CPPED_MOUSE
ID CPPED_MOUSE Reviewed; 312 AA.
AC Q8BFS6; Q3UYF7; Q8BJQ3; Q8BYB7; Q8K236; Q8R3G2;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Serine/threonine-protein phosphatase CPPED1;
DE EC=3.1.3.16;
DE AltName: Full=Calcineurin-like phosphoesterase domain-containing protein 1;
GN Name=Cpped1; Synonyms=Cstp1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
RC STRAIN=C57BL/6J;
RC TISSUE=Adipose tissue, Medulla oblongata, Olfactory bulb, Spinal cord, and
RC Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 2-312 (ISOFORM 1).
RC STRAIN=FVB/N, and FVB/N-3; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Protein phosphatase that dephosphorylates AKT family kinase
CC specifically at 'Ser-473', blocking cell cycle progression and
CC promoting cell apoptosis. May play an inhibitory role in glucose uptake
CC by adipocytes (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:83421; EC=3.1.3.16;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:61977; EC=3.1.3.16;
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000250};
CC Note=Binds 2 divalent metal cations. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=Q8BFS6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BFS6-2; Sequence=VSP_031660;
CC Name=3;
CC IsoId=Q8BFS6-3; Sequence=VSP_031661, VSP_031663;
CC Name=4;
CC IsoId=Q8BFS6-4; Sequence=VSP_031664, VSP_031665;
CC Name=5;
CC IsoId=Q8BFS6-5; Sequence=VSP_031659, VSP_031662;
CC -!- SIMILARITY: Belongs to the metallophosphoesterase superfamily. CPPED1
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH25476.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK041329; BAC30908.1; -; mRNA.
DR EMBL; AK032288; BAC27795.1; -; mRNA.
DR EMBL; AK049721; BAC33892.1; -; mRNA.
DR EMBL; AK080876; BAC38056.1; -; mRNA.
DR EMBL; AK134718; BAE22255.1; -; mRNA.
DR EMBL; BC034347; AAH34347.1; -; mRNA.
DR EMBL; BC025476; AAH25476.1; ALT_INIT; mRNA.
DR CCDS; CCDS27964.1; -. [Q8BFS6-1]
DR RefSeq; NP_666179.2; NM_146067.3. [Q8BFS6-1]
DR AlphaFoldDB; Q8BFS6; -.
DR SMR; Q8BFS6; -.
DR STRING; 10090.ENSMUSP00000093992; -.
DR iPTMnet; Q8BFS6; -.
DR PhosphoSitePlus; Q8BFS6; -.
DR SwissPalm; Q8BFS6; -.
DR EPD; Q8BFS6; -.
DR jPOST; Q8BFS6; -.
DR MaxQB; Q8BFS6; -.
DR PaxDb; Q8BFS6; -.
DR PeptideAtlas; Q8BFS6; -.
DR PRIDE; Q8BFS6; -.
DR ProteomicsDB; 278022; -. [Q8BFS6-1]
DR ProteomicsDB; 278023; -. [Q8BFS6-2]
DR ProteomicsDB; 278024; -. [Q8BFS6-3]
DR ProteomicsDB; 278025; -. [Q8BFS6-4]
DR ProteomicsDB; 278026; -. [Q8BFS6-5]
DR Antibodypedia; 24789; 144 antibodies from 23 providers.
DR DNASU; 223978; -.
DR Ensembl; ENSMUST00000073371; ENSMUSP00000089104; ENSMUSG00000065979. [Q8BFS6-3]
DR Ensembl; ENSMUST00000096272; ENSMUSP00000093992; ENSMUSG00000065979. [Q8BFS6-1]
DR Ensembl; ENSMUST00000121750; ENSMUSP00000112587; ENSMUSG00000065979. [Q8BFS6-2]
DR GeneID; 223978; -.
DR KEGG; mmu:223978; -.
DR UCSC; uc007yfm.1; mouse. [Q8BFS6-1]
DR UCSC; uc007yfn.1; mouse. [Q8BFS6-2]
DR UCSC; uc007yfo.1; mouse. [Q8BFS6-4]
DR UCSC; uc007yfp.1; mouse. [Q8BFS6-3]
DR CTD; 55313; -.
DR MGI; MGI:2443300; Cpped1.
DR VEuPathDB; HostDB:ENSMUSG00000065979; -.
DR eggNOG; KOG1378; Eukaryota.
DR GeneTree; ENSGT00390000008676; -.
DR HOGENOM; CLU_1712684_0_0_1; -.
DR InParanoid; Q8BFS6; -.
DR OMA; YENPSKC; -.
DR OrthoDB; 908967at2759; -.
DR PhylomeDB; Q8BFS6; -.
DR TreeFam; TF329406; -.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 223978; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Cpped1; mouse.
DR PRO; PR:Q8BFS6; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q8BFS6; protein.
DR Bgee; ENSMUSG00000065979; Expressed in interventricular septum and 249 other tissues.
DR ExpressionAtlas; Q8BFS6; baseline and differential.
DR Genevisible; Q8BFS6; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR CDD; cd07395; MPP_CSTP1; 1.
DR Gene3D; 3.60.21.10; -; 1.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR InterPro; IPR041867; MPP_CSTP1.
DR Pfam; PF00149; Metallophos; 1.
DR SUPFAM; SSF56300; SSF56300; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Hydrolase; Metal-binding; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..312
FT /note="Serine/threonine-protein phosphatase CPPED1"
FT /id="PRO_0000320557"
FT REGION 47..250
FT /note="Catalytic"
FT /evidence="ECO:0000250"
FT BINDING 90
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 90
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 127
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 246
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT MOD_RES 2
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BRF8"
FT MOD_RES 293
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BRF8"
FT VAR_SEQ 1..138
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_031659"
FT VAR_SEQ 24..37
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_031660"
FT VAR_SEQ 98..153
FT /note="TPWRQEQTRDLQRVLKAVDQDIPLVMVSGNHDLGNAPTAETVEEFCQTWGDD
FT YFSF -> GKGKAGGKRKGASEEEPEEEVLGYISSRCFWNSLPSPRPSLSTVLSQAGPQ
FT PRDQV (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_031661"
FT VAR_SEQ 139..160
FT /note="VEEFCQTWGDDYFSFWVGGVLF -> MERSILLRPRRRHSVWTDEG (in
FT isoform 5)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_031662"
FT VAR_SEQ 154..312
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_031663"
FT VAR_SEQ 239..266
FT /note="IRAVFSGHYHRNAGGTYQNLDMVVSSAI -> NAWGMCMCQPPSATWRSEEG
FT TRLGWDRF (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_031664"
FT VAR_SEQ 267..312
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_031665"
SQ SEQUENCE 312 AA; 35248 MW; F3D4CB7AF800265B CRC64;
MSAMEAADVF HRARGRTLDA FSSEKEREWK GPFYFVQGAD TQFGLMKAWS TGNCDAGGDE
WGQEIRLTEQ AVEAINKLNP KPKFFVLCGD LVHAMPGTPW RQEQTRDLQR VLKAVDQDIP
LVMVSGNHDL GNAPTAETVE EFCQTWGDDY FSFWVGGVLF LVLNSQFLYD ASRCPALKQA
QDHWLDQQLN IAEQKQCQHA IVFQHIPLFL QSIDEDDDYF NLTKTVRKEL AEKLTRAGIR
AVFSGHYHRN AGGTYQNLDM VVSSAIGCQL GKDTHGLRVV AITAEKIVHR YYSLDELSQG
GVEEDLKELL KE
