for a
RT cytochrome P450 responsible for pyrethroid resistance in house fly.";
RL Insect Biochem. Mol. Biol. 25:275-283(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS ASP-150; ILE-153; THR-165;
RP GLU-218; ILE-220; 225-THR--MET-227 AND ILE-469.
RC STRAIN=LPR;
RX PubMed=7875255; DOI=10.1007/bf01929363;
RA Liu N., Tomita T., Scott J.G.;
RT "Allele-specific PCR reveals that CYP6D1 is on chromosome 1 in the house
RT fly, Musca domestica.";
RL Experientia 51:164-167(1995).
RN [5]
RP NUCLEOTIDE SEQUENCE OF 1-229, AND VARIANT LEU-55.
RC STRAIN=Edinburgh;
RX PubMed=11437910; DOI=10.1046/j.1365-2583.2001.00256.x;
RA Kasai S., Scott J.G.;
RT "Cytochrome P450s CYP6D3 and CYP6D1 are part of a P450 gene cluster on
RT autosome 1 in house fly.";
RL Insect Mol. Biol. 10:191-196(2001).
CC -!- FUNCTION: Metabolizes pyrethroid insecticides and other xenobiotics.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Found only in adults and late pupae.
CC -!- INDUCTION: By phenobarbital in susceptible strains (3-fold in CS), but
CC not in the LPR strain.
CC -!- POLYMORPHISM: Alleles from the pyrethroid-susceptible strains CS
CC (CYP6D1v2) (shown here), aabys (CYP6D1v3), ISK (CYP6D1v4), OCR
CC (CYP6D1v5) and Edinburgh, and one allele from the pyrethroid-resistant
CC strain LPR (CYP6D1v1) are described here.
CC -!- MISCELLANEOUS: Expressed at higher levels in LPR compared to
CC susceptible flies, it is responsible for monooxygenase-mediated
CC pyrethroid resistance.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; U22367; AAC46931.1; -; mRNA.
DR EMBL; U22366; AAC46930.1; -; mRNA.
DR EMBL; AF064795; AAC99341.1; -; Genomic_DNA.
DR EMBL; AF081288; AAD32478.1; -; mRNA.
DR EMBL; U22362; AAC46932.1; -; mRNA.
DR EMBL; AF200191; AAG28564.1; -; Genomic_DNA.
DR EMBL; U15168; AAA81513.1; -; mRNA.
DR EMBL; AF064794; AAC99340.1; -; Genomic_DNA.
DR AlphaFoldDB; Q27698; -.
DR SMR; Q27698; -.
DR VEuPathDB; VectorBase:MDOA002847; -.
DR eggNOG; KOG0158; Eukaryota.
DR Proteomes; UP000095301; Whole Genome Shotgun Assembly.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Endoplasmic reticulum; Heme; Iron; Membrane;
KW Metal-binding; Microsome; Monooxygenase; Oxidoreductase.
FT CHAIN 1..516
FT /note="Cytochrome P450 6d1"
FT /id="PRO_0000051878"
FT BINDING 461
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT VARIANT 2
FT /note="Missing (in allele CYP6D1v3)"
FT /evidence="ECO:0000269|PubMed:8589839,
FT ECO:0000269|PubMed:9931509"
FT VARIANT 55
FT /note="M -> L (in allele CYP6D1v3 and allele CYP6D1v5)"
FT /evidence="ECO:0000269|PubMed:11437910,
FT ECO:0000269|PubMed:8589839, ECO:0000269|PubMed:9931509"
FT VARIANT 150
FT /note="A -> D (in allele CYP6D1v1)"
FT /evidence="ECO:0000269|PubMed:7711755,
FT ECO:0000269|PubMed:7875255, ECO:0000269|PubMed:8589839,
FT ECO:0000269|PubMed:9931509"
FT VARIANT 153
FT /note="L -> I (in allele CYP6D1v1)"
FT /evidence="ECO:0000269|PubMed:7711755,
FT ECO:0000269|PubMed:7875255, ECO:0000269|PubMed:8589839,
FT ECO:0000269|PubMed:9931509"
FT VARIANT 165
FT /note="S -> T (in allele CYP6D1v1)"
FT /evidence="ECO:0000269|PubMed:7711755,
FT ECO:0000269|PubMed:7875255, ECO:0000269|PubMed:8589839,
FT ECO:0000269|PubMed:9931509"
FT VARIANT 182
FT /note="I -> N (in allele CYP6D1v5)"
FT /evidence="ECO:0000269|PubMed:9931509"
FT VARIANT 218
FT /note="Q -> E (in allele CYP6D1v5 and allele CYP6D1v1)"
FT /evidence="ECO:0000269|PubMed:7711755,
FT ECO:0000269|PubMed:7875255, ECO:0000269|PubMed:8589839,
FT ECO:0000269|PubMed:9931509"
FT VARIANT 220
FT /note="F -> I (in allele CYP6D1v1)"
FT /evidence="ECO:0000269|PubMed:7711755,
FT ECO:0000269|PubMed:7875255, ECO:0000269|PubMed:8589839,
FT ECO:0000269|PubMed:9931509"
FT VARIANT 225..227
FT /note="NFI -> TFM (in allele CYP6D1v5 and allele CYP6D1v1)"
FT VARIANT 262
FT /note="K -> T (in allele CYP6D1v5)"
FT /evidence="ECO:0000269|PubMed:9931509"
FT VARIANT 266
FT /note="R -> P (in allele CYP6D1v3)"
FT /evidence="ECO:0000269|PubMed:8589839,
FT ECO:0000269|PubMed:9931509"
FT VARIANT 447
FT /note="D -> N (in allele CYP6D1v5)"
FT /evidence="ECO:0000269|PubMed:9931509"
FT VARIANT 469
FT /note="M -> I (in allele CYP6D1v4, allele CYP6D1v5 and
FT allele CYP6D1v1)"
FT /evidence="ECO:0000269|PubMed:7711755,
FT ECO:0000269|PubMed:7875255, ECO:0000269|PubMed:8589839,
FT ECO:0000269|PubMed:9931509"
SQ SEQUENCE 516 AA; 59173 MW; 654E32FB34847B16 CRC64;
MLLLLLLIVV TTLYIFAKLH YTKWERLGFE SDKATIPLGS MAKVFHKERP FGLVMSDIYD
KCHEKVVGIY LFFKPALLVR DAELARQILT TDFNSFHDRG LYVDEKNDPM SANLFVMEGQ
SWRTLRMKLA PSFSSGKLKG MFETVDDVAA KLLNHLNERL KDGQSHVLEI KSILTTYAVD
IIGSVIFGLE IDSFTHPDNE FRVLSDRLFN PKKSTMLQRF RNLSNFICPP LAKLLSRLGA
KDPITYRLRD IVKRTIEFRE EKGVVRKDLL QLFIQLRNTG KISDDNDKLW HDVESTAENL
KAMSIDMIAS NSFLFYIAGS ETTAATTSFT IYELAMYPEI LKKAQSEVDE CLQRHGLKPQ
GRLTYEAIQD MKYLDLCVME TTRKYPGLPF LNRKCTQDFQ VPDTKLTIPK ETGIIISLLG
IHRDPQYFPQ PEDYRPERFA DESKDYDPAA YMPFGEGPRH CIAQRMGVMN SKVALAKILA
NFNIQPMPRQ EVEFKFHSAP VLVPVNGLNV GLSKRW
