ID   CPLN2_XENLA             Reviewed;         249 AA.
AC   Q6GNL4; A0A1L8ER78;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Ciliogenesis and planar polarity effector 2 {ECO:0000305};
DE   AltName: Full=REM2- and Rab-like small GTPase 1 {ECO:0000303|PubMed:19767740};
GN   Name=cplane2 {ECO:0000303|PubMed:19767740}; Synonyms=rsg1;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1] {ECO:0000312|Proteomes:UP000186698}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=J {ECO:0000312|Proteomes:UP000186698};
RX   PubMed=27762356; DOI=10.1038/nature19840;
RA   Session A.M., Uno Y., Kwon T., Chapman J.A., Toyoda A., Takahashi S.,
RA   Fukui A., Hikosaka A., Suzuki A., Kondo M., van Heeringen S.J., Quigley I.,
RA   Heinz S., Ogino H., Ochi H., Hellsten U., Lyons J.B., Simakov O.,
RA   Putnam N., Stites J., Kuroki Y., Tanaka T., Michiue T., Watanabe M.,
RA   Bogdanovic O., Lister R., Georgiou G., Paranjpe S.S., van Kruijsbergen I.,
RA   Shu S., Carlson J., Kinoshita T., Ohta Y., Mawaribuchi S., Jenkins J.,
RA   Grimwood J., Schmutz J., Mitros T., Mozaffari S.V., Suzuki Y., Haramoto Y.,
RA   Yamamoto T.S., Takagi C., Heald R., Miller K., Haudenschild C., Kitzman J.,
RA   Nakayama T., Izutsu Y., Robert J., Fortriede J., Burns K., Lotay V.,
RA   Karimi K., Yasuoka Y., Dichmann D.S., Flajnik M.F., Houston D.W.,
RA   Shendure J., DuPasquier L., Vize P.D., Zorn A.M., Ito M., Marcotte E.M.,
RA   Wallingford J.B., Ito Y., Asashima M., Ueno N., Matsuda Y., Veenstra G.J.,
RA   Fujiyama A., Harland R.M., Taira M., Rokhsar D.S.;
RT   "Genome evolution in the allotetraploid frog Xenopus laevis.";
RL   Nature 538:336-343(2016).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, INTERACTION WITH FUZ, MUTAGENESIS OF THR-65, AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=19767740; DOI=10.1038/ncb1966;
RA   Gray R.S., Abitua P.B., Wlodarczyk B.J., Szabo-Rogers H.L., Blanchard O.,
RA   Lee I., Weiss G.S., Liu K.J., Marcotte E.M., Wallingford J.B.,
RA   Finnell R.H.;
RT   "The planar cell polarity effector Fuz is essential for targeted membrane
RT   trafficking, ciliogenesis and mouse embryonic development.";
RL   Nat. Cell Biol. 11:1225-1232(2009).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=27158779; DOI=10.1038/ng.3558;
RA   Toriyama M., Lee C., Taylor S.P., Duran I., Cohn D.H., Bruel A.L.,
RA   Tabler J.M., Drew K., Kelly M.R., Kim S., Park T.J., Braun D.A.,
RA   Pierquin G., Biver A., Wagner K., Malfroot A., Panigrahi I., Franco B.,
RA   Al-Lami H.A., Yeung Y., Choi Y.J., Duffourd Y., Faivre L., Riviere J.B.,
RA   Chen J., Liu K.J., Marcotte E.M., Hildebrandt F., Thauvin-Robinet C.,
RA   Krakow D., Jackson P.K., Wallingford J.B.;
RT   "The ciliopathy-associated CPLANE proteins direct basal body recruitment of
RT   intraflagellar transport machinery.";
RL   Nat. Genet. 48:648-656(2016).
CC   -!- FUNCTION: Potential effector of the planar cell polarity signaling
CC       pathway. Plays a role in targeted membrane trafficking most probably at
CC       the level of vesicle fusion with membranes. Involved in cilium
CC       biogenesis by regulating the transport of cargo proteins to the basal
CC       body and to the apical tips of cilia. More generally involved in
CC       exocytosis in secretory cells. {ECO:0000269|PubMed:19767740}.
CC   -!- SUBUNIT: Interacts with fuz. {ECO:0000269|PubMed:19767740}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body
CC       {ECO:0000269|PubMed:19767740, ECO:0000269|PubMed:27158779}.
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC       {ECO:0000305}.
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DR   EMBL; CM004483; OCT61847.1; -; Genomic_DNA.
DR   EMBL; BC073493; AAH73493.1; -; mRNA.
DR   RefSeq; NP_001085897.1; NM_001092428.1.
DR   AlphaFoldDB; Q6GNL4; -.
DR   SMR; Q6GNL4; -.
DR   IntAct; Q6GNL4; 1.
DR   STRING; 8355.A0A1L8ER78; -.
DR   PRIDE; Q6GNL4; -.
DR   DNASU; 444324; -.
DR   GeneID; 444324; -.
DR   KEGG; xla:444324; -.
DR   CTD; 444324; -.
DR   Xenbase; XB-GENE-5914970; cplane2l.S.
DR   OMA; LWHQDCV; -.
DR   OrthoDB; 1324082at2759; -.
DR   Proteomes; UP000186698; Chromosome 9_10S.
DR   Bgee; 444324; Expressed in oocyte and 20 other tissues.
DR   GO; GO:0036064; C:ciliary basal body; IDA:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0060271; P:cilium assembly; IMP:UniProtKB.
DR   GO; GO:0006887; P:exocytosis; IEA:UniProtKB-KW.
DR   GO; GO:0001843; P:neural tube closure; IMP:UniProtKB.
DR   GO; GO:0008104; P:protein localization; IMP:UniProtKB.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0017157; P:regulation of exocytosis; IMP:UniProtKB.
DR   GO; GO:0031338; P:regulation of vesicle fusion; IMP:UniProtKB.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR039677; RSG1.
DR   InterPro; IPR001806; Small_GTPase.
DR   PANTHER; PTHR14983; PTHR14983; 1.
DR   Pfam; PF00071; Ras; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   Cell projection; Cilium; Cilium biogenesis/degradation; Cytoplasm;
KW   Cytoskeleton; Exocytosis; GTP-binding; Nucleotide-binding;
KW   Protein transport; Reference proteome; Transport.
FT   CHAIN           1..249
FT                   /note="Ciliogenesis and planar polarity effector 2"
FT                   /id="PRO_0000284541"
FT   REGION          46..249
FT                   /note="Small GTPase-like"
FT   BINDING         58..65
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         171..174
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   MUTAGEN         65
FT                   /note="T->N: Loss of localization to the cilium basal body.
FT                   Dominant negative form altering cilium biogenesis."
FT                   /evidence="ECO:0000269|PubMed:19767740"
SQ   SEQUENCE   249 AA;  27867 MW;  F5DA2393D475BA3E CRC64;
     MSVTPVLDPE WQRSPEGLDY LSRVLRHNKR KFFGLIERPV LPPHLPADVA AYKVFVCGKS
     GVGKTSFIAK LSGLAVPSMH HETAGIQTTC MYWPVRPSGS ARPVIFRFQF WDCGEGALRK
     FDHILPACKE KADAVLFLFS FTDRSSFEDV PALISRTLDQ DEDVTRVVIG TKLDQYMHTD
     VTEDDLRDFQ RTWQLPVMRV RSVNGPRMTD GRDLDGRAGL AECAPVLNGL AEILWHRDQV
     IAGLVGGAE