CPNB_DICDI
ID CPNB_DICDI Reviewed; 530 AA.
AC Q86K21; Q556H8;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Copine-B;
GN Name=cpnB-1; ORFNames=DDB_G0272875;
GN and
GN Name=cpnB-2; ORFNames=DDB_G0274051;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=16343335; DOI=10.1186/1471-2121-6-46;
RA Damer C.K., Bayeva M., Hahn E.S., Rivera J., Socec C.I.;
RT "Copine A, a calcium-dependent membrane-binding protein, transiently
RT localizes to the plasma membrane and intracellular vacuoles in
RT Dictyostelium.";
RL BMC Cell Biol. 6:46-46(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=17259548; DOI=10.1128/ec.00322-06;
RA Damer C.K., Bayeva M., Kim P.S., Ho L.K., Eberhardt E.S., Socec C.I.,
RA Lee J.S., Bruce E.A., Goldman-Yassen A.E., Naliboff L.C.;
RT "Copine A is required for cytokinesis, contractile vacuole function, and
RT development in Dictyostelium.";
RL Eukaryot. Cell 6:430-442(2007).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00041};
CC -!- DEVELOPMENTAL STAGE: Expressed at relatively high levels in vegetative
CC cells. The expression goes down at the 2th hour of development and
CC increase slightly at the 8th hour time point.
CC {ECO:0000269|PubMed:17259548}.
CC -!- SIMILARITY: Belongs to the copine family. {ECO:0000305}.
CC -!- CAUTION: The gene for this protein is duplicated in strains AX3 and
CC AX4. These strains contain a duplication of a segment of 750 kb of
CC chromosome 2 compared to the corresponding sequence in strain AX2.
CC {ECO:0000305}.
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DR EMBL; AY593970; AAT02416.1; -; mRNA.
DR EMBL; AAFI02000011; EAL70459.1; -; Genomic_DNA.
DR EMBL; AAFI02000009; EAL71075.1; -; Genomic_DNA.
DR RefSeq; XP_644384.1; XM_639292.1.
DR RefSeq; XP_645029.1; XM_639937.1.
DR AlphaFoldDB; Q86K21; -.
DR SMR; Q86K21; -.
DR PaxDb; Q86K21; -.
DR EnsemblProtists; EAL70459; EAL70459; DDB_G0274051.
DR EnsemblProtists; EAL71075; EAL71075; DDB_G0272875.
DR GeneID; 8618706; -.
DR GeneID; 8619270; -.
DR KEGG; ddi:DDB_G0272875; -.
DR KEGG; ddi:DDB_G0274051; -.
DR dictyBase; DDB_G0272875; cpnB-1.
DR dictyBase; DDB_G0274051; cpnB-2.
DR eggNOG; KOG1327; Eukaryota.
DR InParanoid; Q86K21; -.
DR PhylomeDB; Q86K21; -.
DR Reactome; R-DDI-1483206; Glycerophospholipid biosynthesis.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR PRO; PR:Q86K21; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:dictyBase.
DR GO; GO:0005544; F:calcium-dependent phospholipid binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0071277; P:cellular response to calcium ion; IDA:dictyBase.
DR CDD; cd04047; C2B_Copine; 1.
DR CDD; cd01459; vWA_copine_like; 1.
DR Gene3D; 2.60.40.150; -; 2.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR037768; C2B_Copine.
DR InterPro; IPR045052; Copine.
DR InterPro; IPR010734; Copine_C.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR10857; PTHR10857; 1.
DR Pfam; PF00168; C2; 2.
DR Pfam; PF07002; Copine; 1.
DR SMART; SM00239; C2; 2.
DR SMART; SM00327; VWA; 1.
DR SUPFAM; SSF49562; SSF49562; 2.
DR SUPFAM; SSF53300; SSF53300; 1.
DR PROSITE; PS50004; C2; 2.
DR PROSITE; PS50234; VWFA; 1.
PE 2: Evidence at transcript level;
KW Calcium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..530
FT /note="Copine-B"
FT /id="PRO_0000330655"
FT DOMAIN 1..125
FT /note="C2 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 130..253
FT /note="C2 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 294..513
FT /note="VWFA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT BINDING 25
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 25
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 31
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 85
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 85
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 87
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 87
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 100
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT CONFLICT 25..28
FT /note="DICS -> GKYQY (in Ref. 3; EAL70459/EAL71075)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 530 AA; 58830 MW; C2607CDA01F90566 CRC64;
MTTPISLSKP RVELRFKCSH LKNLDICSKS DPLIMVFDKR GEHGESIFVG QTEKINNNLN
PEFKKSVIID YHFENIQNLS FIVVDIDKEI KRVGDLEGND IIGQYTTTLG NIISKPNKKV
ISEIKHKGKE TGVIEITAEE IRETGHNFLF KINGTKLDKK DLFTSDPYFK IYKTSSSGNV
LVYQSVVIKN TLNPNYEPVM MKLEELNNGD MFRELVFEFW DHDSVGEHDF IGLFTTNADT
VLKGITREFP LINAKKAAKK SGSYKNSGVI TFTDCRLVGQ PTFIDYLSGG CEINLMVAID
CTASNGSPST STSLHYHTPS HPSQYAKSIF SVGSVLAPYD SDGNIEVLGF GGIHRGSTSH
CFQFGSKKEV RGVEGVLLTY GEVIPSMSLS YPTNFKDIIS YAAKKSLDGV TSKSQKYTIL
LILTDGEISD MNETVHEIVK ASKQSPLSIV IIGIGEATFD NMNRLDGDDG NSLTDSSGQT
ATRDIVQFVP FNNFSANPEA LATETLREIP QQLLGFMNQN NYLPMSHRLK
