CPC_ARATH
ID CPC_ARATH Reviewed; 94 AA.
AC O22059;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Transcription factor CPC;
DE AltName: Full=Protein CAPRICE;
GN Name=CPC; OrderedLocusNames=At2g46410; ORFNames=F11C10.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=cv. Wassilewskija; TISSUE=Root;
RX PubMed=9262483; DOI=10.1126/science.277.5329.1113;
RA Wada T., Tachibana T., Shimura Y., Okada K.;
RT "Epidermal cell differentiation in Arabidopsis determined by a Myb homolog,
RT CPC.";
RL Science 277:1113-1116(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L.-J., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=12356720; DOI=10.1093/emboj/cdf524;
RA Schellmann S., Schnittger A., Kirik V., Wada T., Okada K., Beermann A.,
RA Thumfahrt J., Juergens G., Huelskamp M.;
RT "TRIPTYCHON and CAPRICE mediate lateral inhibition during trichome and root
RT hair patterning in Arabidopsis.";
RL EMBO J. 21:5036-5046(2002).
RN [7]
RP FUNCTION, AND INDUCTION.
RX PubMed=11910008; DOI=10.1105/tpc.010434;
RA Lee M.M., Schiefelbein J.;
RT "Cell pattern in the Arabidopsis root epidermis determined by lateral
RT inhibition with feedback.";
RL Plant Cell 14:611-618(2002).
RN [8]
RP INTERACTION WITH BHLH2.
RX PubMed=12917293; DOI=10.1242/dev.00681;
RA Zhang F., Gonzalez A., Zhao M., Payne C.T., Lloyd A.M.;
RT "A network of redundant bHLH proteins functions in all TTG1-dependent
RT pathways of Arabidopsis.";
RL Development 130:4859-4869(2003).
RN [9]
RP INTERACTION WITH GL3 AND BHLH2.
RX PubMed=14627722; DOI=10.1242/dev.00880;
RA Bernhardt C., Lee M.M., Gonzalez A., Zhang F., Lloyd A.M., Schiefelbein J.;
RT "The bHLH genes GLABRA3 (GL3) and ENHANCER OF GLABRA3 (EGL3) specify
RT epidermal cell fate in the Arabidopsis root.";
RL Development 130:6431-6439(2003).
RN [10]
RP INDUCTION BY WER.
RX PubMed=16207757; DOI=10.1242/dev.02055;
RA Ryu K.H., Kang Y.H., Park Y.-H., Hwang I., Schiefelbein J., Lee M.M.;
RT "The WEREWOLF MYB protein directly regulates CAPRICE transcription during
RT cell fate specification in the Arabidopsis root epidermis.";
RL Development 132:4765-4775(2005).
RN [11]
RP FUNCTION, SUBCELLULAR LOCATION, DOMAINS S1 AND S2 REGIONS, TISSUE
RP SPECIFICITY, AND MUTAGENESIS OF TRP-76 AND MET-78.
RX PubMed=16291794; DOI=10.1242/dev.02139;
RA Kurata T., Ishida T., Kawabata-Awai C., Noguchi M., Hattori S., Sano R.,
RA Nagasaka R., Tominaga R., Koshino-Kimura Y., Kato T., Sato S., Tabata S.,
RA Okada K., Wada T.;
RT "Cell-to-cell movement of the CAPRICE protein in Arabidopsis root epidermal
RT cell differentiation.";
RL Development 132:5387-5398(2005).
RN [12]
RP TISSUE SPECIFICITY, AUTOREGULATION, AND INDUCTION BY WER.
RX PubMed=15795220; DOI=10.1093/pcp/pci096;
RA Koshino-Kimura Y., Wada T., Tachibana T., Tsugeki R., Ishiguro S.,
RA Okada K.;
RT "Regulation of CAPRICE transcription by MYB proteins for root epidermis
RT differentiation in Arabidopsis.";
RL Plant Cell Physiol. 46:817-826(2005).
RN [13]
RP INDUCTION BY HDA18.
RX PubMed=16176989; DOI=10.1073/pnas.0503143102;
RA Xu C.-R., Liu C., Wang Y.-L., Li L.-C., Chen W.-Q., Xu Z.-H., Bai S.-N.;
RT "Histone acetylation affects expression of cellular patterning genes in the
RT Arabidopsis root epidermis.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:14469-14474(2005).
RN [14]
RP GENE FAMILY.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
RN [15]
RP INDUCTION.
RX PubMed=17450124; DOI=10.1038/nature05763;
RA Caro E., Castellano M.M., Gutierrez C.;
RT "A chromatin link that couples cell division to root epidermis patterning
RT in Arabidopsis.";
RL Nature 447:213-217(2007).
RN [16]
RP FUNCTION, AND INDUCTION.
RX PubMed=19513241; DOI=10.4161/psb.3.12.6254;
RA Serna L.;
RT "CAPRICE positively regulates stomatal formation in the Arabidopsis
RT hypocotyl.";
RL Plant Signal. Behav. 3:1077-1082(2008).
RN [17]
RP INTERACTION WITH SIEL.
RX PubMed=21924907; DOI=10.1016/j.cub.2011.08.013;
RA Koizumi K., Wu S., MacRae-Crerar A., Gallagher K.L.;
RT "An essential protein that interacts with endosomes and promotes movement
RT of the SHORT-ROOT transcription factor.";
RL Curr. Biol. 21:1559-1564(2011).
