CPLX_DROME
ID CPLX_DROME Reviewed; 142 AA.
AC Q8IPM8; A4V2D0; A4V2D3; A8JQS1; A8JQS4; A8JQS8; A8JQS9; Q6NL98; Q86P65;
AC Q8IPM7; Q8IPM9; Q8MRE4; Q9NBA0;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Complexin;
DE Flags: Precursor;
GN Name=cpx; ORFNames=CG32490;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM E).
RX PubMed=10777504; DOI=10.1074/jbc.m002571200;
RA Pabst S., Hazzard J.W., Antonin W., Suedhof T.C., Jahn R., Rizo J.,
RA Fasshauer D.;
RT "Selective interaction of complexin with the neuronal SNARE complex.
RT Determination of the binding regions.";
RL J. Biol. Chem. 275:19808-19818(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A; C AND J).
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Positively regulates a late step in synaptic vesicle
CC exocytosis. {ECO:0000250}.
CC -!- SUBUNIT: Binds to the SNARE core complex containing Snap25,
CC synaptobrevin and Syx1A. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=7;
CC Name=A; Synonyms=G, H, I;
CC IsoId=Q8IPM8-1; Sequence=Displayed;
CC Name=E;
CC IsoId=Q8IPM8-2; Sequence=VSP_019325, VSP_019326;
CC Name=C; Synonyms=M, N, O, P;
CC IsoId=Q8IPM8-3; Sequence=VSP_019324;
CC Name=J; Synonyms=K;
CC IsoId=Q8IPM8-4; Sequence=VSP_019323;
CC Name=L;
CC IsoId=Q8IPM8-5; Sequence=VSP_035401;
CC Name=R;
CC IsoId=Q8IPM8-7; Sequence=VSP_035402;
CC Name=T;
CC IsoId=Q8IPM8-8; Sequence=VSP_041846;
CC -!- SIMILARITY: Belongs to the complexin/synaphin family. {ECO:0000305}.
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DR EMBL; AF260578; AAF69518.1; -; mRNA.
DR EMBL; AE014297; AAN13291.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13292.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13293.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13294.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13295.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13296.2; -; Genomic_DNA.
DR EMBL; AE014297; AAX52938.1; -; Genomic_DNA.
DR EMBL; AE014297; AAX52939.1; -; Genomic_DNA.
DR EMBL; AE014297; ABW08589.1; -; Genomic_DNA.
DR EMBL; AE014297; ABW08590.1; -; Genomic_DNA.
DR EMBL; AE014297; ABW08591.1; -; Genomic_DNA.
DR EMBL; AE014297; ABW08592.1; -; Genomic_DNA.
DR EMBL; AE014297; ABW08593.1; -; Genomic_DNA.
DR EMBL; AE014297; ABW08594.2; -; Genomic_DNA.
DR EMBL; AE014297; ABW08595.1; -; Genomic_DNA.
DR EMBL; AY121629; AAM51956.1; -; mRNA.
DR EMBL; BT001797; AAN71552.1; -; mRNA.
DR EMBL; BT003456; AAO39459.1; -; mRNA.
DR EMBL; BT012438; AAS93709.1; -; mRNA.
DR RefSeq; NP_001014602.1; NM_001014602.3. [Q8IPM8-4]
DR RefSeq; NP_001014603.1; NM_001014603.3. [Q8IPM8-4]
DR RefSeq; NP_001097673.1; NM_001104203.3. [Q8IPM8-5]
DR RefSeq; NP_001097674.1; NM_001104204.3. [Q8IPM8-3]
DR RefSeq; NP_001097676.1; NM_001104206.3. [Q8IPM8-3]
DR RefSeq; NP_001097677.1; NM_001104207.2. [Q8IPM8-3]
DR RefSeq; NP_001097678.3; NM_001104208.4.
DR RefSeq; NP_001163500.1; NM_001170029.2.
DR RefSeq; NP_001163501.1; NM_001170030.1. [Q8IPM8-1]
DR RefSeq; NP_001163502.1; NM_001170031.2. [Q8IPM8-3]
DR RefSeq; NP_001163503.1; NM_001170032.2. [Q8IPM8-1]
DR RefSeq; NP_001163504.1; NM_001170033.2.
