CPNE4_HUMAN
ID CPNE4_HUMAN Reviewed; 557 AA.
AC Q96A23; D3DNC5; Q8TEX1;
DT 28-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Copine-4 {ECO:0000305};
DE AltName: Full=Copine IV {ECO:0000250|UniProtKB:Q99829, ECO:0000312|HGNC:HGNC:2317};
DE AltName: Full=Copine-8 {ECO:0000303|PubMed:12670487};
GN Name=CPNE4 {ECO:0000312|HGNC:HGNC:2317};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND TISSUE SPECIFICITY.
RC TISSUE=Prostate;
RX PubMed=12670487; DOI=10.1016/s0006-291x(03)00445-5;
RA Maitra R., Grigoryev D.N., Bera T.K., Pastan I.H., Lee B.;
RT "Cloning, molecular characterization, and expression analysis of copine
RT 8.";
RL Biochem. Biophys. Res. Commun. 303:842-847(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP INTERACTION WITH ACTB; BCOR; BICD2; CCDC22; CDC42BPB; CEP162; MYCBP2; NONO;
RP PDCD6; PITPNM2; RDX; SKIL; SKT; SPTBN1; UBE2O AND WTAP.
RX PubMed=12522145; DOI=10.1074/jbc.m212632200;
RA Tomsig J.L., Snyder S.L., Creutz C.E.;
RT "Identification of targets for calcium signaling through the copine family
RT of proteins. Characterization of a coiled-coil copine-binding motif.";
RL J. Biol. Chem. 278:10048-10054(2003).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=12949241; DOI=10.1189/jlb.0203083;
RA Cowland J.B., Carter D., Bjerregaard M.D., Johnsen A.H., Borregaard N.,
RA Lollike K.;
RT "Tissue expression of copines and isolation of copines I and III from the
RT cytosol of human neutrophils.";
RL J. Leukoc. Biol. 74:379-388(2003).
CC -!- FUNCTION: Probable calcium-dependent phospholipid-binding protein that
CC may play a role in calcium-mediated intracellular processes.
CC {ECO:0000250|UniProtKB:Q99829}.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00041};
CC -!- SUBUNIT: Interacts (via VWFA domain) with ACTB, BCOR, BICD2, CCDC22,
CC CDC42BPB, CEP162, MYCBP2, NONO, PDCD6, PITPNM2, RDX, SKIL, SKT, SPTBN1,
CC UBE2O and WTAP (PubMed:12522145). {ECO:0000269|PubMed:12522145}.
CC -!- INTERACTION:
CC Q96A23-2; Q8N987: NECAB1; NbExp=6; IntAct=EBI-12302039, EBI-11956853;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q96A23-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96A23-2; Sequence=VSP_001214;
CC -!- TISSUE SPECIFICITY: Widely expressed (PubMed:12670487,
CC PubMed:12949241). Expressed strongly in the brain, heart and prostate
CC (PubMed:12670487, PubMed:12949241). Expressed strongly in peripheral
CC blood leukocytes (PubMed:12949241). {ECO:0000269|PubMed:12670487,
CC ECO:0000269|PubMed:12949241}.
CC -!- SIMILARITY: Belongs to the copine family. {ECO:0000305}.
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DR EMBL; AF465770; AAL74190.1; -; mRNA.
DR EMBL; AF465771; AAL74191.1; -; mRNA.
DR EMBL; AK056175; BAB71111.1; -; mRNA.
DR EMBL; CH471052; EAW79206.1; -; Genomic_DNA.
DR EMBL; CH471052; EAW79207.1; -; Genomic_DNA.
DR EMBL; BC014396; AAH14396.1; -; mRNA.
DR EMBL; BC075046; AAH75046.1; -; mRNA.
DR CCDS; CCDS3072.1; -. [Q96A23-1]
DR CCDS; CCDS75010.1; -. [Q96A23-2]
DR RefSeq; NP_001276041.1; NM_001289112.1. [Q96A23-2]
DR RefSeq; NP_570720.1; NM_130808.2. [Q96A23-1]
DR RefSeq; NP_702907.1; NM_153429.1. [Q96A23-2]
DR RefSeq; XP_016861182.1; XM_017005693.1.
DR AlphaFoldDB; Q96A23; -.
DR SMR; Q96A23; -.
DR BioGRID; 126267; 51.
DR IntAct; Q96A23; 23.
DR STRING; 9606.ENSP00000478878; -.
DR GlyGen; Q96A23; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q96A23; -.
DR PhosphoSitePlus; Q96A23; -.
DR BioMuta; CPNE4; -.
DR DMDM; 26006702; -.
DR EPD; Q96A23; -.
DR jPOST; Q96A23; -.
DR MassIVE; Q96A23; -.
DR MaxQB; Q96A23; -.
DR PaxDb; Q96A23; -.
DR PeptideAtlas; Q96A23; -.
DR PRIDE; Q96A23; -.
DR ProteomicsDB; 75894; -. [Q96A23-1]
DR ProteomicsDB; 75895; -. [Q96A23-2]
DR TopDownProteomics; Q96A23-1; -. [Q96A23-1]
DR Antibodypedia; 33351; 88 antibodies from 19 providers.
DR DNASU; 131034; -.
