CP6G1_DROME
ID CP6G1_DROME Reviewed; 524 AA.
AC Q9V674; O76800; Q0GT65; Q3KN31; Q5MAT9; Q5MAU2; Q95SI8;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Cytochrome P450 6g1;
DE EC=1.14.-.-;
DE AltName: Full=CYPVIG1;
DE AltName: Full=Cyp6-like protein;
GN Name=Cyp6g1; Synonyms=CYP6-like, DDT-R, Rst(2)DDT; ORFNames=CG8453;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND POLYMORPHISM.
RC STRAIN=Canton-S, Hikone-R, and WC2;
RX PubMed=12351787; DOI=10.1126/science.1074170;
RA Daborn P.J., Yen J.L., Bogwitz M.R., Le Goff G., Feil E., Jeffers S.,
RA Tijet N., Perry T., Heckel D., Batterham P., Feyereisen R., Wilson T.G.,
RA ffrench-Constant R.H.;
RT "A single P450 allele associated with insecticide resistance in
RT Drosophila.";
RL Science 297:2253-2256(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT SER-316.
RC STRAIN=Riv02, Riv04, Riv05, Riv06, Riv11, Riv12, Riv14, Riv16, Riv17,
RC Riv18, Riv20, Riv23, Riv24, Riv26, Riv28, Riv29, Riv30, Riv32, Riv35,
RC Riv40, Riv46, Riv50, Riv51, Riv52, Riv59, Riv62, Riv63, and Riv65;
RX PubMed=16783023; DOI=10.1534/genetics.105.053611;
RA Panhuis T.M., Swanson W.J.;
RT "Molecular evolution and population genetic analysis of candidate female
RT reproductive genes in Drosophila.";
RL Genetics 173:2039-2047(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS VAL-123 AND SER-316.
RC STRAIN=91-C, 91-R, Canton-S, Hikone-R, and Wisconsin;
RX PubMed=17134855; DOI=10.1016/j.gene.2006.09.019;
RA Kuruganti S., Lam V., Zhou X., Bennett G., Pittendrigh B.R., Ganguly R.;
RT "High expression of Cyp6g1, a cytochrome P450 gene, does not necessarily
RT confer DDT resistance in Drosophila melanogaster.";
RL Gene 388:43-53(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Larva;
RA Danielson P.B., Al-Zahrani A., Reeves S.A., Fogleman J.C.;
RT "Isolation of a novel CYP6-like cytochrome P450 from Drosophila
RT melanogaster.";
RL Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Buzeli R.A., Pedra J.H.F., Scharf M., Pittendrigh B.R.;
RT "Drosophila melanogaster CYP6G1 complete cDNA.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [7]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 216-524.
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [10]
RP FUNCTION.
RC STRAIN=Canton-S, Hikone-R, and Wisconsin-1;
RX PubMed=11810226; DOI=10.1007/s004380100531;
RA Daborn P., Boundy S., Yen J., Pittendrigh B., ffrench-Constant R.H.;
RT "DDT resistance in Drosophila correlates with Cyp6g1 over-expression and
RT confers cross-resistance to the neonicotinoid imidacloprid.";
RL Mol. Genet. Genomics 266:556-563(2001).
RN [11]
RP FUNCTION, DEVELOPMENTAL STAGE, AND POLYMORPHISM.
RX PubMed=15663776; DOI=10.1111/j.1365-2583.2005.00532.x;
RA Festucci-Buselli R.A., Carvalho-Dias A.S., de Oliveira-Andrade M.,
RA Caixeta-Nunes C., Li H.-M., Stuart J.J., Muir W., Scharf M.E.,
RA Pittendrigh B.R.;
RT "Expression of Cyp6g1 and Cyp12d1 in DDT resistant and susceptible strains
RT of Drosophila melanogaster.";
RL Insect Mol. Biol. 14:69-77(2005).
CC -!- FUNCTION: Necessary and sufficient for resistance to insecticides DDT
CC and imidacloprid. May be involved in the metabolism of insect hormones.
CC {ECO:0000269|PubMed:11810226, ECO:0000269|PubMed:12351787,
CC ECO:0000269|PubMed:15663776}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development, low levels in
CC embryos and first and second instar larvae, levels increase from third
CC instar through to adult. {ECO:0000269|PubMed:15663776}.