DR RefSeq; NP_525019.2; NM_080280.4. [Q8IPM8-2]
DR RefSeq; NP_730800.1; NM_164327.5. [Q8IPM8-1]
DR RefSeq; NP_730801.1; NM_164328.4. [Q8IPM8-1]
DR RefSeq; NP_730802.1; NM_164329.3. [Q8IPM8-1]
DR RefSeq; NP_730803.1; NM_164330.4. [Q8IPM8-3]
DR RefSeq; NP_730804.2; NM_164331.3. [Q8IPM8-1]
DR AlphaFoldDB; Q8IPM8; -.
DR BioGRID; 72863; 8.
DR DIP; DIP-29220N; -.
DR IntAct; Q8IPM8; 33.
DR STRING; 7227.FBpp0290013; -.
DR PaxDb; Q8IPM8; -.
DR DNASU; 64877; -.
DR EnsemblMetazoa; FBtr0078897; FBpp0078537; FBgn0041605. [Q8IPM8-1]
DR EnsemblMetazoa; FBtr0078898; FBpp0078538; FBgn0041605. [Q8IPM8-1]
DR EnsemblMetazoa; FBtr0078899; FBpp0078539; FBgn0041605. [Q8IPM8-1]
DR EnsemblMetazoa; FBtr0078900; FBpp0078540; FBgn0041605. [Q8IPM8-3]
DR EnsemblMetazoa; FBtr0078901; FBpp0078541; FBgn0041605. [Q8IPM8-1]
DR EnsemblMetazoa; FBtr0078902; FBpp0078542; FBgn0041605. [Q8IPM8-2]
DR EnsemblMetazoa; FBtr0100499; FBpp0099936; FBgn0041605. [Q8IPM8-4]
DR EnsemblMetazoa; FBtr0100500; FBpp0099938; FBgn0041605. [Q8IPM8-4]
DR EnsemblMetazoa; FBtr0113337; FBpp0112249; FBgn0041605. [Q8IPM8-5]
DR EnsemblMetazoa; FBtr0113338; FBpp0112250; FBgn0041605. [Q8IPM8-3]
DR EnsemblMetazoa; FBtr0113340; FBpp0112252; FBgn0041605. [Q8IPM8-3]
DR EnsemblMetazoa; FBtr0113341; FBpp0112253; FBgn0041605. [Q8IPM8-3]
DR EnsemblMetazoa; FBtr0301769; FBpp0290983; FBgn0041605. [Q8IPM8-1]
DR EnsemblMetazoa; FBtr0301770; FBpp0290984; FBgn0041605. [Q8IPM8-3]
DR EnsemblMetazoa; FBtr0301771; FBpp0290985; FBgn0041605. [Q8IPM8-1]
DR GeneID; 64877; -.
DR KEGG; dme:Dmel_CG32490; -.
DR UCSC; CG32490-RA; d. melanogaster. [Q8IPM8-1]
DR UCSC; CG32490-RL; d. melanogaster.
DR UCSC; CG32490-RM; d. melanogaster.
DR UCSC; CG32490-RQ; d. melanogaster.
DR UCSC; CG32490-RR; d. melanogaster.
DR CTD; 64877; -.
DR FlyBase; FBgn0041605; cpx.
DR VEuPathDB; VectorBase:FBgn0041605; -.
DR eggNOG; ENOG502S3I2; Eukaryota.
DR GeneTree; ENSGT00950000182938; -.
DR HOGENOM; CLU_132159_0_1_1; -.
DR InParanoid; Q8IPM8; -.
DR PhylomeDB; Q8IPM8; -.
DR Reactome; R-DME-181429; Serotonin Neurotransmitter Release Cycle.
DR Reactome; R-DME-181430; Norepinephrine Neurotransmitter Release Cycle.
DR Reactome; R-DME-210500; Glutamate Neurotransmitter Release Cycle.
DR Reactome; R-DME-212676; Dopamine Neurotransmitter Release Cycle.
DR Reactome; R-DME-264642; Acetylcholine Neurotransmitter Release Cycle.
DR Reactome; R-DME-888590; GABA synthesis, release, reuptake and degradation.
DR BioGRID-ORCS; 64877; 0 hits in 3 CRISPR screens.
DR ChiTaRS; cpx; fly.
DR GenomeRNAi; 64877; -.
DR PRO; PR:Q8IPM8; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0041605; Expressed in brain and 21 other tissues.
