2SS1_SESIN
ID 2SS1_SESIN Reviewed; 148 AA.
AC Q9XHP1;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=2S seed storage protein 1;
DE AltName: Full=2S albumin {ECO:0000303|PubMed:10606554, ECO:0000303|PubMed:12110835, ECO:0000303|PubMed:12842185};
DE AltName: Full=2S albumin storage protein;
DE AltName: Full=Allergen Ses i 2 {ECO:0000303|PubMed:12110835};
DE AltName: Full=Beta-globulin {ECO:0000303|PubMed:10606554, ECO:0000303|PubMed:15536424};
DE AltName: Allergen=Ses i 2.0101 {ECO:0000305};
DE Contains:
DE RecName: Full=2S seed storage protein 1 small subunit;
DE Contains:
DE RecName: Full=2S seed storage protein 1 large subunit;
DE Flags: Precursor;
OS Sesamum indicum (Oriental sesame) (Sesamum orientale).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Lamiales; Pedaliaceae; Sesamum.
OX NCBI_TaxID=4182;
RN [1] {ECO:0000312|EMBL:AAD42943.1}
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Tainan 1 {ECO:0000303|PubMed:10606554};
RC TISSUE=Seed {ECO:0000269|PubMed:10606554};
RX PubMed=10606554; DOI=10.1021/jf990366z;
RA Tai S.S.K., Wu L.S.H., Chen E.C.F., Tzen J.T.C.;
RT "Molecular cloning of 11S globulin and 2S albumin, the two major seed
RT storage proteins in sesame.";
RL J. Agric. Food Chem. 47:4932-4938(1999).
RN [2]
RP PROTEIN SEQUENCE OF 41-47; 53-59 AND 132-142, IDENTIFICATION BY MASS
RP SPECTROMETRY, TISSUE SPECIFICITY, ALLERGEN, AND REGION.
RX PubMed=12842185; DOI=10.1016/s0278-6915(03)00107-8;
RA Wolff N., Cogan U., Admon A., Dalal I., Katz Y., Hodos N., Karin N.,
RA Yannai S.;
RT "Allergy to sesame in humans is associated primarily with IgE antibody to a
RT 14 kDa 2S albumin precursor.";
RL Food Chem. Toxicol. 41:1165-1174(2003).
RN [3]
RP PROTEIN SEQUENCE OF 108-131, IDENTIFICATION BY MASS SPECTROMETRY, TISSUE
RP SPECIFICITY, AND ALLERGEN.
RX PubMed=12110835; DOI=10.1067/mai.2002.125487;
RA Beyer K., Bardina L., Grishina G., Sampson H.A.;
RT "Identification of sesame seed allergens by 2-dimensional proteomics and
RT Edman sequencing: seed storage proteins as common food allergens.";
RL J. Allergy Clin. Immunol. 110:154-159(2002).
RN [4]
RP ALLERGEN, AND REGIONS.
RX PubMed=15536424; DOI=10.1016/j.jaci.2004.07.038;
RA Wolff N., Yannai S., Karin N., Levy Y., Reifen R., Dalal I., Cogan U.;
RT "Identification and characterization of linear B-cell epitopes of beta-
RT globulin, a major allergen of sesame seeds.";
RL J. Allergy Clin. Immunol. 114:1151-1158(2004).
CC -!- FUNCTION: Seed storage protein. {ECO:0000305|PubMed:10606554,
CC ECO:0000305|PubMed:12110835, ECO:0000305|PubMed:12842185}.
CC -!- SUBUNIT: The mature protein consists of a small and a large chain
CC linked by disulfide bonds. {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in seeds (at protein level).
CC {ECO:0000269|PubMed:10606554, ECO:0000269|PubMed:12110835,
CC ECO:0000269|PubMed:12842185}.
CC -!- DEVELOPMENTAL STAGE: Expressed during seed maturation. Expressed in the
CC maturing seeds 24 days after flowering. {ECO:0000269|PubMed:10606554}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE of
CC patients allergic to sesame seeds (PubMed:12842185, PubMed:12110835,
CC PubMed:15536424). Binds to IgE in 92% of the 24 patients tested
CC (PubMed:12842185). Binds to IgE in 30% of the 20 patients tested
CC (PubMed:12110835). {ECO:0000269|PubMed:12110835,
CC ECO:0000269|PubMed:12842185, ECO:0000269|PubMed:15536424}.
