ID   CPCT_SYNP2              Reviewed;         199 AA.
AC   B1XI94; B0FYI2;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Phycocyanobilin lyase CpcT;
DE            EC=4.-.-.-;
GN   Name=cpcT; OrderedLocusNames=SYNPCC7002_A2095;
OS   Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum
OS   quadruplicatum).
OC   Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus;
OC   unclassified Synechococcus.
OX   NCBI_TaxID=32049;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN CHROMOPHORE ATTACHMENT, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RX   PubMed=16644722; DOI=10.1074/jbc.m602563200;
RA   Shen G., Saunee N.A., Williams S.R., Gallo E.F., Schluchter W.M.,
RA   Bryant D.A.;
RT   "Identification and characterization of a new class of bilin lyase: the
RT   cpcT gene encodes a bilin lyase responsible for attachment of
RT   phycocyanobilin to Cys-153 on the beta-subunit of phycocyanin in
RT   Synechococcus sp. PCC 7002.";
RL   J. Biol. Chem. 281:17768-17778(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RA   Li T., Zhao J., Zhao C., Liu Z., Zhao F., Marquardt J., Nomura C.T.,
RA   Persson S., Detter J.C., Richardson P.M., Lanz C., Schuster S.C., Wang J.,
RA   Li S., Huang X., Cai T., Yu Z., Luo J., Zhao J., Bryant D.A.;
RT   "Complete sequence of Synechococcus sp. PCC 7002.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Attaches a phycocyanobilin (PCB) chromophore to 'Cys-153' of
CC       the C-phycocyanin beta subunit (PhcB). It does not attach chromophore
CC       to CpcA, ApcA or ApcB, the other phycobiliproteins.
CC       {ECO:0000269|PubMed:16644722}.
CC   -!- DISRUPTION PHENOTYPE: Cells are a yellow color, 40% reduction in
CC       phycocyanin and grow slowly. The C-phycocyanin beta subunit (PhcB) in
CC       this strain is missing the phycocyanobilin chromophore on position 153.
CC       {ECO:0000269|PubMed:16644722}.
CC   -!- SIMILARITY: Belongs to the CpcT/CpeT biliprotein lyase family.
CC       {ECO:0000305}.
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DR   EMBL; EU364770; ABY63662.1; -; Genomic_DNA.
DR   EMBL; CP000951; ACB00079.1; -; Genomic_DNA.
DR   RefSeq; WP_012307700.1; NC_010475.1.
DR   AlphaFoldDB; B1XI94; -.
DR   SMR; B1XI94; -.
DR   STRING; 32049.SYNPCC7002_A2095; -.
DR   EnsemblBacteria; ACB00079; ACB00079; SYNPCC7002_A2095.
DR   KEGG; syp:SYNPCC7002_A2095; -.
DR   eggNOG; ENOG502Z877; Bacteria.
DR   HOGENOM; CLU_092589_0_0_3; -.
DR   OMA; NPPFFAH; -.
DR   BRENDA; 4.4.1.30; 6187.
DR   Proteomes; UP000001688; Chromosome.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017009; P:protein-phycocyanobilin linkage; IDA:UniProtKB.
DR   CDD; cd16338; CpcT; 1.
DR   Gene3D; 2.40.128.590; -; 1.
DR   HAMAP; MF_01460; Chrphore_lyase_CpxT; 1.
DR   InterPro; IPR010404; CpcT/CpeT.
DR   InterPro; IPR038672; CpcT/CpeT_sf.
DR   PANTHER; PTHR35137; PTHR35137; 1.
DR   Pfam; PF06206; CpeT; 1.
PE   1: Evidence at protein level;
KW   Lyase; Reference proteome.
FT   CHAIN           1..199
FT                   /note="Phycocyanobilin lyase CpcT"
FT                   /id="PRO_0000403163"
FT   CONFLICT        181
FT                   /note="A -> P (in Ref. 1; ABY63662)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   199 AA;  22483 MW;  AB3F5B6D35C343C4 CRC64;
     MSHSTDAHTL ARWMAGEFSN EAQALANPPL WAHIKVCMRP LPNQFFDGYG LYLEQAYSSD
     TSAPYRLRLF HIKPVDDHME LVHYKPKDDA KTKYMGAARN PAMMQHFDMA DLDPMPGCDM
     IVTWSGTSFK GTVQAGKGCR VVRYNKESYL DNSFEITDNA LISIDRGRDP VTNEILWGSL
     AGAFEFEKIN NFSGEVQPH