CPN_DROME
ID CPN_DROME Reviewed; 864 AA.
AC Q02910; B5X510; Q95U45; Q9VGC8; Q9VGC9;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Calphotin;
GN Name=Cpn; Synonyms=cap; ORFNames=CG4795;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC STRAIN=Canton-S; TISSUE=Head;
RX PubMed=8094559; DOI=10.1073/pnas.90.4.1531;
RA Martin J.H., Benzer S., Rudnicka M., Miller C.A.;
RT "Calphotin: a Drosophila photoreceptor cell calcium-binding protein.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:1531-1535(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM B), FUNCTION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=Canton-S; TISSUE=Retinal photoreceptor;
RX PubMed=8434015; DOI=10.1073/pnas.90.4.1536;
RA Ballinger D.G., Xue N., Harshman K.D.;
RT "A Drosophila photoreceptor cell-specific protein, calphotin, binds calcium
RT and contains a leucine zipper.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:1536-1540(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B), AND RNA EDITING OF
RP POSITION 402.
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP RNA EDITING OF POSITION 402.
RX PubMed=17018572; DOI=10.1261/rna.254306;
RA Stapleton M., Carlson J.W., Celniker S.E.;
RT "RNA editing in Drosophila melanogaster: new targets and functional
RT consequences.";
RL RNA 12:1922-1932(2006).
CC -!- FUNCTION: Plays important roles in both rhabdomere development and in
CC photoreceptor cell survival. Might function as a calcium-sequestering
CC 'sponge' to regulate the amount of free cytoplasmic calcium. It binds
CC 0.3 mole of Ca(2+) per mole of protein. {ECO:0000269|PubMed:8094559,
CC ECO:0000269|PubMed:8434015}.
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:8094559}.
CC Note=Hypodense compartment.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B;
CC IsoId=Q02910-1; Sequence=Displayed;
CC Name=A;
CC IsoId=Q02910-2; Sequence=VSP_013780;
CC -!- TISSUE SPECIFICITY: Soma and axons of photoreceptor cells of compound
CC eyes and ocelli. {ECO:0000269|PubMed:8094559,
CC ECO:0000269|PubMed:8434015}.
CC -!- DEVELOPMENTAL STAGE: Expressed early in photoreceptor cell development.
CC -!- RNA EDITING: Modified_positions=402 {ECO:0000269|PubMed:12537569,
CC ECO:0000269|PubMed:17018572}; Note=Partially edited. Target of Adar.;
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DR EMBL; L02111; AAA28405.1; -; mRNA.
DR EMBL; L05080; AAA28420.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF54754.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF54755.1; -; Genomic_DNA.
DR EMBL; AY058315; AAL13544.1; -; mRNA.
DR EMBL; BT046129; ACI46517.1; -; mRNA.
DR PIR; A47282; A47282.
DR PIR; A47283; A47283.
DR RefSeq; NP_731673.1; NM_169454.2. [Q02910-2]
DR RefSeq; NP_731674.1; NM_169455.2. [Q02910-1]
DR AlphaFoldDB; Q02910; -.
DR BioGRID; 66588; 26.
DR IntAct; Q02910; 3.
DR STRING; 7227.FBpp0082021; -.
DR PaxDb; Q02910; -.
DR PRIDE; Q02910; -.
DR DNASU; 41474; -.
DR EnsemblMetazoa; FBtr0082547; FBpp0082020; FBgn0261714. [Q02910-2]
DR EnsemblMetazoa; FBtr0082548; FBpp0082021; FBgn0261714. [Q02910-1]
DR GeneID; 41474; -.
DR KEGG; dme:Dmel_CG4795; -.
DR CTD; 41474; -.
DR FlyBase; FBgn0261714; Cpn.
DR VEuPathDB; VectorBase:FBgn0261714; -.
DR eggNOG; KOG1216; Eukaryota.
DR InParanoid; Q02910; -.
DR OMA; TNYEGQQ; -.
DR PhylomeDB; Q02910; -.
DR SignaLink; Q02910; -.
DR BioGRID-ORCS; 41474; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41474; -.
DR PRO; PR:Q02910; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0261714; Expressed in head capsule and 7 other tissues.
DR Genevisible; Q02910; DM.
DR GO; GO:0030424; C:axon; IDA:FlyBase.
DR GO; GO:0044297; C:cell body; IDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005509; F:calcium ion binding; IDA:FlyBase.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0042052; P:rhabdomere development; IMP:FlyBase.
