CPO_DROME
ID CPO_DROME Reviewed; 606 AA.
AC Q01617; A4V319; E1JIN7; Q59DW5; Q59DW6; Q8IGV9; Q8MQI9; Q9VED9; Q9VEE1;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 06-JUL-2016, sequence version 4.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Protein couch potato;
GN Name=cpo; ORFNames=CG43738;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, INITIATION CODON CTC, AND DISRUPTION PHENOTYPE.
RX PubMed=1427076; DOI=10.1101/gad.6.11.2125;
RA Bellen H.J., Kooyer S., D'Evelyn D., Pearlman J.;
RT "The Drosophila couch potato protein is expressed in nuclei of peripheral
RT neuronal precursors and shows homology to RNA-binding proteins.";
RL Genes Dev. 6:2125-2136(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP VARIANT VAL-347.
RC STRAIN=DPF;
RX PubMed=18852464; DOI=10.1073/pnas.0805485105;
RA Schmidt P.S., Zhu C.T., Das J., Batavia M., Yang L., Eanes W.F.;
RT "An amino acid polymorphism in the couch potato gene forms the basis for
RT climatic adaptation in Drosophila melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:16207-16211(2008).
CC -!- FUNCTION: May play a role in the development or function of the
CC peripheral nervous system by regulating the processing of nervous
CC system-specific transcripts. {ECO:0000269|PubMed:1427076}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:1427076}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=N {ECO:0000312|FlyBase:FBgn0263995}, U
CC {ECO:0000312|FlyBase:FBgn0263995}, Y {ECO:0000312|FlyBase:FBgn0263995};
CC IsoId=Q01617-4; Sequence=Displayed;
CC Name=2; Synonyms=T {ECO:0000312|FlyBase:FBgn0263995}, V
CC {ECO:0000312|FlyBase:FBgn0263995};
CC IsoId=Q01617-6; Sequence=VSP_058445;
CC -!- TISSUE SPECIFICITY: Expressed in neural precursors and their daughter
CC cells in the embryonic peripheral nervous system. Less abundant in a
CC number of glial cells in the peripheral and central nervous systems and
CC also present at low levels in the developing gut.
CC {ECO:0000269|PubMed:1427076}.
CC -!- DISRUPTION PHENOTYPE: Mutations cause hypoactive behavior in adults.
CC {ECO:0000269|PubMed:1427076}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF55483.6; Type=Erroneous gene model prediction; Note=Stop codon readthrough.; Evidence={ECO:0000305};
CC Sequence=AAM76197.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};
CC Sequence=AAM76197.1; Type=Miscellaneous discrepancy; Note=Unusual initiator. The initiator methionine is coded by a non-canonical CTC leucine codon.; Evidence={ECO:0000305};
CC Sequence=AAN71324.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAN71324.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAN71324.1; Type=Miscellaneous discrepancy; Note=Unusual initiator. The initiator methionine is coded by a non-canonical CTC leucine codon.; Evidence={ECO:0000305};
CC Sequence=AAX52960.2; Type=Erroneous gene model prediction; Note=Stop codon readthrough.; Evidence={ECO:0000305};
CC Sequence=CAA78663.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAA78663.1; Type=Miscellaneous discrepancy; Note=Unusual initiator. The initiator methionine is coded by a non-canonical CTC leucine codon.; Evidence={ECO:0000305};
CC Sequence=CAA78664.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAA78664.1; Type=Miscellaneous discrepancy; Note=Unusual initiator. The initiator methionine is coded by a non-canonical CTC leucine codon.; Evidence={ECO:0000305};
CC Sequence=CAA78696.1; Type=Miscellaneous discrepancy; Note=Unusual initiator. The initiator methionine is coded by a non-canonical CTC leucine codon.; Evidence={ECO:0000305};
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DR EMBL; Z14311; CAA78663.1; ALT_SEQ; Genomic_DNA.
DR EMBL; Z14312; CAA78664.1; ALT_SEQ; Genomic_DNA.
DR EMBL; Z14974; CAA78696.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AE014297; AAF55483.6; ALT_SEQ; Genomic_DNA.
DR EMBL; AE014297; AAF55484.6; -; Genomic_DNA.
DR EMBL; AE014297; AAF55485.6; -; Genomic_DNA.
DR EMBL; AE014297; AAN13754.5; -; Genomic_DNA.
DR EMBL; AE014297; AAX52960.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AE014297; AAX52961.2; -; Genomic_DNA.
DR EMBL; AE014297; ACZ94936.2; -; Genomic_DNA.
DR EMBL; AY129455; AAM76197.1; ALT_SEQ; mRNA.
DR EMBL; BT001569; AAN71324.1; ALT_FRAME; mRNA.
DR PIR; A46230; A46230.
DR PIR; S24761; S24761.
DR RefSeq; NP_001014631.3; NM_001014631.3. [Q01617-6]
DR RefSeq; NP_001014632.3; NM_001014632.4.
DR RefSeq; NP_001163640.2; NM_001170169.3. [Q01617-6]
DR RefSeq; NP_001262691.1; NM_001275762.1. [Q01617-4]
DR RefSeq; NP_001262692.1; NM_001275763.1. [Q01617-4]
DR RefSeq; NP_524844.6; NM_080105.4. [Q01617-4]
DR RefSeq; NP_732282.6; NM_169781.4.
DR RefSeq; NP_732283.5; NM_169782.3. [Q01617-4]
DR RefSeq; NP_732284.5; NM_169783.4. [Q01617-4]
DR AlphaFoldDB; Q01617; -.
DR SMR; Q01617; -.
DR BioGRID; 69919; 8.
