CPCU_SYNP2
ID CPCU_SYNP2 Reviewed; 183 AA.
AC A8HTN2;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Phycocyanobilin lyase subunit CpcU;
DE EC=4.-.-.-;
GN Name=cpcU; OrderedLocusNames=SYNPCC7002_A2053;
OS Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum
OS quadruplicatum).
OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus;
OC unclassified Synechococcus.
OX NCBI_TaxID=32049;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], POSSIBLE SUBUNIT, FUNCTION IN
RP CHROMOPHORE ATTACHMENT, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RX PubMed=18199754; DOI=10.1074/jbc.m708164200;
RA Shen G., Schluchter W.M., Bryant D.A.;
RT "Biogenesis of phycobiliproteins: I. cpcS-I and cpcU mutants of the
RT cyanobacterium Synechococcus sp. PCC 7002 define a heterodimeric
RT phyococyanobilin lyase specific for beta-phycocyanin and allophycocyanin
RT subunits.";
RL J. Biol. Chem. 283:7503-7512(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RA Li T., Zhao J., Zhao C., Liu Z., Zhao F., Marquardt J., Nomura C.T.,
RA Persson S., Detter J.C., Richardson P.M., Lanz C., Schuster S.C., Wang J.,
RA Li S., Huang X., Cai T., Yu Z., Luo J., Zhao J., Bryant D.A.;
RT "Complete sequence of Synechococcus sp. PCC 7002.";
RL Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION IN CHROMOPHORE ATTACHMENT, AND INTERACTION WITH CPCS.
RC STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RX PubMed=18199753; DOI=10.1074/jbc.m708165200;
RA Saunee N.A., Williams S.R., Bryant D.A., Schluchter W.M.;
RT "Biogenesis of phycobiliproteins: II. CpcS-I and CpcU comprise the
RT heterodimeric bilin lyase that attaches phycocyanobilin to Cys-82 of beta-
RT phycocyanin and CYS-81 of allophycocyanin subunits in Synechococcus sp. PCC
RT 7002.";
RL J. Biol. Chem. 283:7513-7522(2008).
RN [4]
RP PROTEIN SUBSTRATES, AND INTERACTION WITH CPCS.
RC STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RX PubMed=20228104; DOI=10.1128/aem.03100-09;
RA Biswas A., Vasquez Y.M., Dragomani T.M., Kronfel M.L., Williams S.R.,
RA Alvey R.M., Bryant D.A., Schluchter W.M.;
RT "Biosynthesis of cyanobacterial phycobiliproteins in Escherichia coli:
RT chromophorylation efficiency and specificity of all bilin lyases from
RT Synechococcus sp. strain PCC 7002.";
RL Appl. Environ. Microbiol. 76:2729-2739(2010).
CC -!- FUNCTION: Attaches a phycocyanobilin (PCB) chromophore to 'Cys-82' of
CC the C-phycocyanain beta subunit (PhcB) and the unique PCB that is
CC attached to allophycocyanin alpha and beta subunits (ApcA and ApcB).
CC {ECO:0000269|PubMed:18199753, ECO:0000269|PubMed:18199754}.
CC -!- SUBUNIT: Forms a heterodimer with CpcS.
CC -!- DISRUPTION PHENOTYPE: Cells are a yellow color, 70% reduction in
CC phycobiliproteins, grow slowly, have an increased sensitivity to high
CC light and a long lag phase. The C-phycocyanin beta subunit (PhcB) in
CC this strain is missing the phycocyanobilin chromophore on 'Cys-82'.
CC {ECO:0000269|PubMed:18199754}.
CC -!- SIMILARITY: Belongs to the CpcS/CpeS biliprotein lyase family.
CC {ECO:0000305}.
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DR EMBL; EU145732; ABV80285.1; -; Genomic_DNA.
DR EMBL; CP000951; ACB00040.1; -; Genomic_DNA.
DR RefSeq; WP_012307662.1; NC_010475.1.
DR AlphaFoldDB; A8HTN2; -.
DR SMR; A8HTN2; -.
DR STRING; 32049.SYNPCC7002_A2053; -.
DR EnsemblBacteria; ACB00040; ACB00040; SYNPCC7002_A2053.
DR KEGG; syp:SYNPCC7002_A2053; -.
DR eggNOG; ENOG50337PI; Bacteria.
DR HOGENOM; CLU_1651309_0_0_3; -.
DR OMA; NSVDWGK; -.
DR BRENDA; 4.4.1.29; 6187.
DR Proteomes; UP000001688; Chromosome.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0017009; P:protein-phycocyanobilin linkage; IEA:InterPro.
DR Gene3D; 2.40.128.20; -; 1.
DR HAMAP; MF_01459; Chrphore_lyase_CpxS; 1.
DR InterPro; IPR012674; Calycin.
DR InterPro; IPR018536; CpcS/CpeS.
DR Pfam; PF09367; CpeS; 1.
PE 1: Evidence at protein level;
KW Lyase; Reference proteome.
FT CHAIN 1..183
FT /note="Phycocyanobilin lyase subunit CpcU"
FT /id="PRO_0000403140"
SQ SEQUENCE 183 AA; 20596 MW; 80C99174D4BE1FD3 CRC64;
MDINAFIHQS AGNWFAQRTF YQAHHPEPDN GKANLAFELL PLDHPEVSRF AAAIAQAPNE
HWRVFQSSWD TSVDWGKPKA VGSSLFAFVI NPDQPTQGQA FSLDERTLAQ GQYRLGEDQI
LIVTLEAGEV KIIERQWFGN ENLRLRTNIV TGKTGVLQTA FYSEIRRIIE PEKTAEVTAE
ASN