CC -!- FUNCTION: Transcription factor. Determines the fate of epidermal cell
CC differentiation. Represses trichome development by lateral inhibition.
CC Together with GL3 or BHLH2, promotes the formation of hair developing
CC cells (H position) in root epidermis, probably by inhibiting non-hair
CC cell formation. Represses the expression of GL2 and WER in H cells.
CC Positively regulates stomatal formation in the hypocotyl
CC (PubMed:19513241). {ECO:0000269|PubMed:11910008,
CC ECO:0000269|PubMed:12356720, ECO:0000269|PubMed:16291794,
CC ECO:0000269|PubMed:19513241, ECO:0000269|PubMed:9262483}.
CC -!- SUBUNIT: Interacts with GL3 and BHLH2. Interacts with SIEL
CC (PubMed:21924907). {ECO:0000269|PubMed:12917293,
CC ECO:0000269|PubMed:14627722, ECO:0000269|PubMed:21924907}.
CC -!- INTERACTION:
CC O22059; Q9CAD0: BHLH2; NbExp=3; IntAct=EBI-533386, EBI-533398;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16291794}. Note=Moves
CC from developing non-hair cells (atrichoblasts) to developing hair cells
CC (trichoblasts).
CC -!- TISSUE SPECIFICITY: Expressed in trichomes and in young developing
CC leaves, as well as in root hair and stele cells (pericycle and vascular
CC tissues). Expressed in epidermal root hairless cells (atrichoblasts)
CC and moves to root hair cells (trichoblasts) by a cell-to-cell movement
CC through plasmodesmata (at protein level). {ECO:0000269|PubMed:15795220,
CC ECO:0000269|PubMed:16291794}.
CC -!- DEVELOPMENTAL STAGE: Expressed in leaves primordia and later confined
CC to trichomes. {ECO:0000269|PubMed:12356720}.
CC -!- INDUCTION: Transcriptional repression correlates with reduced histone
CC acetylation on H3 and H4 mediated by HDA18 in root epidermis N cells
CC (non-hair developing cells). Induced by WER. Negative autoregulation by
CC interfering with the binding of WER to its WER-binding sites (WBS)
CC promoter region, especially in H cells. Down-regulated by GEM. Down-
CC regulated by TMM (PubMed:19513241). {ECO:0000269|PubMed:11910008,
CC ECO:0000269|PubMed:15795220, ECO:0000269|PubMed:16176989,
CC ECO:0000269|PubMed:16207757, ECO:0000269|PubMed:17450124,
CC ECO:0000269|PubMed:19513241}.
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DR EMBL; AB004871; BAA21917.1; -; mRNA.
DR EMBL; AY519521; AAS09991.1; -; mRNA.
DR EMBL; AC006526; AAD23043.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10691.1; -; Genomic_DNA.
DR EMBL; AY074637; AAL69453.1; -; mRNA.
DR PIR; E84902; E84902.
DR RefSeq; NP_182164.1; NM_130205.2.
DR AlphaFoldDB; O22059; -.
DR SMR; O22059; -.
DR BioGRID; 4584; 5.
DR IntAct; O22059; 5.
DR STRING; 3702.AT2G46410.1; -.
DR PaxDb; O22059; -.
DR PRIDE; O22059; -.
DR ProteomicsDB; 220410; -.
DR EnsemblPlants; AT2G46410.1; AT2G46410.1; AT2G46410.
DR GeneID; 819249; -.
DR Gramene; AT2G46410.1; AT2G46410.1; AT2G46410.
DR KEGG; ath:AT2G46410; -.
DR Araport; AT2G46410; -.
DR TAIR; locus:2005503; AT2G46410.
DR eggNOG; ENOG502S4DP; Eukaryota.
DR HOGENOM; CLU_178021_1_0_1; -.
DR InParanoid; O22059; -.
DR OMA; DGPLICA; -.
DR OrthoDB; 1531077at2759; -.
DR PhylomeDB; O22059; -.
DR PRO; PR:O22059; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22059; baseline and differential.
DR Genevisible; O22059; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0009913; P:epidermal cell differentiation; IMP:TAIR.
DR GO; GO:0010063; P:positive regulation of trichoblast fate specification; IMP:TAIR.
DR GO; GO:0010376; P:stomatal complex formation; IMP:UniProtKB.
DR CDD; cd00167; SANT; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 1.
DR SMART; SM00717; SANT; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..94
FT /note="Transcription factor CPC"
FT /id="PRO_0000234362"
FT DOMAIN 30..80
FT /note="Myb-like"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1..10
FT /note="S1, required for cell-to-cell movements"
FT REGION 76..79
FT /note="S2, required for cell-to-cell movements and nuclear
FT localization"
FT MUTAGEN 76
FT /note="W->A: Loss of cell-to-cell movement and of nuclear
FT localization."
FT /evidence="ECO:0000269|PubMed:16291794"
FT MUTAGEN 78
FT /note="M->A: Loss of cell-to-cell movement."
FT /evidence="ECO:0000269|PubMed:16291794"
SQ SEQUENCE 94 AA; 11385 MW; 608B1C7B4BA48846 CRC64;
MFRSDKAEKM DKRRRRQSKA KASCSEEVSS IEWEAVKMSE EEEDLISRMY KLVGDRWELI
AGRIPGRTPE EIERYWLMKH GVVFANRRRD FFRK
