CP6T3_DROME
ID CP6T3_DROME Reviewed; 501 AA.
AC Q9V676;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Probable cytochrome P450 6t3;
DE EC=1.14.-.-;
DE AltName: Full=CYPVIT3;
GN Name=Cyp6t3; ORFNames=CG8457;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: May be involved in the metabolism of insect hormones and in
CC the breakdown of synthetic insecticides. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF58555.1; -; Genomic_DNA.
DR RefSeq; NP_610745.1; NM_136901.2.
DR AlphaFoldDB; Q9V676; -.
DR SMR; Q9V676; -.
DR BioGRID; 62096; 9.
DR DIP; DIP-20703N; -.
DR IntAct; Q9V676; 4.
DR STRING; 7227.FBpp0087101; -.
DR PaxDb; Q9V676; -.
DR EnsemblMetazoa; FBtr0087993; FBpp0087101; FBgn0033697.
DR GeneID; 36318; -.
DR KEGG; dme:Dmel_CG8457; -.
DR UCSC; CG8457-RA; d. melanogaster.
DR CTD; 36318; -.
DR FlyBase; FBgn0033697; Cyp6t3.
DR VEuPathDB; VectorBase:FBgn0033697; -.
DR eggNOG; KOG0158; Eukaryota.
DR GeneTree; ENSGT00940000167661; -.
DR HOGENOM; CLU_001570_5_2_1; -.
DR InParanoid; Q9V676; -.
DR OMA; AKYHNWR; -.
DR OrthoDB; 1247045at2759; -.
DR PhylomeDB; Q9V676; -.
DR SignaLink; Q9V676; -.
DR BioGRID-ORCS; 36318; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36318; -.
DR PRO; PR:Q9V676; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033697; Expressed in antenna and 6 other tissues.
DR ExpressionAtlas; Q9V676; baseline and differential.
DR Genevisible; Q9V676; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0006697; P:ecdysone biosynthetic process; IMP:FlyBase.
DR GO; GO:0046701; P:insecticide catabolic process; IBA:GO_Central.
DR GO; GO:0048638; P:regulation of developmental growth; IMP:FlyBase.
DR GO; GO:0046680; P:response to DDT; IBA:GO_Central.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..501
FT /note="Probable cytochrome P450 6t3"
FT /id="PRO_0000051889"
FT BINDING 444
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
SQ SEQUENCE 501 AA; 57845 MW; 84692FA41419C333 CRC64;
MLLIWLLLLT IVTLNFWLRH KYDYFRSRGI PHLPPSSWSP MGNLGQLLFL RISFGDLFRQ
LYADPRNGQA KIVGFFIFQT PALMVRDPEL IRQVLIKNFN NFLNRFESAD AGDPMGALTL
PLAKYHHWKE SRQCMSQLFT SGRMRDVMYS QMLDVASDLE QYLNRKLGDR LERVLPLGRM
CQLYTTDVTG NLFYSLNVGG LRRGRSELIT KTKELFNTNP RKVLDFMSVF FLPKWTGVLK
PKVFTEDYAR YMRHLVDDHH EPTKGDLINQ LQHFQLSRSS NHYSQHPDFV ASQAGIILLA
GFETSSALMG FTLYELAKAP DIQERLRSEL REAFISTATL SYDTLMTLPY LKMVCLEALR
LYPAAAFVNR ECTSSASEGF SLQPHVDFIV PPGMPAYISI LGLHRDERFW PEPCVFDPER
FGPERSRHIH PMTYIPFGAG PHGCIGSRLG VLQLKLGIVH ILKQYWVETC ERTVSEIRFN
PKSFMLESEN EIYLRFCRSS L
