CP9F2_DROME
ID CP9F2_DROME Reviewed; 516 AA.
AC Q9VG82;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Probable cytochrome P450 9f2;
DE EC=1.14.-.-;
DE AltName: Full=CYPIXF2;
GN Name=Cyp9f2; ORFNames=CG11466;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: May be involved in the metabolism of insect hormones and in
CC the breakdown of synthetic insecticides. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=Q9VG82-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q9VG82-2; Sequence=VSP_010295;
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AE014297; AAF54803.1; -; Genomic_DNA.
DR EMBL; AE014297; AAS65143.1; -; Genomic_DNA.
DR EMBL; AY058466; AAL13695.1; -; mRNA.
DR RefSeq; NP_650189.1; NM_141932.3. [Q9VG82-1]
DR AlphaFoldDB; Q9VG82; -.
DR SMR; Q9VG82; -.
DR BioGRID; 66620; 31.
DR DIP; DIP-18506N; -.
DR STRING; 7227.FBpp0082070; -.
DR PaxDb; Q9VG82; -.
DR PRIDE; Q9VG82; -.
DR DNASU; 41520; -.
DR EnsemblMetazoa; FBtr0082598; FBpp0082070; FBgn0038037. [Q9VG82-1]
DR GeneID; 41520; -.
DR KEGG; dme:Dmel_CG11466; -.
DR UCSC; CG11466-RA; d. melanogaster. [Q9VG82-1]
DR CTD; 41520; -.
DR FlyBase; FBgn0038037; Cyp9f2.
DR VEuPathDB; VectorBase:FBgn0038037; -.
DR eggNOG; KOG0158; Eukaryota.
DR GeneTree; ENSGT00940000165057; -.
DR HOGENOM; CLU_001570_5_2_1; -.
DR InParanoid; Q9VG82; -.
DR OMA; WIKLVQR; -.
DR PhylomeDB; Q9VG82; -.
DR SignaLink; Q9VG82; -.
DR BioGRID-ORCS; 41520; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 41520; -.
DR PRO; PR:Q9VG82; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0038037; Expressed in oviduct (Drosophila) and 28 other tissues.
DR ExpressionAtlas; Q9VG82; baseline and differential.
DR Genevisible; Q9VG82; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0035220; P:wing disc development; IMP:FlyBase.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Endoplasmic reticulum; Heme; Iron; Membrane;
KW Metal-binding; Microsome; Monooxygenase; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..516
FT /note="Probable cytochrome P450 9f2"
FT /id="PRO_0000051920"
FT BINDING 460
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..81
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000305"
FT /id="VSP_010295"
SQ SEQUENCE 516 AA; 60279 MW; 3666745A8BDC9D15 CRC64;
MLWEFFALFA IAAALFYRWA SANNDFFKDR GIAYEKPVLY FGNMAGMFLR KRAMFDIVCD
LYTKGGSKKF FGIFEQRQPL LMVRDPDLIK QITIKDFDHF INHRNVFATS SDDDPHDMSN
LFGSSLFSMR DARWKDMRST LSPAFTGSKM RQMFQLMNQV AKEAVDCLKQ DDSRVQENEL
DMKDYCTRFT NDVIASTAFG LQVNSFKDRE NTFYQMGKKL TTFTFLQSMK FMLFFALKGL
NKILKVELFD RKSTQYFVRL VLDAMKYRQE HNIVRPDMIN MLMEARGIIQ TEKTKASAVR
EWSDRDIVAQ CFVFFFAGFE TSAVLMCFTA HELMENQDVQ QRLYEEVQQV DQDLEGKELT
YEAIMGMKYL DQVVNEVLRK WPAAIAVDRE CNKDITFDVD GQKVEVKKGD VIWLPTCGFH
RDPKYFENPM KFDPERFSDE NKESIQPFTY FPFGLGQRNC IGSRFALLEA KAVIYYLLKD
YRFAPAKKSC IPLELITSGF QLSPKGGFWI KLVQRN