DR ExpressionAtlas; Q8IPM8; baseline and differential.
DR Genevisible; Q8IPM8; DM.
DR GO; GO:0005829; C:cytosol; HDA:FlyBase.
DR GO; GO:0016020; C:membrane; IDA:FlyBase.
DR GO; GO:0031594; C:neuromuscular junction; IDA:FlyBase.
DR GO; GO:0048786; C:presynaptic active zone; IDA:FlyBase.
DR GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR GO; GO:0043195; C:terminal bouton; IDA:FlyBase.
DR GO; GO:0061174; C:type I terminal bouton; IDA:FlyBase.
DR GO; GO:0005326; F:neurotransmitter transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0019905; F:syntaxin binding; IEA:InterPro.
DR GO; GO:0050804; P:modulation of chemical synaptic transmission; IMP:FlyBase.
DR GO; GO:0046929; P:negative regulation of neurotransmitter secretion; IMP:FlyBase.
DR GO; GO:0007274; P:neuromuscular synaptic transmission; IMP:FlyBase.
DR GO; GO:0046928; P:regulation of neurotransmitter secretion; IMP:FlyBase.
DR GO; GO:0051124; P:synaptic assembly at neuromuscular junction; IMP:FlyBase.
DR GO; GO:0016079; P:synaptic vesicle exocytosis; IMP:FlyBase.
DR InterPro; IPR008849; Synaphin.
DR PANTHER; PTHR16705; PTHR16705; 1.
DR Pfam; PF05835; Synaphin; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Exocytosis; Lipoprotein; Membrane;
KW Methylation; Neurotransmitter transport; Prenylation; Reference proteome;
KW Transport.
FT CHAIN 1..139
FT /note="Complexin"
FT /id="PRO_0000240243"
FT PROPEP 140..142
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000240244"
FT REGION 13..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 83..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 29..138
FT /evidence="ECO:0000255"
FT COMPBIAS 24..70
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 139
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000255"
FT LIPID 139
FT /note="S-farnesyl cysteine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..59
FT /note="Missing (in isoform J)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_019323"
FT VAR_SEQ 2..19
FT /note="AAFIAKQMVGNQLSAVKG -> RNRLITSRRSLIYSKAGILTQRS (in
FT isoform T)"
FT /evidence="ECO:0000305"
FT /id="VSP_041846"
FT VAR_SEQ 2..18
FT /note="AAFIAKQMVGNQLSAVK -> NFI (in isoform L)"
FT /evidence="ECO:0000305"
FT /id="VSP_035401"
FT VAR_SEQ 3..19
FT /note="AFIAKQMVGNQLSAVKG -> LHIYFKVHRFC (in isoform R)"
FT /evidence="ECO:0000305"
FT /id="VSP_035402"
FT VAR_SEQ 19..21
FT /note="Missing (in isoform C)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_019324"
FT VAR_SEQ 117
FT /note="F -> FT (in isoform E)"
FT /evidence="ECO:0000303|PubMed:10777504"
FT /id="VSP_019325"
FT VAR_SEQ 122..142
FT /note="NQIETQVNELKTQIEGKCVMQ -> KRLSDAFKNCPLRNLF (in
FT isoform E)"
FT /evidence="ECO:0000303|PubMed:10777504"
FT /id="VSP_019326"
FT CONFLICT 19..21
FT /note="GAV -> DAAG (in Ref. 4; AAM51956/AAN71552)"
FT /evidence="ECO:0000305"
FT CONFLICT 124
FT /note="I -> M (in Ref. 4; AAS93709)"
FT /evidence="ECO:0000305"
FT CONFLICT 129
FT /note="N -> S (in Ref. 4; AAS93709)"
FT /evidence="ECO:0000305"
FT CONFLICT Q8IPM8-2:126
FT /note="S -> N (in Ref. 4; AAS93709)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 142 AA; 16416 MW; 6836C3AF3BF64F19 CRC64;
MAAFIAKQMV GNQLSAVKGA VGGDGGDDGD DKEKAEEEER ERQEAIKEAE DRRKEKHRKM
EEEREKMRQD IRDKYNIKKK EEIVEAAPQE EPNPLMRKKK TPEELAAEAE QEELDDFTKL
KNQIETQVNE LKTQIEGKCV MQ