CC -!- POLYMORPHISM: 91-R, Hikone-R, WC2 and Wisconsin are insecticide
CC resistant strains, 91-C, Canton-S and Oregon-RC are insecticide
CC susceptible. Cyp6g1 is more highly expressed in the resistant strains.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL28318.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; DQ539119; ABG02640.1; -; Genomic_DNA.
DR EMBL; DQ539120; ABG02641.1; -; Genomic_DNA.
DR EMBL; DQ539121; ABG02642.1; -; Genomic_DNA.
DR EMBL; DQ539122; ABG02643.1; -; Genomic_DNA.
DR EMBL; DQ539123; ABG02644.1; -; Genomic_DNA.
DR EMBL; DQ539124; ABG02645.1; -; Genomic_DNA.
DR EMBL; DQ539125; ABG02646.1; -; Genomic_DNA.
DR EMBL; DQ539126; ABG02647.1; -; Genomic_DNA.
DR EMBL; DQ539127; ABG02648.1; -; Genomic_DNA.
DR EMBL; DQ539128; ABG02649.1; -; Genomic_DNA.
DR EMBL; DQ539129; ABG02650.1; -; Genomic_DNA.
DR EMBL; DQ539130; ABG02651.1; -; Genomic_DNA.
DR EMBL; DQ539131; ABG02652.1; -; Genomic_DNA.
DR EMBL; DQ539132; ABG02653.1; -; Genomic_DNA.
DR EMBL; DQ539133; ABG02654.1; -; Genomic_DNA.
DR EMBL; DQ539134; ABG02655.1; -; Genomic_DNA.
DR EMBL; DQ539135; ABG02656.1; -; Genomic_DNA.
DR EMBL; DQ539136; ABG02657.1; -; Genomic_DNA.
DR EMBL; DQ539137; ABG02658.1; -; Genomic_DNA.
DR EMBL; DQ539138; ABG02659.1; -; Genomic_DNA.
DR EMBL; DQ539139; ABG02660.1; -; Genomic_DNA.
DR EMBL; DQ539140; ABG02661.1; -; Genomic_DNA.
DR EMBL; DQ539141; ABG02662.1; -; Genomic_DNA.
DR EMBL; DQ539142; ABG02663.1; -; Genomic_DNA.
DR EMBL; DQ539143; ABG02664.1; -; Genomic_DNA.
DR EMBL; DQ539144; ABG02665.1; -; Genomic_DNA.
DR EMBL; DQ539145; ABG02666.1; -; Genomic_DNA.
DR EMBL; DQ539146; ABG02667.1; -; Genomic_DNA.
DR EMBL; AY842137; AAW28866.1; -; Genomic_DNA.
DR EMBL; AY842138; AAW28867.1; -; Genomic_DNA.
DR EMBL; AY842139; AAW28868.1; -; Genomic_DNA.
DR EMBL; AY842140; AAW28869.1; -; Genomic_DNA.
DR EMBL; AY842141; AAW28870.1; -; Genomic_DNA.
DR EMBL; AF083946; AAC33298.1; -; mRNA.
DR EMBL; AY081960; AAL89788.1; -; mRNA.
DR EMBL; AE013599; AAF58557.1; -; Genomic_DNA.
DR EMBL; BT023908; ABA81842.1; -; mRNA.
DR EMBL; AY060770; AAL28318.1; ALT_INIT; mRNA.
DR RefSeq; NP_001286335.1; NM_001299406.1.
DR RefSeq; NP_610743.2; NM_136899.4.
DR AlphaFoldDB; Q9V674; -.
DR SMR; Q9V674; -.
DR IntAct; Q9V674; 2.
DR STRING; 7227.FBpp0087100; -.
DR PaxDb; Q9V674; -.
DR PRIDE; Q9V674; -.
DR DNASU; 36316; -.
DR EnsemblMetazoa; FBtr0087992; FBpp0087100; FBgn0025454.
DR EnsemblMetazoa; FBtr0339596; FBpp0308668; FBgn0025454.
DR GeneID; 36316; -.
DR KEGG; dme:Dmel_CG8453; -.
DR CTD; 36316; -.
DR FlyBase; FBgn0025454; Cyp6g1.
DR VEuPathDB; VectorBase:FBgn0025454; -.
DR eggNOG; KOG0158; Eukaryota.
DR GeneTree; ENSGT00940000167661; -.
