ID   CPAS1_DICDI             Reviewed;         842 AA.
AC   Q75J93; Q54ZR3;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Circularly permutated Ras protein 1;
DE            Short=DdiCPRas1;
GN   Name=cpras1; ORFNames=DDB_G0277381;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=18510733; DOI=10.1186/1745-6150-3-21;
RA   Elias M., Novotny M.;
RT   "cpRAS: a novel circularly permuted RAS-like GTPase domain with a highly
RT   scattered phylogenetic distribution.";
RL   Biol. Direct 3:21-21(2008).
CC   -!- DOMAIN: In contrast to other GTP-binding proteins, it is characterized
CC       by a circular permutation of the GTPase motifs described by a G4-G3-G1
CC       pattern.
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. CpRas family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000019; EAL68747.1; -; Genomic_DNA.
DR   RefSeq; XP_642673.1; XM_637581.1.
DR   AlphaFoldDB; Q75J93; -.
DR   SMR; Q75J93; -.
DR   STRING; 44689.DDB0229980; -.
DR   PaxDb; Q75J93; -.
DR   EnsemblProtists; EAL68747; EAL68747; DDB_G0277381.
DR   GeneID; 8621003; -.
DR   KEGG; ddi:DDB_G0277381; -.
DR   dictyBase; DDB_G0277381; cpras1.
DR   eggNOG; KOG0395; Eukaryota.
DR   HOGENOM; CLU_329360_0_0_1; -.
DR   InParanoid; Q75J93; -.
DR   OMA; CTDGMPN; -.
DR   PhylomeDB; Q75J93; -.
DR   Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR   Reactome; R-DDI-171007; p38MAPK events.
DR   Reactome; R-DDI-5218921; VEGFR2 mediated cell proliferation.
DR   Reactome; R-DDI-5621575; CD209 (DC-SIGN) signaling.
DR   Reactome; R-DDI-5658442; Regulation of RAS by GAPs.
DR   Reactome; R-DDI-5673000; RAF activation.
DR   Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR   Reactome; R-DDI-5674135; MAP2K and MAPK activation.
DR   Reactome; R-DDI-5675221; Negative regulation of MAPK pathway.
DR   Reactome; R-DDI-6798695; Neutrophil degranulation.
DR   Reactome; R-DDI-8849471; PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.
DR   Reactome; R-DDI-9648002; RAS processing.
DR   PRO; PR:Q75J93; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0030127; C:COPII vesicle coat; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0019003; F:GDP binding; IBA:GO_Central.
DR   GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR   GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IEA:InterPro.
DR   GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR   GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 2.
DR   Gene3D; 3.40.50.410; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR001806; Small_GTPase.
DR   InterPro; IPR020849; Small_GTPase_Ras-type.
DR   InterPro; IPR002035; VWF_A.
DR   InterPro; IPR036465; vWFA_dom_sf.
DR   InterPro; IPR036174; Znf_Sec23_Sec24_sf.
DR   PANTHER; PTHR24070; PTHR24070; 1.
DR   Pfam; PF00071; Ras; 2.
DR   SMART; SM00174; RHO; 1.
DR   SMART; SM00327; VWA; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   SUPFAM; SSF53300; SSF53300; 1.
DR   SUPFAM; SSF82919; SSF82919; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS51421; RAS; 1.
DR   PROSITE; PS50234; VWFA; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..842
FT                   /note="Circularly permutated Ras protein 1"
FT                   /id="PRO_0000371347"
FT   DOMAIN          377..627
FT                   /note="VWFA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT   REGION          253..274
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        255..274
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         62..66
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         121..124
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         181..188
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   842 AA;  93475 MW;  7A5C1A67E4887F22 CRC64;
     MEFPTKYVYI TSNLDKPKEW FTQSEMSLTT TTDTIQKSFV NNSGSSSSSK GFGEAVLLDI
     LDTAGQEEYS AMRDQYVRTG DCFMIVFSID SRSSFEEVSQ LKQHIERVKD RDDVPIIIVG
     NKVDLESRRQ VSRIEADQLA RSLRVPYIET SAKTRSNIEE AFFTLVRHTP RNTVYKVVVM
     GGGGVGKSAI IIQFIQNHFV EEYDPTIEDS YRKQVTISGL PPIGGSLNKK SSSSSSSSSS
     SSGKTGLFNK IFSGKDKQPS PQQAASPSTI DRTGQISTNR LEANVLSYSM SNLSKEVPLI
     TGDCVYCQGC NVILSRFSNL VKTGDDSFTW KCEFCKYSNS NILLEQGEIP NKDSVEYVLS
     SPSTSSTTDG SKREESIIIY CIDVSGSMGI TTEVPSLQSE WVNAKKGVKG ASSGPSYISR
     LECVQSSIPT MIDRLSIQYP NKRVVLVTFS DEVMIYTQSN SVDGPIVIAG DKLEDFDQLI
     EIGRSMTYDK LPTASGSSDF LKAKIKSLEP VQSTALGPAL LVSAAIASQK MLSEVVICTD
     GVPNVGLGAI EDLPLGPAQE FYEKVTKLAQ NNKTTINIIG ISGSHIDLGV IGKVSEQTNG
     NITIIHPLEL AREIRKLTQN PMIATDVEMS ICLHPTLEIN KYDSKQGLSR VVKQFPNVNS
     LTDLTLLYSS RNRPTEFVQI YPFQIQIKYT KLDGVRCLRV VSAQLQATPD FNTSTSNANI
     SILAMAFTQQ AAKIAQQQEY MESRLHLKAA TKLIRSLCNT DEQWEEFYNF EVLREEMEAP
     LITCIKNKQQ RVEKAATDDE IQVFYKMKNV HKSFVEGGRK KDISRRKGEA EINKQYYNIK
     FT