DR GO; GO:0051208; P:sequestering of calcium ion; IDA:FlyBase.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calcium; Cytoplasm; Reference proteome; RNA editing;
KW Sensory transduction; Vision.
FT CHAIN 1..864
FT /note="Calphotin"
FT /id="PRO_0000079290"
FT REGION 816..858
FT /note="Leucine-zipper"
FT VAR_SEQ 644..665
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000305"
FT /id="VSP_013780"
FT VARIANT 402
FT /note="S -> G (in RNA edited version)"
FT CONFLICT 36
FT /note="A -> AVAPAVVA (in Ref. 2; AAA28420)"
FT /evidence="ECO:0000305"
FT CONFLICT 43
FT /note="I -> T (in Ref. 2; AAA28420)"
FT /evidence="ECO:0000305"
FT CONFLICT 64
FT /note="I -> V (in Ref. 2; AAA28420)"
FT /evidence="ECO:0000305"
FT CONFLICT 76
FT /note="A -> T (in Ref. 1; AAA28405)"
FT /evidence="ECO:0000305"
FT CONFLICT 101
FT /note="Missing (in Ref. 1; AAA28405)"
FT /evidence="ECO:0000305"
FT CONFLICT 127..128
FT /note="AP -> VQ (in Ref. 1; AAA28405)"
FT /evidence="ECO:0000305"
FT CONFLICT 155
FT /note="V -> I (in Ref. 1; AAA28405)"
FT /evidence="ECO:0000305"
FT CONFLICT 161
FT /note="T -> S (in Ref. 1; AAA28405)"
FT /evidence="ECO:0000305"
FT CONFLICT 535
FT /note="A -> E (in Ref. 2; AAA28420)"
FT /evidence="ECO:0000305"
FT CONFLICT 646..647
FT /note="YP -> FPEA (in Ref. 1; AAA28405 and 2; AAA28420)"
FT /evidence="ECO:0000305"
FT CONFLICT 698
FT /note="T -> I (in Ref. 1; AAA28405)"
FT /evidence="ECO:0000305"
FT CONFLICT 702
FT /note="L -> V (in Ref. 1; AAA28405)"
FT /evidence="ECO:0000305"
FT CONFLICT 720
FT /note="E -> D (in Ref. 1; AAA28405)"
FT /evidence="ECO:0000305"
FT CONFLICT 784
FT /note="A -> P (in Ref. 1; AAA28405 and 2; AAA28420)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 864 AA; 84596 MW; 5B3635A283E5C658 CRC64;
MEPGTIPSPV SAPVAAPVTP SAVAAPVQVV SPAAVAPAPA APIAVTPVAP PPTLASVQPA
TVTIPAPAPI AAASVAPVAS VAPPVVAAPT PPAASPVSTP PVAVAQIPVA VSAPVAPPVA
ATPTPVAPIP VAAPVIATPP VAASAPTPAA VTPVVSPVIA TPPVVPANTT VPVAAPVAAV
PAAVPVVAPV LAPAVAPAVA PVVAETPAPP PVAEIPVATI PECVAPLIPE VSVVATKPLA
AAEPVVVAPP ATETPVVAPA AASPHVSVAP AVETAVVAPV SASTEPPVAA ATLTTAPETP
ALAPVVAESQ VAANTVVATP PTPAPEPETI APPVVAETPE VASVAVAETT PPVVPPVAAE
SIPAPVVATT PVPATLAVTD PDVTASAVPE LPPVIAPSPV PSAVAETPVD LAPPVLPPVA
AEPVPAVVAE ETPETPAPAS APVTIAALDI PEVAPVIAAP SDAPAEAPSA AAPIVSTPPT
TASVPETTAP PAAVPTEPID VSVLSEAAIE TPVAPPVEVT TEVAVADVAP PEAAADLIIE
PVEPPAPIPD LLEQTTSVPA VEAAESTSSP IPETSLPPPN EAVASPEVAV APITAPEPIP
EPEPSLATPT EPIPVEAPVV IQEAVDAVEV PVTETSTSIP ETTVEYPVAE KVLDPAITEA
PVTTQEPDVA NINDGAPATE ITTPAVEIVT AAAEVSDTAI PLIDPPVPQE IAVAEIPETE
TKPAEVIVEQ STIPIEAPVP EVSKYAEPVI SEAPAAEVPI TAGDNPDNTS VGISEVVPTI
AEKAVEEVPT SEIPEQSSSP SDSVPVAKIT PLLRDLQTTD VSLLAIAATL DAIGEKLKDQ
KARNQQVMDR LCEIEKILGP PKSN
