CPNE_DICDI
ID CPNE_DICDI Reviewed; 579 AA.
AC Q54FY7;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Copine-E;
GN Name=cpnE; ORFNames=DDB_G0290529;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP DEVELOPMENTAL STAGE.
RX PubMed=17259548; DOI=10.1128/ec.00322-06;
RA Damer C.K., Bayeva M., Kim P.S., Ho L.K., Eberhardt E.S., Socec C.I.,
RA Lee J.S., Bruce E.A., Goldman-Yassen A.E., Naliboff L.C.;
RT "Copine A is required for cytokinesis, contractile vacuole function, and
RT development in Dictyostelium.";
RL Eukaryot. Cell 6:430-442(2007).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00041};
CC -!- DEVELOPMENTAL STAGE: Expressed at relatively stable levels until 12
CC hours of development and then goes up at the 14th hour and then
CC decrease again. {ECO:0000269|PubMed:17259548}.
CC -!- SIMILARITY: Belongs to the copine family. {ECO:0000305}.
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DR EMBL; AAFI02000164; EAL62125.1; -; Genomic_DNA.
DR RefSeq; XP_635630.1; XM_630538.1.
DR AlphaFoldDB; Q54FY7; -.
DR SMR; Q54FY7; -.
DR PaxDb; Q54FY7; -.
DR PRIDE; Q54FY7; -.
DR EnsemblProtists; EAL62125; EAL62125; DDB_G0290529.
DR GeneID; 8627702; -.
DR KEGG; ddi:DDB_G0290529; -.
DR dictyBase; DDB_G0290529; cpnE.
DR eggNOG; KOG1327; Eukaryota.
DR HOGENOM; CLU_020452_3_2_1; -.
DR InParanoid; Q54FY7; -.
DR OMA; LMILVYF; -.
DR PhylomeDB; Q54FY7; -.
DR Reactome; R-DDI-1483206; Glycerophospholipid biosynthesis.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR PRO; PR:Q54FY7; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:dictyBase.
DR GO; GO:0005544; F:calcium-dependent phospholipid binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; ISS:dictyBase.
DR GO; GO:0071277; P:cellular response to calcium ion; IDA:dictyBase.
DR CDD; cd04047; C2B_Copine; 1.
DR Gene3D; 2.60.40.150; -; 2.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR037768; C2B_Copine.
DR InterPro; IPR045052; Copine.
DR InterPro; IPR010734; Copine_C.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR10857; PTHR10857; 1.
DR Pfam; PF00168; C2; 2.
DR Pfam; PF07002; Copine; 1.
DR SMART; SM00239; C2; 2.
DR SMART; SM00327; VWA; 1.
DR SUPFAM; SSF49562; SSF49562; 2.
DR SUPFAM; SSF53300; SSF53300; 1.
DR PROSITE; PS50004; C2; 2.
DR PROSITE; PS50234; VWFA; 1.
PE 2: Evidence at transcript level;
KW Calcium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..579
FT /note="Copine-E"
FT /id="PRO_0000330658"
FT DOMAIN 45..175
FT /note="C2 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 183..304
FT /note="C2 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 345..552
FT /note="VWFA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT BINDING 80
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 80
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 86
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 145
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 145
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 147
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 147
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 153
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
SQ SEQUENCE 579 AA; 64348 MW; D6DA0AC095A334D5 CRC64;
MSDSSIVNLS KEGDSNFSSA TINLSKVDDT VNVSNATINL SKVTIDPSNQ MSNPSNQGIN
LSKCTKVELR FKCMNLKDMD TFSKSDPQIF VYEKKGNNNN NNNSKPILIG STEMISNNLN
PVFKKTVTID YHFERIQNLK FEVLDIDGGG KNDTIGDFSI TLGNIISKPG KKVIGEIKCN
GKQTGTIEII AEEIQETGQN IILKLQGSKL DKKDLFSSDP FFKIFKSSAN GNLLVYQSPV
IKSNINPIYN PIIFKLEEFN GGDMFRELTF EFWDYDTIGD NDFIGSFKTT TDEILKGQVR
EFTLINPKKL SKSSYKNSGK IVFTDARLIA QPTFIDYLSG GCEINLMIAI DCTASNGMPS
DRTSLHYNTP THESEYARSI LAVGNVLAPY DSDGKIELLG FGAERFGGVT SHCFQFGPKA
EARGIEEVLS TYNKVIPTIK LSYPTNFQEI IKHAHKKSIK GVDSKNQKYT ILLILTDGEI
TDMEETIEEI VKASSKAPLS IVIVGVGIAS FELMKKLDGD ENQLSDKNGV IATRDIVQFV
PFKKYENDPE QLAAETLCEI PEQLIGYMKS QNYFPNMKN