DR IntAct; Q01617; 1.
DR STRING; 7227.FBpp0303307; -.
DR PaxDb; Q01617; -.
DR PRIDE; Q01617; -.
DR EnsemblMetazoa; FBtr0330269; FBpp0303301; FBgn0263995. [Q01617-4]
DR EnsemblMetazoa; FBtr0330275; FBpp0303307; FBgn0263995. [Q01617-6]
DR EnsemblMetazoa; FBtr0330276; FBpp0303308; FBgn0263995. [Q01617-4]
DR EnsemblMetazoa; FBtr0330277; FBpp0303309; FBgn0263995. [Q01617-6]
DR EnsemblMetazoa; FBtr0330278; FBpp0303310; FBgn0263995. [Q01617-4]
DR EnsemblMetazoa; FBtr0330279; FBpp0303311; FBgn0263995. [Q01617-4]
DR EnsemblMetazoa; FBtr0330280; FBpp0303312; FBgn0263995. [Q01617-4]
DR GeneID; 45840; -.
DR KEGG; dme:Dmel_CG43738; -.
DR UCSC; CG31243-RB; d. melanogaster.
DR UCSC; CG31243-RG; d. melanogaster.
DR UCSC; CG31243-RH; d. melanogaster.
DR CTD; 130749; -.
DR FlyBase; FBgn0263995; cpo.
DR VEuPathDB; VectorBase:FBgn0263995; -.
DR eggNOG; KOG1457; Eukaryota.
DR InParanoid; Q01617; -.
DR BioGRID-ORCS; 45840; 0 hits in 3 CRISPR screens.
DR ChiTaRS; cpo; fly.
DR GenomeRNAi; 45840; -.
DR PRO; PR:Q01617; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0263995; Expressed in antenna and 37 other tissues.
DR ExpressionAtlas; Q01617; baseline and differential.
DR Genevisible; Q01617; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0003729; F:mRNA binding; ISS:FlyBase.
DR GO; GO:0007268; P:chemical synaptic transmission; IMP:FlyBase.
DR GO; GO:0022611; P:dormancy process; IMP:FlyBase.
DR GO; GO:0035206; P:regulation of hemocyte proliferation; IMP:FlyBase.
DR CDD; cd12684; RRM_cpo; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR034788; Cpo_RRM.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; Nucleus; Reference proteome;
KW Repeat; RNA-binding.
FT CHAIN 1..606
FT /note="Protein couch potato"
FT /id="PRO_0000081520"
FT REPEAT 91..95
FT /note="1"
FT REPEAT 109..113
FT /note="2"
FT REPEAT 114..118
FT /note="3"
FT REPEAT 122..126
FT /note="4"
FT REPEAT 128..132
FT /note="5"
FT REPEAT 134..138
FT /note="6"
FT REPEAT 159..163
FT /note="7"
FT DOMAIN 442..524
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 91..164
FT /note="7 X 5 AA approximate repeats of P-V-S-V-P"
FT REGION 147..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 282..311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 324..365
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 388..410
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 81..105
FT /note="Nuclear localization signal"
FT COMPBIAS 341..365
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 505
FT /note="Q -> QQ (in isoform 2)"
FT /id="VSP_058445"
FT VARIANT 347
FT /note="A -> V (in strain: DPF; induces a high-diapause
FT phenotype)"
FT CONFLICT 14
FT /note="A -> D (in Ref. 1; CAA78663/CAA78664/CAA78696)"
FT /evidence="ECO:0000305"
FT CONFLICT 169
FT /note="S -> T (in Ref. 1; CAA78663/CAA78664/CAA78696)"
FT /evidence="ECO:0000305"
FT CONFLICT 302
FT /note="N -> T (in Ref. 1; CAA78663/CAA78664/CAA78696)"
FT /evidence="ECO:0000305"
FT CONFLICT 310
FT /note="A -> G (in Ref. 1; CAA78663/CAA78664/CAA78696)"
FT /evidence="ECO:0000305"
FT CONFLICT 587..588
FT /note="QH -> HS (in Ref. 1; CAA78696)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 606 AA; 63296 MW; B27A0F7F0E774FCE CRC64;
MLGSHHQLLI AATAAAAAAA AAEPQLQLQH LLPAAPTTPA VISNPINSIG PINQISSSSH
PSNNNQQAVF EKAITISSIA IKRRPTLPQT PASAPQVLSP SPKRQCAAAV SVLPVTVPVP
VPVSVPLPVS VPVPVSVKGH PISHTHQIAH THQISHSHPI SHPHHHQLSF AHPTQFAAAV
AAHHQQQQQQ QAQQQQQAVQ QQQQQAVQQQ QVAYAVAASP QLQQQQQQQQ HRLAQFNQAA
AAALLNQHLQ QQHQAQQQQH QAQQQSLAHY GGYQLHRYAP QQQQQHILLS SGSSSSKHNS
NNNSNTSAGA ASAAVPIATS VAAVPTTGGS LPDSPAHESH SHESNSATAS APTTPSPAGS
VTSAAPTATA TAAAAGSAAA TAAATGTPAT SAVSDSNNNL NSSSSSNSNS NAIMENQMAL
APLGLSQSMD SVNTASNEEE VRTLFVSGLP MDAKPRELYL LFRAYEGYEG SLLKVTSKNG
KTASPVGFVT FHTRAGAEAA KQDLQGVRFD PDMPQTIRLE FAKSNTKVSK PKPQPNTATT
ASHPALMHPL TGHLGGPFFP GGPELWHHPL AYSAAAAAEL PGAAALQHAT LVHPALHPQV
PVRSYL
