CPLA_DICDI
ID CPLA_DICDI Reviewed; 649 AA.
AC Q8MUF9; Q55BR8;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=Putative calpain-like cysteine protease A;
DE EC=3.4.22.-;
DE Contains:
DE RecName: Full=Putative processed calpain-like cysteine protease A;
DE Flags: Precursor;
GN Name=cplA; Synonyms=cpl; ORFNames=DDB_G0269200;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 4-15 AND 193-199, FUNCTION,
RP SUBCELLULAR LOCATION, SUBUNIT, AND AUTOCATALYTIC CLEAVAGE.
RC STRAIN=AX3;
RX PubMed=12578394; DOI=10.1021/bi026461+;
RA Huang X., Czerwinski E., Mellgren R.L.;
RT "Purification and properties of the Dictyostelium calpain-like protein,
RT Cpl.";
RL Biochemistry 42:1789-1795(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Has a weak caseinolytic activity.
CC {ECO:0000269|PubMed:12578394}.
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:12578394}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:12578394}.
CC -!- PTM: Undergoes autolytic cleavage between Pro-192 and Ala-193.
CC -!- SIMILARITY: Belongs to the peptidase C2 family. {ECO:0000305}.
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DR EMBL; AF525418; AAM91927.1; -; mRNA.
DR EMBL; AAFI02000005; EAL71950.1; -; Genomic_DNA.
DR RefSeq; XP_646763.1; XM_641671.1.
DR AlphaFoldDB; Q8MUF9; -.
DR SMR; Q8MUF9; -.
DR STRING; 44689.DDB0191171; -.
DR PaxDb; Q8MUF9; -.
DR PRIDE; Q8MUF9; -.
DR EnsemblProtists; EAL71950; EAL71950; DDB_G0269200.
DR GeneID; 8617736; -.
DR KEGG; ddi:DDB_G0269200; -.
DR dictyBase; DDB_G0269200; cplA.
DR eggNOG; ENOG502SS2N; Eukaryota.
DR HOGENOM; CLU_422394_0_0_1; -.
DR InParanoid; Q8MUF9; -.
DR OMA; TMASDNI; -.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR PRO; PR:Q8MUF9; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IDA:dictyBase.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR Gene3D; 2.60.40.150; -; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR022684; Calpain_cysteine_protease.
DR InterPro; IPR022682; Calpain_domain_III.
DR InterPro; IPR022683; Calpain_III.
DR InterPro; IPR036213; Calpain_III_sf.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF01067; Calpain_III; 2.
DR PRINTS; PR00704; CALPAIN.
DR SMART; SM00720; calpain_III; 2.
DR SUPFAM; SSF49562; SSF49562; 1.
DR SUPFAM; SSF49758; SSF49758; 3.
DR PROSITE; PS50004; C2; 1.
PE 1: Evidence at protein level;
KW Autocatalytic cleavage; Cytoplasm; Direct protein sequencing; Hydrolase;
KW Protease; Reference proteome; Repeat; Thiol protease.
FT PROPEP 1..3
FT /evidence="ECO:0000269|PubMed:12578394"
FT /id="PRO_0000327882"
FT CHAIN 4..649
FT /note="Putative calpain-like cysteine protease A"
FT /id="PRO_0000327883"
FT CHAIN 193..649
FT /note="Putative processed calpain-like cysteine protease A"
FT /id="PRO_0000327884"
FT DOMAIN 15..123
FT /note="C2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 124..193
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 458..489
FT /note="Domain III 1"
FT REGION 498..633
FT /note="Domain III 2"
FT COMPBIAS 137..157
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 158..175
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 192..193
FT /note="Cleavage; by autolysis"
SQ SEQUENCE 649 AA; 72623 MW; 1D1691E255288570 CRC64;
MLTTESPTTT TTTTTTTTSS PSSDIRDKFI FGNLEVKVKE VKGCQLHFLN VKCELALKKT
EIKTKPLANH TFFDVFSFRV SAATSELEIE AWKKNFLFKD KMTGSLTIPI NDLLHANGEA
KWYPLSNKKP RSSRVKKENI TNSNNKDNNT ASPSSPDEAQ EKGDEDQHHS ADESPAEETS
PTTGRPRSVS MPAKKVKAAP EICLEIKFVL NEPPKEVLKG IVLDGVWNSE NNFGSLINNP
HWIKCTQYLL SIKDEITPIT LKLRQPEGTD QRCSFFVINY DPFYNGSKKV ILDTTNDIKK
VSSFNSPIPA TSVDCKIDLE PGQYCIIPYA ESFAFSGTYK FNLDSEKLDN CEFYALPKSQ
EQAWNEITVD GLWTTATNGG GDINILGWTK NPQYSFTLTK KSRACVLLSQ DDNDKSVGFY
VIKQLDAGKR AIEFREQVGK TESFKFSCST GCTLTLDEGT YIVIPSTYDH GIEGAFHLTL
FTDDKNATFQ PLTNAFQEVE QVKGTWVGKS AGGSPNQPTF FNNPQFHLKV PASDKDEVIA
VQLIQDSTIA DEGIGFIVLS RDSHSEPLTA QDFQNEMVFT KTSNWEKRND IVCRLHVKPE
SPREFTIIPS TFDPSVNRSF KLQVYSDVSI SLDEIEQKDE SSDSEQNDD
