CP6G2_DROME
ID CP6G2_DROME Reviewed; 519 AA.
AC Q9V675; A8WHF1; Q6SEI3;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Probable cytochrome P450 6g2;
DE EC=1.14.-.-;
DE AltName: Full=CYPVIG2;
GN Name=Cyp6g2; ORFNames=CG8859;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=15663776; DOI=10.1111/j.1365-2583.2005.00532.x;
RA Festucci-Buselli R.A., Carvalho-Dias A.S., de Oliveira-Andrade M.,
RA Caixeta-Nunes C., Li H.-M., Stuart J.J., Muir W., Scharf M.E.,
RA Pittendrigh B.R.;
RT "Expression of Cyp6g1 and Cyp12d1 in DDT resistant and susceptible strains
RT of Drosophila melanogaster.";
RL Insect Mol. Biol. 14:69-77(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May be involved in the metabolism of insect hormones and in
CC the breakdown of synthetic insecticides. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: More highly expressed in DDT-susceptible strains
CC (Canton-S and 91-C) as compared to the DDT-resistant strains (91-R and
CC Wisconsin).
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AY459353; AAR88134.1; -; mRNA.
DR EMBL; AE013599; AAF58556.1; -; Genomic_DNA.
DR EMBL; BT031097; ABX00719.1; -; mRNA.
DR RefSeq; NP_610744.1; NM_136900.2.
DR AlphaFoldDB; Q9V675; -.
DR SMR; Q9V675; -.
DR BioGRID; 62095; 1.
DR DIP; DIP-19705N; -.
DR IntAct; Q9V675; 1.
DR STRING; 7227.FBpp0087117; -.
DR PaxDb; Q9V675; -.
DR PRIDE; Q9V675; -.
DR DNASU; 36317; -.
DR EnsemblMetazoa; FBtr0088009; FBpp0087117; FBgn0033696.
DR GeneID; 36317; -.
DR KEGG; dme:Dmel_CG8859; -.
DR UCSC; CG8859-RA; d. melanogaster.
DR CTD; 36317; -.
DR FlyBase; FBgn0033696; Cyp6g2.
DR VEuPathDB; VectorBase:FBgn0033696; -.
DR eggNOG; KOG0158; Eukaryota.
DR GeneTree; ENSGT00940000167661; -.
DR HOGENOM; CLU_001570_5_2_1; -.
DR InParanoid; Q9V675; -.
DR OMA; SLEYMQM; -.
DR OrthoDB; 786853at2759; -.
DR PhylomeDB; Q9V675; -.
DR BioGRID-ORCS; 36317; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36317; -.
DR PRO; PR:Q9V675; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033696; Expressed in embryonic/larval corpus allatum (Drosophila) and 5 other tissues.
DR Genevisible; Q9V675; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0046701; P:insecticide catabolic process; IBA:GO_Central.
DR GO; GO:0046680; P:response to DDT; IBA:GO_Central.
DR GO; GO:0017085; P:response to insecticide; IMP:FlyBase.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00465; EP450IV.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..519
FT /note="Probable cytochrome P450 6g2"
FT /id="PRO_0000051884"
FT BINDING 460
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT CONFLICT 442
FT /note="R -> H (in Ref. 1; AAR88134)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 519 AA; 59608 MW; 8C1770B4264CF8FB CRC64;
MELVLLILVA SLIGIAFLAL QQHYSYWRRM GVREIRPKWI VGNLMGLLNM RMSPAEFISQ
LYNHPDAENE PFVGIHVFHK PALLLRDPEM VRNILVKDFA GFSNRYSSSD PKGDPLGSQN
IFFLKNPAWK EVRLKLSPFF TGNRLKQMFP LIEEVGASLD AHLRQQPLHN ERMRCFDLEA
KELCALYTTD VIATVAYGVS ANSFTDPKCE FRRHGRSVFE FNLLRAAEFT LVFFLPHLVP
FVRFKVVPAE ATRFLRKTIN YVMSEREKSG QKRNDLIDIL IEFRRSTQLA KASGIKDQFV
FEGDILVAQA VLFFTAGFES SSSTMAFAMY ELAKDTDVQQ RLREEIKDAL VESGGQVTLK
MIESLEFMQM ILLEVLRMYP PLPFLDRECT SGRDYSLAPF HKKFVVPKGM PVYIPCYALH
MDPQYFPQPR KFLPERFSPE NRKLHTPYTY MPFGLGPHGC IGERFGYLQA KVGLVNLLRN
HMITTSERTP HRMQLDPKAI ITQAKGGIHL RLVRDALGV
