ID   CPAO_ASPOR              Reviewed;         455 AA.
AC   Q2UG11;
DT   29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Beta-cyclopiazonate dehydrogenase {ECO:0000303|PubMed:21608094};
DE            EC=1.21.99.1 {ECO:0000303|PubMed:21608094};
DE   AltName: Full=Beta-Cyclopiazonate oxidocyclase {ECO:0000303|PubMed:21608094};
DE   AltName: Full=FAD-dependent oxidoreductase cpaO {ECO:0000305};
DE   Flags: Precursor;
GN   Name=cpaO {ECO:0000250|UniProtKB:F5HN72};
GN   ORFNames=AO090026000003 {ECO:0000312|EMBL:BAE59504.1};
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=510516 {ECO:0000312|EMBL:BAE59504.1};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA   Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA   Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA   Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA   Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA   Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA   Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA   Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA   Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA   Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA   Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA   Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=NBRC 4177;
RX   PubMed=21608094; DOI=10.1002/cbic.201000672;
RA   Kato N., Tokuoka M., Shinohara Y., Kawatani M., Uramoto M., Seshime Y.,
RA   Fujii I., Kitamoto K., Takahashi T., Takahashi S., Koyama Y., Osada H.;
RT   "Genetic safeguard against mycotoxin cyclopiazonic acid production in
RT   Aspergillus oryzae.";
RL   ChemBioChem 12:1376-1382(2011).
CC   -!- FUNCTION: Beta-cyclopiazonate dehydrogenase involved in the synthesis
CC       of the fungal neurotoxin alpha-cyclopiazonic acid (CPA). CpaO carries
CC       out the dehydrogenation of beta-CPA to yield an unstable enimine
CC       product, which is captured by intramolecular cyclization to create the
CC       pentacyclic fused scaffold of alpha-cyclopiazonate.
CC       {ECO:0000303|PubMed:21608094}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=A + beta-cyclopiazonate = AH2 + alpha-cyclopiazonate;
CC         Xref=Rhea:RHEA:14525, ChEBI:CHEBI:13193, ChEBI:CHEBI:17499,
CC         ChEBI:CHEBI:58067, ChEBI:CHEBI:58256; EC=1.21.99.1;
CC         Evidence={ECO:0000303|PubMed:21608094};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000250|UniProtKB:B8NI10};
CC   -!- SIMILARITY: Belongs to the beta-cyclopiazonate dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; AP007159; BAE59504.1; -; Genomic_DNA.
DR   RefSeq; XP_001821506.1; XM_001821454.2.
DR   AlphaFoldDB; Q2UG11; -.
DR   SMR; Q2UG11; -.
DR   EnsemblFungi; BAE59504; BAE59504; AO090026000003.
DR   GeneID; 5993534; -.
DR   KEGG; aor:AO090026000003; -.
DR   VEuPathDB; FungiDB:AO090026000003; -.
DR   HOGENOM; CLU_028280_0_0_1; -.
DR   OMA; WASDYST; -.
DR   Proteomes; UP000006564; Chromosome 3.
DR   GO; GO:0050448; F:beta-cyclopiazonate dehydrogenase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   1: Evidence at protein level;
KW   FAD; Flavoprotein; Oxidoreductase; Reference proteome; Signal; Virulence.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..455
FT                   /note="Beta-cyclopiazonate dehydrogenase"
FT                   /id="PRO_0000430692"
SQ   SEQUENCE   455 AA;  50983 MW;  387A45ECE71D7A72 CRC64;
     MAVRIARFLG LSTVAYLALA NGIDARDTIS RDVIILGGGS SGTYAAIRLR DQGKTVAVVE
     RNNYLGGHGE TYYTEDNTPL NFGVEGFFNT TVTRNYLERL QVPYGRRDPA PAHEDYVNLN
     TGQRTEYTPG QLQDREAFAK WVDAISQFGF LDDGVYRIPE PVPEDLISPF ADFVKKYHLE
     DAVYALFSHT SGDVLEMITL YVIQYIGVPH AAALNEGYVR PIEGIAALYK SAGKELGSDV
     LLETTPEAVQ RFEDGVEVIV RSADGTKTLL KGKQLLVTIP PLLENLHGFP LSDQESRLFS
     KWQYHQYWAA LVNDTGLPDD VNIVNVDTER LYGVPEEPFI WRLDNHWAPG YHNIKLVGGS
     EFGEDEAKAY MYERLDLLHA EGTYATHKPE IVKFASHTPV TMFVSAEEIR GGFYRQLYEL
     QGLNSTFWTG ATWASDYSTL LWGYTDEVLD QMASS