ID   CPGP1_ZINOF             Reviewed;         221 AA.
AC   P82473;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Zingipain-1;
DE            EC=3.4.22.67;
DE   AltName: Full=Cysteine proteinase GP-I;
OS   Zingiber officinale (Ginger) (Amomum zingiber).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Zingiberales; Zingiberaceae;
OC   Zingiber.
OX   NCBI_TaxID=94328;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Root;
RX   PubMed=10691991; DOI=10.1046/j.1432-1327.2000.01152.x;
RA   Choi K.H., Laursen R.A.;
RT   "Amino-acid sequence and glycan structures of cysteine proteases with
RT   proline specificity from ginger rhizome Zingiber officinale.";
RL   Eur. J. Biochem. 267:1516-1526(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Preferential cleavage of peptides with a proline residue at
CC         the P2 position.; EC=3.4.22.67;
CC   -!- SIMILARITY: Belongs to the peptidase C1 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU10090}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=Ref.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. May have been contaminated by a homologous protein.; Evidence={ECO:0000305};
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DR   PIR; A59040; A59040.
DR   MEROPS; C01.017; -.
DR   GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd02248; Peptidase_C1A; 1.
DR   InterPro; IPR038765; Papain-like_cys_pep_sf.
DR   InterPro; IPR025661; Pept_asp_AS.
DR   InterPro; IPR000668; Peptidase_C1A_C.
DR   InterPro; IPR039417; Peptidase_C1A_papain-like.
DR   Pfam; PF00112; Peptidase_C1; 1.
DR   SMART; SM00645; Pept_C1; 1.
DR   SUPFAM; SSF54001; SSF54001; 1.
DR   PROSITE; PS00640; THIOL_PROTEASE_ASN; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein; Hydrolase;
KW   Protease; Thiol protease.
FT   CHAIN           1..221
FT                   /note="Zingipain-1"
FT                   /id="PRO_0000050568"
FT   ACT_SITE        27
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10090"
FT   ACT_SITE        161
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10090"
FT   CARBOHYD        95
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        156
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        24..65
FT                   /evidence="ECO:0000250"
FT   DISULFID        58..98
FT                   /evidence="ECO:0000250"
FT   DISULFID        155..206
FT                   /evidence="ECO:0000250"
FT   UNSURE          2
FT                   /note="V or D"
SQ   SEQUENCE   221 AA;  24244 MW;  3035D7870EA743DB CRC64;
     DVLPDSIDWR EKGAVVPVKN QGGCGSCWAF DAIAAVEGIN QIVTGDLISL SEQQLVDCST
     RNHGCEGGWP YRAFQYIINN GGINSEEHYP YTGTNGTCDT KENAHVVSID SYRNVPSNDE
     KSLQKAVANQ PVSVTMDAAG RDFQLYRNGI FTGSCNISAN HYRTVGGRET ENDKDYWTVK
     NSWGKNWGES GYIRVERNIA ESSGKCGIAI SPSYPIKEXX X