ID   CPNA_COMTE              Reviewed;         250 AA.
AC   Q937L4;
DT   29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Cyclopentanol dehydrogenase {ECO:0000250|UniProtKB:Q8GAV9};
DE            EC=1.1.1.163 {ECO:0000250|UniProtKB:Q8GAV9};
DE   AltName: Full=Cyclohexanol dehydrogenase {ECO:0000303|PubMed:12588297};
DE            EC=1.1.1.245 {ECO:0000269|PubMed:12588297};
GN   Name=cpnA {ECO:0000303|PubMed:12588297}; Synonyms=cpmB;
OS   Comamonas testosteroni (Pseudomonas testosteroni).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Comamonas.
OX   NCBI_TaxID=285;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=12588297; DOI=10.1046/j.1462-2920.2003.00401.x;
RA   van Beilen J.B., Mourlane F., Seeger M.A., Kovac J., Li Z., Smits T.H.M.,
RA   Fritsche U., Witholt B.;
RT   "Cloning of Baeyer-Villiger monooxygenases from Comamonas, Xanthobacter and
RT   Rhodococcus via PCR with highly degenerate primers.";
RL   Environ. Microbiol. 5:174-182(2003).
CC   -!- FUNCTION: Catalyzes the oxidation of cyclopentanol to cyclopentanone
CC       and cyclohexanol to cyclohexanone. {ECO:0000250|UniProtKB:Q8GAV9,
CC       ECO:0000269|PubMed:12588297}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cyclopentanol + NAD(+) = cyclopentanone + H(+) + NADH;
CC         Xref=Rhea:RHEA:11728, ChEBI:CHEBI:15378, ChEBI:CHEBI:16133,
CC         ChEBI:CHEBI:16486, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC         EC=1.1.1.163; Evidence={ECO:0000250|UniProtKB:Q8GAV9};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cyclohexanol + NAD(+) = cyclohexanone + H(+) + NADH;
CC         Xref=Rhea:RHEA:10044, ChEBI:CHEBI:15378, ChEBI:CHEBI:17854,
CC         ChEBI:CHEBI:18099, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC         EC=1.1.1.245; Evidence={ECO:0000269|PubMed:12588297};
CC   -!- PATHWAY: Alcohol metabolism; cyclopentanol degradation; 5-valerolactone
CC       from cyclopentanol: step 1/2.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; AJ418060; CAD10799.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q937L4; -.
DR   SMR; Q937L4; -.
DR   UniPathway; UPA00764; UER00749.
DR   GO; GO:0018460; F:cyclohexanol dehydrogenase activity; IEA:RHEA.
DR   GO; GO:0055041; F:cyclopentanol dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0033022; P:cyclopentanol catabolic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   NAD; Oxidoreductase.
FT   CHAIN           1..250
FT                   /note="Cyclopentanol dehydrogenase"
FT                   /id="PRO_0000054552"
FT   ACT_SITE        155
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         16..18
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         37
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         63..64
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         90
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         155
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         159
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         185..193
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
SQ   SEQUENCE   250 AA;  26626 MW;  7C42C3CBAEBB858E CRC64;
     MGRVNDKVVL VTGGAMGMGL THCTLLAREG ATVYLSDMNE ELGHQAVAEI RRQGGKAHFL
     HLDVTNENHW TGAVDTILAE SDRLDALVNN AGILTLKPVQ DTSNEEWDRI FEINVRSVFL
     GTRAVIEPMR KAHKGCIVNV SSIYGLVGAP GAAAYEASKG AVRLFTKACA VDLAPFNIRV
     NSVHPGVIAT PMTQQILDAP QSARALLGPT LLGRAAQPME VSQAVLFLVS DEASFVHGSE
     LVVDGGYTAN