CPDR_BRUA2
ID CPDR_BRUA2 Reviewed; 120 AA.
AC Q2YIF7;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 07-FEB-2006, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Response regulator receiver protein CpdR {ECO:0000305|PubMed:26124143};
DE AltName: Full=Phospho-receiver protein CpdR {ECO:0000303|PubMed:26124143};
GN Name=cpdR {ECO:0000303|PubMed:26124143};
GN OrderedLocusNames=BAB2_0042 {ECO:0000312|EMBL:CAJ12208.1};
OS Brucella abortus (strain 2308).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=359391;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2308;
RX PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL Infect. Immun. 73:8353-8361(2005).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION AT ASP-52, MUTAGENESIS OF
RP PHE-16 AND ASP-52, AND DISRUPTION PHENOTYPE.
RC STRAIN=2308;
RX PubMed=26124143; DOI=10.1073/pnas.1503118112;
RA Willett J.W., Herrou J., Briegel A., Rotskoff G., Crosson S.;
RT "Structural asymmetry in a conserved signaling system that regulates
RT division, replication, and virulence of an intracellular pathogen.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:E3709-E3718(2015).
CC -!- FUNCTION: Component of a regulatory phosphorelay system that controls
CC B.abortus cell growth, division, and intracellular survival inside
CC mammalian host cells. This signaling pathway is composed of CckA, ChpT,
CC CtrA and CpdR. CpdR is a response regulator substrate of ChpT.
CC Unphosphorylated CpdR controls steady-state levels of CtrA in the
CC B.abortus cell, likely via CtrA destabilization and activation of its
CC proteolysis. {ECO:0000269|PubMed:26124143}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:26124143}.
CC -!- PTM: Is phosphorylated by ChpT-P on Asp-52.
CC {ECO:0000269|PubMed:26124143}.
CC -!- DISRUPTION PHENOTYPE: Deletion of cpdR does not result in a gross
CC defect in cell morphology and has no effect on intracellular B.abortus
CC replication and/or survival in human macrophages.
CC {ECO:0000269|PubMed:26124143}.
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DR EMBL; AM040265; CAJ12208.1; -; Genomic_DNA.
DR RefSeq; WP_002966536.1; NZ_KN046823.1.
DR AlphaFoldDB; Q2YIF7; -.
DR SMR; Q2YIF7; -.
DR STRING; 359391.BAB2_0042; -.
DR EnsemblBacteria; CAJ12208; CAJ12208; BAB2_0042.
DR GeneID; 61314482; -.
DR KEGG; bmf:BAB2_0042; -.
DR PATRIC; fig|359391.11.peg.1993; -.
DR HOGENOM; CLU_000445_69_8_5; -.
DR OMA; LHSAPVC; -.
DR Proteomes; UP000002719; Chromosome II.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IDA:UniProtKB.
DR GO; GO:0031647; P:regulation of protein stability; IMP:UniProtKB.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR Pfam; PF00072; Response_reg; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..120
FT /note="Response regulator receiver protein CpdR"
FT /id="PRO_0000436620"
FT DOMAIN 3..117
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT MOD_RES 52
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169,
FT ECO:0000269|PubMed:26124143"
FT MUTAGEN 16
FT /note="F->R: 90% reduction in phosphoryl transfer from
FT CckA-P to CpdR via ChpT."
FT /evidence="ECO:0000269|PubMed:26124143"
FT MUTAGEN 52
FT /note="D->A: Cannot be phosphorylated. Leads to significant
FT reduction in CtrA protein level. Forms large elongated
FT cells with apparent budding at the midcell after 4 h of
FT induction, and after 24 h of induction the cells are highly
FT branched, which is consistent with a defect in proper cell
FT division. Significant reduction in intracellular B.abortus
FT replication and/or survival in human macrophages."
FT /evidence="ECO:0000269|PubMed:26124143"
SQ SEQUENCE 120 AA; 13586 MW; C9F89EC426BC75D3 CRC64;
MKRILLAEDD NDMRRFLVKA LEKAGYHVTH FDNGASAYER LQEEPFSLLL TDIVMPEMDG
IELARRATEI DPDLKIMFIT GFAAVALNPD SDAPRDAKVL SKPFHLRDLV NEIEKMLIAA
