CPNE4_MOUSE
ID CPNE4_MOUSE Reviewed; 557 AA.
AC Q8BLR2;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Copine-4 {ECO:0000305};
DE AltName: Full=Copine IV {ECO:0000250|UniProtKB:Q99829, ECO:0000312|MGI:MGI:1921270};
GN Name=Cpne4 {ECO:0000312|MGI:MGI:1921270};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain cortex;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Probable calcium-dependent phospholipid-binding protein that
CC may play a role in calcium-mediated intracellular processes.
CC {ECO:0000250|UniProtKB:Q99829}.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00041};
CC -!- SUBUNIT: Interacts (via VWFA domain) with ACTB, BCOR, BICD2, CCDC22,
CC CDC42BPB, CEP162, MYCBP2, NONO, PDCD6, PITPNM2, RDX, SKIL, SKT, SPTBN1,
CC UBE2O and WTAP. {ECO:0000250|UniProtKB:Q96A23}.
CC -!- SIMILARITY: Belongs to the copine family. {ECO:0000305}.
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DR EMBL; AK043692; BAC31619.1; -; mRNA.
DR EMBL; BC043087; AAH43087.1; -; mRNA.
DR EMBL; BC063081; AAH63081.1; -; mRNA.
DR CCDS; CCDS23461.1; -.
DR RefSeq; NP_082995.1; NM_028719.1.
DR AlphaFoldDB; Q8BLR2; -.
DR SMR; Q8BLR2; -.
DR BioGRID; 216429; 7.
DR IntAct; Q8BLR2; 1.
DR STRING; 10090.ENSMUSP00000049663; -.
DR iPTMnet; Q8BLR2; -.
DR PhosphoSitePlus; Q8BLR2; -.
DR jPOST; Q8BLR2; -.
DR MaxQB; Q8BLR2; -.
DR PaxDb; Q8BLR2; -.
DR PeptideAtlas; Q8BLR2; -.
DR PRIDE; Q8BLR2; -.
DR ProteomicsDB; 283937; -.
DR Antibodypedia; 33351; 88 antibodies from 19 providers.
DR DNASU; 74020; -.
DR Ensembl; ENSMUST00000057742; ENSMUSP00000049663; ENSMUSG00000032564.
DR GeneID; 74020; -.
DR KEGG; mmu:74020; -.
DR UCSC; uc009rhr.1; mouse.
DR CTD; 131034; -.
DR MGI; MGI:1921270; Cpne4.
DR VEuPathDB; HostDB:ENSMUSG00000032564; -.
DR eggNOG; KOG1327; Eukaryota.
DR GeneTree; ENSGT00940000155519; -.
DR HOGENOM; CLU_020452_4_0_1; -.
DR InParanoid; Q8BLR2; -.
DR OMA; XVIAEEL; -.
DR OrthoDB; 1067545at2759; -.
DR PhylomeDB; Q8BLR2; -.
DR TreeFam; TF316419; -.
DR BioGRID-ORCS; 74020; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Cpne4; mouse.
DR PRO; PR:Q8BLR2; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q8BLR2; protein.
DR Bgee; ENSMUSG00000032564; Expressed in dentate gyrus of hippocampal formation granule cell and 89 other tissues.
DR ExpressionAtlas; Q8BLR2; baseline and differential.
DR Genevisible; Q8BLR2; MM.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005544; F:calcium-dependent phospholipid binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0071277; P:cellular response to calcium ion; IBA:GO_Central.
DR CDD; cd04047; C2B_Copine; 1.
DR CDD; cd01459; vWA_copine_like; 1.
DR Gene3D; 2.60.40.150; -; 2.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR037768; C2B_Copine.
DR InterPro; IPR045052; Copine.
DR InterPro; IPR010734; Copine_C.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR10857; PTHR10857; 1.
DR Pfam; PF00168; C2; 2.
DR Pfam; PF07002; Copine; 1.
DR SMART; SM00239; C2; 2.
DR SMART; SM00327; VWA; 1.
DR SUPFAM; SSF49562; SSF49562; 2.
DR SUPFAM; SSF53300; SSF53300; 1.
DR PROSITE; PS50004; C2; 2.
PE 1: Evidence at protein level;
KW Calcium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..557
FT /note="Copine-4"
FT /id="PRO_0000144842"
FT DOMAIN 3..131
FT /note="C2 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 137..264
FT /note="C2 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 305..507
FT /note="VWFA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT BINDING 170
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 170
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 176
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 232
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 232
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 234
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 234
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 240
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
SQ SEQUENCE 557 AA; 62408 MW; B9AAD11F3777F14F CRC64;
MKKMSNIYES AANTLGIFNS PCLTKVELRV ACKGISDRDA LSKPDPCVIL KMQSHGQWFE
VDRTEVIRTC INPVYSKLFT VDFYFEEVQR LRFEVHDISS NHNGLKEADF LGGMECTLGQ
IVSQRKLSKS LLKHGNTAGK SSITVIAEEL SGNDDYVELA FNARKLDDKD FFSKSDPFLE
IFRMNDDATQ QLVHRTEVVM NNLSPAWKSF KVSVNSLCSG DPDRRLKCIV WDWDSNGKHD
FIGEFTSTFK EMRGAMEGKQ VQWECINPKY KAKKKNYKNS GMVILNQCKI HKMHSFLDYI
MGGCQIQFTV AIDFTASNGD PRNSCSLHYI HPYQPNEYLK ALVAVGEICQ DYDSDKMFPA
FGFGARIPPE YTVSHDFAIN FNEDNPECAG IQGVVEAYQS CLPKLQLYGP TNIAPIIQKV
AKSASEETNT KEASQYFILL ILTDGVITDM ADTREAIVHA SHLPMSVIIV GVGNADFSDM
QMLDGDDGIL RSPKGEPVLR DIVQFVPFRN FKHASPAALA KSVLAEVPNQ VVDYYNGKGI
KPKCSSEVYE SSRTLAP