CC -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC {ECO:0000255}.
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DR EMBL; AF091841; AAD42943.1; -; mRNA.
DR RefSeq; XP_011095387.1; XM_011097085.1.
DR AlphaFoldDB; Q9XHP1; -.
DR SMR; Q9XHP1; -.
DR Allergome; 3472; Ses i 2.0101.
DR Allergome; 625; Ses i 2.
DR EnsemblPlants; SIN_1014447.t; SIN_1014447.t.cds1; SIN_1014447.
DR GeneID; 105174860; -.
DR Gramene; SIN_1014447.t; SIN_1014447.t.cds1; SIN_1014447.
DR KEGG; sind:105174860; -.
DR OrthoDB; 1466684at2759; -.
DR PhylomeDB; Q9XHP1; -.
DR Proteomes; UP000504604; Linkage group LG12.
DR GO; GO:0042735; C:protein body; NAS:UniProtKB.
DR GO; GO:0019863; F:IgE binding; IEA:UniProtKB-KW.
DR GO; GO:0045735; F:nutrient reservoir activity; NAS:UniProtKB.
DR GO; GO:0010431; P:seed maturation; IEP:UniProtKB.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR000617; Napin/2SS/CON.
DR PANTHER; PTHR35496; PTHR35496; 1.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 1: Evidence at protein level;
KW Allergen; Direct protein sequencing; Disulfide bond; IgE-binding protein;
KW Reference proteome; Seed storage protein; Signal; Storage protein.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..38
FT /evidence="ECO:0000250|UniProtKB:P15457"
FT /id="PRO_0000043211"
FT CHAIN 39..68
FT /note="2S seed storage protein 1 small subunit"
FT /evidence="ECO:0000250|UniProtKB:P15457"
FT /id="PRO_0000043212"
FT PROPEP 69..76
FT /evidence="ECO:0000250|UniProtKB:P15457"
FT /id="PRO_0000043213"
FT CHAIN 77..146
FT /note="2S seed storage protein 1 large subunit"
FT /evidence="ECO:0000250|UniProtKB:P15457"
FT /id="PRO_0000043214"
FT PROPEP 147..148
FT /evidence="ECO:0000250|UniProtKB:P15457"
FT /id="PRO_0000043215"
FT REGION 24..94
FT /note="Involved in IgE-binding"
FT /evidence="ECO:0000269|PubMed:12842185"
FT REGION 46..55
FT /note="Immunodominant epitope; binds to IgE of 14 patients
FT out of 15 tested"
FT /evidence="ECO:0000269|PubMed:15536424"
FT REGION 48..57
FT /note="Immunodominant epitope; binds to IgE of 14 patients
FT out of 15 tested"
FT /evidence="ECO:0000269|PubMed:15536424"
FT REGION 76..86
FT /note="Immunodominant epitope; binds to IgE of 14 patients
FT out of 15 tested"
FT /evidence="ECO:0000269|PubMed:15536424"
FT DISULFID 42..97
FT /note="Interchain (between small and large chains)"
FT /evidence="ECO:0000250|UniProtKB:P04403"
FT DISULFID 54..86
FT /note="Interchain (between small and large chains)"
FT /evidence="ECO:0000250|UniProtKB:P04403"
FT DISULFID 87..133
FT /evidence="ECO:0000250|UniProtKB:P04403"
FT DISULFID 99..141
FT /evidence="ECO:0000250|UniProtKB:P04403"
SQ SEQUENCE 148 AA; 17524 MW; BA46B033BA13E3DE CRC64;
MARFTIVLAV LFAAALVSAS AHKTVVTTSV AEEGEEENQR GCEWESRQCQ MRHCMQWMRS
MRGQYEESFL RSAEANQGQF EHFRECCNEL RDVKSHCRCE ALRCMMRQMQ QEYGMEQEMQ
QMQQMMQYLP RMCGMSYPTE CRMRPIFA
