ID   CPI1_BRUMA              Reviewed;         127 AA.
AC   P90698; A0A0K0JBB7;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Cystatin cpi-1 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=cpi-1 {ECO:0000303|PubMed:9233676}; ORFNames=Bm1_20105;
OS   Brugia malayi (Filarial nematode worm).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Brugia.
OX   NCBI_TaxID=6279 {ECO:0000312|EMBL:AAC47623.1};
RN   [1] {ECO:0000312|EMBL:AAC47623.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX   PubMed=9233676; DOI=10.1016/s0166-6851(97)00050-9;
RA   Gregory W.F., Blaxter M.L., Maizels R.M.;
RT   "Differentially expressed, abundant trans-spliced cDNAs from larval Brugia
RT   malayi.";
RL   Mol. Biochem. Parasitol. 87:85-95(1997).
RN   [2] {ECO:0000312|EMBL:AAD51085.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Gregory W.F., Maizels R.M.;
RT   "Two distinct cystatin-type cysteine protease inhibitors from the parasitic
RT   nematode Brugia malayi.";
RL   Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000312|Proteomes:UP000006672}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FR3 {ECO:0000312|Proteomes:UP000006672};
RX   PubMed=17885136; DOI=10.1126/science.1145406;
RA   Ghedin E., Wang S., Spiro D., Caler E., Zhao Q., Crabtree J., Allen J.E.,
RA   Delcher A.L., Guiliano D.B., Miranda-Saavedra D., Angiuoli S.V., Creasy T.,
RA   Amedeo P., Haas B., El-Sayed N.M., Wortman J.R., Feldblyum T., Tallon L.,
RA   Schatz M., Shumway M., Koo H., Salzberg S.L., Schobel S., Pertea M.,
RA   Pop M., White O., Barton G.J., Carlow C.K.S., Crawford M.J., Daub J.,
RA   Dimmic M.W., Estes C.F., Foster J.M., Ganatra M., Gregory W.F.,
RA   Johnson N.M., Jin J., Komuniecki R., Korf I., Kumar S., Laney S., Li B.-W.,
RA   Li W., Lindblom T.H., Lustigman S., Ma D., Maina C.V., Martin D.M.,
RA   McCarter J.P., McReynolds L., Mitreva M., Nutman T.B., Parkinson J.,
RA   Peregrin-Alvarez J.M., Poole C., Ren Q., Saunders L., Sluder A.E.,
RA   Smith K., Stanke M., Unnasch T.R., Ware J., Wei A.D., Weil G.,
RA   Williams D.J., Zhang Y., Williams S.A., Fraser-Liggett C., Slatko B.,
RA   Blaxter M.L., Scott A.L.;
RT   "Draft genome of the filarial nematode parasite Brugia malayi.";
RL   Science 317:1756-1760(2007).
RN   [4] {ECO:0000305}
RP   FUNCTION.
RX   PubMed=15664654; DOI=10.1016/j.molbiopara.2004.11.008;
RA   Murray J., Manoury B., Balic A., Watts C., Maizels R.M.;
RT   "Bm-CPI-2, a cystatin from Brugia malayi nematode parasites, differs from
RT   Caenorhabditis elegans cystatins in a specific site mediating inhibition of
RT   the antigen-processing enzyme AEP.";
RL   Mol. Biochem. Parasitol. 139:197-203(2005).
RN   [5] {ECO:0000305}
RP   DEVELOPMENTAL STAGE.
RX   PubMed=18249028; DOI=10.1016/j.biocel.2007.11.012;
RA   Gregory W.F., Maizels R.M.;
RT   "Cystatins from filarial parasites: evolution, adaptation and function in
RT   the host-parasite relationship.";
RL   Int. J. Biochem. Cell Biol. 40:1389-1398(2008).
CC   -!- FUNCTION: Cysteine protease inhibitor which inhibits members of the
CC       peptidase C1 family (By similarity). Does not inhibit asparaginyl
CC       endopeptidase (PubMed:15664654). {ECO:0000250|UniProtKB:O16159,
CC       ECO:0000269|PubMed:15664654}.
CC   -!- DEVELOPMENTAL STAGE: In the vector stage, expression starts at the L2
CC       larval stage and continues throughout the L3 infective larval stage.
CC       After infecting the host, expression rapidly decreases and is absent
CC       from the L4 larval stage and in adults (PubMed:9233676,
CC       PubMed:18249028). Not expressed in microfilariae (PubMed:9233676).
CC       {ECO:0000269|PubMed:18249028, ECO:0000269|PubMed:9233676}.
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000255,
CC       ECO:0000255|RuleBase:RU362130}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U80972; AAC47623.1; -; mRNA.
DR   EMBL; AF177192; AAD51085.1; -; Genomic_DNA.
DR   EMBL; LN856902; CDP93728.1; -; Genomic_DNA.
DR   RefSeq; XP_001895476.1; XM_001895441.1.
DR   AlphaFoldDB; P90698; -.
DR   SMR; P90698; -.
DR   MEROPS; I25.043; -.
DR   EnsemblMetazoa; Bm367.1; Bm367.1; WBGene00220628.
DR   GeneID; 6098930; -.
DR   KEGG; bmy:BM_BM367; -.
DR   WBParaSite; Bm367.1; Bm367.1; WBGene00220628.
DR   CTD; 6098930; -.
DR   HOGENOM; CLU_118168_0_0_1; -.
DR   InParanoid; P90698; -.
DR   OMA; EVWSAPW; -.
DR   OrthoDB; 1565344at2759; -.
DR   Proteomes; UP000006672; Unassembled WGS sequence.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   Pfam; PF00031; Cystatin; 1.
DR   SMART; SM00043; CY; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Protease inhibitor; Reference proteome; Signal;
KW   Thiol protease inhibitor.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..127
FT                   /note="Cystatin cpi-1"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5013420733"
FT   MOTIF           68..72
FT                   /note="Secondary area of contact"
FT                   /evidence="ECO:0000305"
FT   DISULFID        86..98
FT                   /evidence="ECO:0000250|UniProtKB:P01038"
SQ   SEQUENCE   127 AA;  14612 MW;  AF59C346C7C23E67 CRC64;
     MFFPIVWLSV LLIISKSFAR EIRLSRATYN EDDEEIQEVA EKAMEQVNDQ TRYRNLYKLV
     RVISAQTQVV AGIKYYLTIL AAPTTCRKGA VGMNPMKCTI DSSKPTKQFK IEVWSAPWQN
     TFKVTLT