DR HOGENOM; CLU_001570_5_2_1; -.
DR InParanoid; Q9V674; -.
DR OMA; GRCDPHG; -.
DR OrthoDB; 786853at2759; -.
DR PhylomeDB; Q9V674; -.
DR BioGRID-ORCS; 36316; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36316; -.
DR PRO; PR:Q9V674; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0025454; Expressed in adult Malpighian tubule (Drosophila) and 25 other tissues.
DR ExpressionAtlas; Q9V674; baseline and differential.
DR Genevisible; Q9V674; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0046701; P:insecticide catabolic process; IDA:FlyBase.
DR GO; GO:0046680; P:response to DDT; IMP:FlyBase.
DR GO; GO:0017085; P:response to insecticide; IMP:UniProtKB.
DR GO; GO:0046689; P:response to mercury ion; IMP:FlyBase.
DR GO; GO:0046683; P:response to organophosphorus; IMP:FlyBase.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..524
FT /note="Cytochrome P450 6g1"
FT /id="PRO_0000051883"
FT BINDING 458
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT VARIANT 123
FT /note="F -> V (in strain: Wisconsin)"
FT /evidence="ECO:0000269|PubMed:17134855"
FT VARIANT 316
FT /note="T -> S (in strain: Riv04, Riv14, Riv16, Riv17,
FT Riv18, Riv35, Riv59 and Wisconsin)"
FT /evidence="ECO:0000269|PubMed:16783023,
FT ECO:0000269|PubMed:17134855"
FT CONFLICT 31..34
FT /note="KGIP -> RHT (in Ref. 4; AAC33298)"
FT /evidence="ECO:0000305"
FT CONFLICT 39..43
FT /note="TPIIG -> RDHCNTKVVFKWTLGDLC (in Ref. 4; AAC33298)"
FT /evidence="ECO:0000305"
FT CONFLICT 59
FT /note="L -> P (in Ref. 4; AAC33298)"
FT /evidence="ECO:0000305"
FT CONFLICT 108..120
FT /note="ARCDPHGDPLGYN -> GRLRIPWRSIGLY (in Ref. 4;
FT AAC33298)"
FT /evidence="ECO:0000305"
FT CONFLICT 129..133
FT /note="HWKGI -> IGREF (in Ref. 4; AAC33298)"
FT /evidence="ECO:0000305"
FT CONFLICT 153
FT /note="M -> L (in Ref. 4; AAC33298)"
FT /evidence="ECO:0000305"
FT CONFLICT 274
FT /note="D -> S (in Ref. 4; AAC33298)"
FT /evidence="ECO:0000305"
FT CONFLICT 304..305
FT /note="AQ -> VS (in Ref. 4; AAC33298)"
FT /evidence="ECO:0000305"
FT CONFLICT 474
FT /note="S -> I (in Ref. 8; ABA81842)"
FT /evidence="ECO:0000305"
FT CONFLICT 500
FT /note="L -> H (in Ref. 4; AAC33298)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 524 AA; 59867 MW; 35F41CCCA866756F CRC64;
MVLTEVLFVV VAALVALYTW FQRNHSYWQR KGIPYIPPTP IIGNTKVVFK MENSFGMHLS
EIYNDPRLKD EAVVGIYSMN KPGLIIRDIE LIKSILIKDF NRFHNRYARC DPHGDPLGYN
NLFFVRDAHW KGIRTKLTPV FTSGKVKQMY TLMQEIGKDL ELALQRRGEK NSGSFITEIK
EICAQFSTDS IATIAFGIRA NSLENPNAEF RNYGRKMFTF TVARAKDFFV AFFLPKLVSL
MRIQFFTADF SHFMRSTIGH VMEERERSGL LRNDLIDVLV SLRKEAAAEP SKPHYAKNQD
FLVAQAGVFF TAGFETSSST MSFALYEMAK HPEMQKRLRD EINEALVEGG GSLSYEKIQS
LEYLAMVVDE VLRMYPVLPF LDREYESVEG QPDLSLKPFY DYTLENGTPV FIPIYALHHD
PKYWTNPSQF DPERFSPANR KNIVAMAYQP FGSGPHNCIG SRIGLLQSKL GLVSLLKNHS
VRNCEATMKD MKFDPKGFVL QADGGIHLEI VNDRLYDQSA PSLQ
