CPLX2_HUMAN
ID CPLX2_HUMAN Reviewed; 134 AA.
AC Q6PUV4; B2RAG2; O09056; Q13329; Q28184; Q52M15; Q64386;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Complexin-2;
DE AltName: Full=Complexin II;
DE Short=CPX II;
DE AltName: Full=Synaphin-1;
GN Name=CPLX2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=7553862; DOI=10.1016/0092-8674(95)90239-2;
RA McMahon H.T., Missler M., Li C., Suedhof T.C.;
RT "Complexins: cytosolic proteins that regulate SNAP receptor function.";
RL Cell 83:111-119(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-114, AND TISSUE SPECIFICITY.
RC TISSUE=Cerebellum;
RX PubMed=16162394; DOI=10.1016/j.gene.2005.07.005;
RA Raevskaya N.M., Dergunova L.V., Vladychenskaya I.P., Stavchansky V.V.,
RA Oborina M.V., Poltaraus A.B., Limborska S.A.;
RT "Structural organization of the human complexin 2 gene (CPLX2) and aspects
RT of its functional activity.";
RL Gene 359:127-137(2005).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=9853440; DOI=10.1016/s0140-6736(98)03341-8;
RA Harrison P.J., Eastwood S.L.;
RT "Preferential involvement of excitatory neurons in medial temporal lobe in
RT schizophrenia.";
RL Lancet 352:1669-1673(1998).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=11576753; DOI=10.1016/s0361-9230(01)00530-5;
RA Eastwood S.L., Harrison P.J.;
RT "Synaptic pathology in the anterior cingulate cortex in schizophrenia and
RT mood disorders. A review and a Western blot study of synaptophysin, GAP-43
RT and the complexins.";
RL Brain Res. Bull. 55:569-578(2001).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=11483314; DOI=10.1016/s0306-4522(01)00141-5;
RA Eastwood S.L., Cotter D., Harrison P.J.;
RT "Cerebellar synaptic protein expression in schizophrenia.";
RL Neuroscience 105:219-229(2001).
RN [8]
RP TISSUE SPECIFICITY.
RX PubMed=15906159; DOI=10.1007/s11068-004-0514-8;
RA DiProspero N.A., Chen E.-Y., Charles V., Plomann M., Kordower J.H.,
RA Tagle D.A.;
RT "Early changes in Huntington's disease patient brains involve alterations
RT in cytoskeletal and synaptic elements.";
RL J. Neurocytol. 33:517-533(2004).
CC -!- FUNCTION: Negatively regulates the formation of synaptic vesicle
CC clustering at active zone to the presynaptic membrane in postmitotic
CC neurons. Positively regulates a late step in exocytosis of various
CC cytoplasmic vesicles, such as synaptic vesicles and other secretory
CC vesicles. Also involved in mast cell exocytosis (By similarity).
CC {ECO:0000250|UniProtKB:P84086, ECO:0000250|UniProtKB:P84088}.
CC -!- SUBUNIT: Binds to the SNARE core complex containing SNAP25, VAMP2 and
CC STX1A. {ECO:0000250}.
CC -!- INTERACTION:
CC Q6PUV4; Q9HB07: MYG1; NbExp=3; IntAct=EBI-2689453, EBI-709754;
CC Q6PUV4; Q9ULP0-2: NDRG4; NbExp=3; IntAct=EBI-2689453, EBI-11978907;
CC Q6PUV4; Q96AL5: PBX3; NbExp=3; IntAct=EBI-2689453, EBI-741171;
CC Q6PUV4; P43897: TSFM; NbExp=3; IntAct=EBI-2689453, EBI-1049298;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:P84087}. Presynapse
CC {ECO:0000250|UniProtKB:P84087}. Nucleus {ECO:0000250|UniProtKB:P84087}.
CC Perikaryon {ECO:0000250|UniProtKB:P84087}. Note=Translocated from the
CC perikaryon to the presynaptic terminals during maturation of neuronal
CC cells. In mast cells, cytosol and nucleus. Becomes enriched near plasma
CC membrane following stimulation. {ECO:0000250|UniProtKB:P84087}.
CC -!- TISSUE SPECIFICITY: Nervous system. In hippocampus and cerebellum,
CC expressed mainly by excitatory neurons. Down-regulated in brain cortex
CC from patients suffering from Huntington disease, bipolar disorder or
CC major depression. Down-regulated in cerebellum from patients with
CC schizophrenia. {ECO:0000269|PubMed:11483314,
CC ECO:0000269|PubMed:11576753, ECO:0000269|PubMed:15906159,
CC ECO:0000269|PubMed:16162394, ECO:0000269|PubMed:9853440}.
CC -!- SIMILARITY: Belongs to the complexin/synaphin family. {ECO:0000305}.
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DR EMBL; U35100; AAC50229.1; -; mRNA.
DR EMBL; AK314177; BAG36859.1; -; mRNA.
DR EMBL; BC093706; AAH93706.1; -; mRNA.
DR EMBL; BC112287; AAI12288.1; -; mRNA.
DR EMBL; AY576870; AAS93622.1; -; mRNA.
DR EMBL; BN000499; CAG26663.1; -; mRNA.
DR EMBL; BN000500; CAG26664.1; -; mRNA.
DR CCDS; CCDS4396.1; -.
DR PIR; E57233; E57233.
DR RefSeq; NP_001008221.1; NM_001008220.1.
DR RefSeq; NP_006641.1; NM_006650.3.
