ID   CPHB_GEMHP              Reviewed;         272 AA.
AC   P56946;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=Cyanophycinase;
DE            EC=3.4.15.6;
GN   Name=cphB;
OS   Geminocystis herdmanii (strain PCC 6308) (Synechocystis sp. (strain PCC
OS   6308)).
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC   Chroococcaceae; Geminocystis.
OX   NCBI_TaxID=113355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11131019; DOI=10.1007/s002030000206;
RA   Aboulmagd E., Oppermann-Sanio F.B., Steinbuechel A.;
RT   "Molecular characterization of the cyanophycin synthetase from
RT   Synechocystis sp. strain PCC6308.";
RL   Arch. Microbiol. 174:297-306(2000).
CC   -!- FUNCTION: Exopeptidase that catalyzes the hydrolytic cleavage of multi-
CC       L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve
CC       polymer) into aspartate-arginine dipeptides.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[L-4-(L-arginin-2-N-yl)aspartate](n) + H2O = [L-4-(L-arginin-
CC         2-N-yl)aspartate](n-1) + L-4-(L-arginin-2-N-yl)aspartate;
CC         Xref=Rhea:RHEA:12845, Rhea:RHEA-COMP:13728, Rhea:RHEA-COMP:13734,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:137986, ChEBI:CHEBI:137991;
CC         EC=3.4.15.6;
CC   -!- SIMILARITY: Belongs to the peptidase S51 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF220099; AAF43646.1; -; Genomic_DNA.
DR   AlphaFoldDB; P56946; -.
DR   SMR; P56946; -.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0044260; P:cellular macromolecule metabolic process; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.880; -; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR005320; Peptidase_S51.
DR   InterPro; IPR011811; Peptidase_S51_cyanophycinase.
DR   Pfam; PF03575; Peptidase_S51; 1.
DR   PIRSF; PIRSF032067; Cyanophycinase; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   TIGRFAMs; TIGR02069; cyanophycinase; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Serine protease.
FT   CHAIN           1..272
FT                   /note="Cyanophycinase"
FT                   /id="PRO_0000209975"
FT   ACT_SITE        132
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        150
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        174
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   272 AA;  29766 MW;  30A4A80E3068EC68 CRC64;
     MKQYLRSSVL VIGGAEDKVH GKEILQRFWH CAGGTDAIIA IIPSASREPT IIGDRYVSIF
     SEMGAKDLKV LDVRDRIQGE DKDYQEYVEK CTAIFMTGGD QLRLCGLLAD TPLMERIRQR
     VKLGEVTLGG TSAGAAVMGH HMIAGGSSGE SPNRALVDMA MGLGIIPEVI VDQHFHNRNR
     MARLLSALSN HPERLGIGID EDTCAVFQKD EYIEVIGKGT VTIVDGQAMS YTNHGKVAAE
     DPLALHNLRL HILGHGDRYN RKTHQPMAGI VE