DR Ensembl; ENST00000429747.6; ENSP00000411904.1; ENSG00000196353.13. [Q96A23-1]
DR Ensembl; ENST00000502818.5; ENSP00000421646.1; ENSG00000196353.13. [Q96A23-2]
DR Ensembl; ENST00000511604.5; ENSP00000423811.1; ENSG00000196353.13. [Q96A23-1]
DR Ensembl; ENST00000512055.5; ENSP00000421705.1; ENSG00000196353.13. [Q96A23-1]
DR Ensembl; ENST00000512332.5; ENSP00000424853.1; ENSG00000196353.13. [Q96A23-2]
DR Ensembl; ENST00000617767.5; ENSP00000478878.1; ENSG00000196353.13. [Q96A23-2]
DR GeneID; 131034; -.
DR KEGG; hsa:131034; -.
DR MANE-Select; ENST00000429747.6; ENSP00000411904.1; NM_130808.3; NP_570720.1.
DR UCSC; uc003eok.5; human. [Q96A23-1]
DR CTD; 131034; -.
DR DisGeNET; 131034; -.
DR GeneCards; CPNE4; -.
DR HGNC; HGNC:2317; CPNE4.
DR HPA; ENSG00000196353; Group enriched (brain, prostate).
DR MIM; 604208; gene.
DR neXtProt; NX_Q96A23; -.
DR OpenTargets; ENSG00000196353; -.
DR PharmGKB; PA26834; -.
DR VEuPathDB; HostDB:ENSG00000196353; -.
DR eggNOG; KOG1327; Eukaryota.
DR GeneTree; ENSGT00940000155519; -.
DR HOGENOM; CLU_020452_4_0_1; -.
DR InParanoid; Q96A23; -.
DR OMA; XVIAEEL; -.
DR OrthoDB; 1067545at2759; -.
DR PhylomeDB; Q96A23; -.
DR TreeFam; TF316419; -.
DR PathwayCommons; Q96A23; -.
DR SignaLink; Q96A23; -.
DR BioGRID-ORCS; 131034; 8 hits in 1069 CRISPR screens.
DR ChiTaRS; CPNE4; human.
DR GeneWiki; CPNE4; -.
DR GenomeRNAi; 131034; -.
DR Pharos; Q96A23; Tbio.
DR PRO; PR:Q96A23; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q96A23; protein.
DR Bgee; ENSG00000196353; Expressed in lateral nuclear group of thalamus and 129 other tissues.
DR ExpressionAtlas; Q96A23; baseline and differential.
DR Genevisible; Q96A23; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005544; F:calcium-dependent phospholipid binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0071277; P:cellular response to calcium ion; IBA:GO_Central.
DR CDD; cd04047; C2B_Copine; 1.
DR CDD; cd01459; vWA_copine_like; 1.
DR Gene3D; 2.60.40.150; -; 2.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR037768; C2B_Copine.
DR InterPro; IPR045052; Copine.
DR InterPro; IPR010734; Copine_C.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR10857; PTHR10857; 1.
DR Pfam; PF00168; C2; 2.
DR Pfam; PF07002; Copine; 1.
DR SMART; SM00239; C2; 2.
DR SMART; SM00327; VWA; 1.
DR SUPFAM; SSF49562; SSF49562; 2.
DR SUPFAM; SSF53300; SSF53300; 1.
DR PROSITE; PS50004; C2; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Calcium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..557
FT /note="Copine-4"
FT /id="PRO_0000144841"
FT DOMAIN 3..131
FT /note="C2 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 137..264
FT /note="C2 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 305..507
FT /note="VWFA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT BINDING 170
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 170
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 176
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 232
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 232
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 234
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 234
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 240
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT VAR_SEQ 1
FT /note="M -> MAVEPSSSESIKRRAGRRM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12670487"
FT /id="VSP_001214"
SQ SEQUENCE 557 AA; 62395 MW; 34AA5F103FE3C7BD CRC64;
MKKMSNIYES AANTLGIFNS PCLTKVELRV ACKGISDRDA LSKPDPCVIL KMQSHGQWFE
VDRTEVIRTC INPVYSKLFT VDFYFEEVQR LRFEVHDISS NHNGLKEADF LGGMECTLGQ
IVSQRKLSKS LLKHGNTAGK SSITVIAEEL SGNDDYVELA FNARKLDDKD FFSKSDPFLE
IFRMNDDATQ QLVHRTEVVM NNLSPAWKSF KVSVNSLCSG DPDRRLKCIV WDWDSNGKHD
FIGEFTSTFK EMRGAMEGKQ VQWECINPKY KAKKKNYKNS GTVILNLCKI HKMHSFLDYI
MGGCQIQFTV AIDFTASNGD PRNSCSLHYI HPYQPNEYLK ALVAVGEICQ DYDSDKMFPA
FGFGARIPPE YTVSHDFAIN FNEDNPECAG IQGVVEAYQS CLPKLQLYGP TNIAPIIQKV
AKSASEETNT KEASQYFILL ILTDGVITDM ADTREAIVHA SHLPMSVIIV GVGNADFSDM
QMLDGDDGIL RSPKGEPVLR DIVQFVPFRN FKHASPAALA KSVLAEVPNQ VVDYYNGKGI
KPKCSSEMYE SSRTLAP