DR RefSeq; XP_005265855.1; XM_005265798.1.
DR RefSeq; XP_005265856.1; XM_005265799.1.
DR RefSeq; XP_011532721.1; XM_011534419.1.
DR RefSeq; XP_016864453.1; XM_017008964.1.
DR AlphaFoldDB; Q6PUV4; -.
DR SMR; Q6PUV4; -.
DR BioGRID; 116027; 23.
DR CORUM; Q6PUV4; -.
DR IntAct; Q6PUV4; 14.
DR MINT; Q6PUV4; -.
DR STRING; 9606.ENSP00000352544; -.
DR iPTMnet; Q6PUV4; -.
DR PhosphoSitePlus; Q6PUV4; -.
DR BioMuta; CPLX2; -.
DR DMDM; 51316998; -.
DR jPOST; Q6PUV4; -.
DR MassIVE; Q6PUV4; -.
DR MaxQB; Q6PUV4; -.
DR PaxDb; Q6PUV4; -.
DR PeptideAtlas; Q6PUV4; -.
DR PRIDE; Q6PUV4; -.
DR ProteomicsDB; 67260; -.
DR TopDownProteomics; Q6PUV4; -.
DR Antibodypedia; 45968; 182 antibodies from 26 providers.
DR DNASU; 10814; -.
DR Ensembl; ENST00000359546.8; ENSP00000352544.4; ENSG00000145920.15.
DR Ensembl; ENST00000393745.8; ENSP00000377346.3; ENSG00000145920.15.
DR Ensembl; ENST00000515094.1; ENSP00000421825.1; ENSG00000145920.15.
DR GeneID; 10814; -.
DR KEGG; hsa:10814; -.
DR MANE-Select; ENST00000393745.8; ENSP00000377346.3; NM_001008220.2; NP_001008221.1.
DR UCSC; uc003mde.2; human.
DR CTD; 10814; -.
DR DisGeNET; 10814; -.
DR GeneCards; CPLX2; -.
DR HGNC; HGNC:2310; CPLX2.
DR HPA; ENSG00000145920; Tissue enhanced (brain, epididymis, pituitary gland).
DR MIM; 605033; gene.
DR neXtProt; NX_Q6PUV4; -.
DR OpenTargets; ENSG00000145920; -.
DR PharmGKB; PA26827; -.
DR VEuPathDB; HostDB:ENSG00000145920; -.
DR eggNOG; ENOG502RXXI; Eukaryota.
DR GeneTree; ENSGT00950000182938; -.
DR HOGENOM; CLU_132159_1_0_1; -.
DR InParanoid; Q6PUV4; -.
DR OMA; KHTRMEA; -.
DR PhylomeDB; Q6PUV4; -.
DR TreeFam; TF315172; -.
DR PathwayCommons; Q6PUV4; -.
DR SignaLink; Q6PUV4; -.
DR BioGRID-ORCS; 10814; 16 hits in 1065 CRISPR screens.
DR ChiTaRS; CPLX2; human.
DR GeneWiki; CPLX2; -.
DR GenomeRNAi; 10814; -.
DR Pharos; Q6PUV4; Tbio.
DR PRO; PR:Q6PUV4; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q6PUV4; protein.
DR Bgee; ENSG00000145920; Expressed in right hemisphere of cerebellum and 134 other tissues.
DR ExpressionAtlas; Q6PUV4; baseline and differential.
DR Genevisible; Q6PUV4; HS.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0043204; C:perikaryon; IEA:UniProtKB-SubCell.
DR GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR GO; GO:0070554; C:synaptobrevin 2-SNAP-25-syntaxin-3-complexin complex; TAS:ParkinsonsUK-UCL.
DR GO; GO:0043195; C:terminal bouton; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0019905; F:syntaxin binding; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0043303; P:mast cell degranulation; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0031915; P:positive regulation of synaptic plasticity; ISS:UniProtKB.
DR GO; GO:0017157; P:regulation of exocytosis; TAS:ParkinsonsUK-UCL.
DR GO; GO:0046928; P:regulation of neurotransmitter secretion; IBA:GO_Central.
DR GO; GO:0016079; P:synaptic vesicle exocytosis; IBA:GO_Central.
DR GO; GO:0006904; P:vesicle docking involved in exocytosis; TAS:ProtInc.
DR InterPro; IPR008849; Synaphin.
DR PANTHER; PTHR16705; PTHR16705; 1.
DR Pfam; PF05835; Synaphin; 1.
PE 1: Evidence at protein level;
KW Cell projection; Coiled coil; Cytoplasm; Differentiation; Exocytosis;
KW Mast cell degranulation; Neurogenesis; Neurotransmitter transport; Nucleus;
KW Phosphoprotein; Reference proteome; Synapse; Transport.
FT CHAIN 1..134
FT /note="Complexin-2"
FT /id="PRO_0000144875"
FT REGION 1..114
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 41..97
FT /note="Interaction with the SNARE complex"
FT /evidence="ECO:0000250"
FT COILED 28..84
FT /evidence="ECO:0000255"
FT COMPBIAS 18..86
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 93
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P84087"
SQ SEQUENCE 134 AA; 15394 MW; 7791812FD4194AC5 CRC64;
MDFVMKQALG GATKDMGKML GGEEEKDPDA QKKEEERQEA LRQQEEERKA KHARMEAERE
KVRQQIRDKY GLKKKEEKEA EEKAALEQPC EGSLTRPKKA IPAGCGDEEE EEEESILDTV
LKYLPGPLQD MFKK
