ID   CPHN_EUPCP              Reviewed;          21 AA.
AC   C0HJB8;
DT   26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT   26-JUN-2013, sequence version 1.
DT   25-MAY-2022, entry version 7.
DE   RecName: Full=Cyanophlyctin {ECO:0000303|Ref.1};
OS   Euphlyctis cyanophlyctis (Skittering frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Dicroglossidae; Dicroglossinae;
OC   Euphlyctis.
OX   NCBI_TaxID=58519;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Skin secretion {ECO:0000269|Ref.1};
RX   DOI=10.1007/s10989-011-9284-6;
RA   Asoodeh A., Ghorani-Azam A., Chamani J.K.;
RT   "Identification and characterization of novel antibacterial peptides from
RT   skin secretions of Euphlyctis cyanophlyctis.";
RL   Int. J. Pept. Res. Ther. 18:107-115(2012).
CC   -!- FUNCTION: Has antibacterial activity against E.coli HP101BA (MIC=6.4
CC       uM), K.pneumoniae PTCC1388 (MIC=7.3 uM), M.luteus PTCC1625 (MIC=4.7 uM)
CC       and S.aureus PTCC1431 (MIC=5.3 uM). Has no or very limited (<3%)
CC       hemolytic activity at concentrations of 15 ug/ml and 60 ug/ml,
CC       respectively. {ECO:0000269|Ref.1}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands. {ECO:0000305|Ref.1}.
CC   -!- MASS SPECTROMETRY: Mass=2347.5; Mass_error=0.5; Method=MALDI;
CC       Evidence={ECO:0000269|Ref.1};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Brevinin subfamily. {ECO:0000255}.
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DR   AlphaFoldDB; C0HJB8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Secreted.
FT   PEPTIDE         1..21
FT                   /note="Cyanophlyctin"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="PRO_0000422917"
SQ   SEQUENCE   21 AA;  2348 MW;  D18159BAFC2396AD CRC64;
     FLNALKNFAK TAGKRLKSLL N